Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge
Southern Ocean primary productivity plays a key role in global ocean biogeochemistry and climate. At the Southern Ocean sea ice edge in coastal McMurdo Sound, we observed simultaneous cobalamin and iron limitation of surface water phytoplankton communities in late Austral summer. Cobalamin is produc...
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ftwilliammarycol:oai:scholarworks.wm.edu:vimsarticles-1833 2023-06-11T04:04:57+02:00 Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge Bertrand, EM McCrow, JP Moustafa, A Zheng, H McQuaid, JB Post, AF Sipler, RE Spackeen, JL Xu, K Bronk, DA Hutchins, DA Allen, AE 2015-01-01T08:00:00Z application/pdf https://scholarworks.wm.edu/vimsarticles/833 doi: 10.1073/pnas.1501615112 https://scholarworks.wm.edu/context/vimsarticles/article/1833/viewcontent/9938.full.pdf unknown W&M ScholarWorks https://scholarworks.wm.edu/vimsarticles/833 doi: 10.1073/pnas.1501615112 https://scholarworks.wm.edu/context/vimsarticles/article/1833/viewcontent/9938.full.pdf VIMS Articles Ross Sea Summer Bacterioplankton Community Structure Iron Limitation Surface Waters Vitamin-B-12 Identification Dimethylsulfide Metabolism Flavodoxin Physical Sciences Peer-Reviewed Articles Aquaculture and Fisheries text 2015 ftwilliammarycol https://doi.org/10.1073/pnas.1501615112 2023-05-04T17:49:10Z Southern Ocean primary productivity plays a key role in global ocean biogeochemistry and climate. At the Southern Ocean sea ice edge in coastal McMurdo Sound, we observed simultaneous cobalamin and iron limitation of surface water phytoplankton communities in late Austral summer. Cobalamin is produced only by bacteria and archaea, suggesting phytoplankton-bacterial interactions must play a role in this limitation. To characterize these interactions and investigate the molecular basis of multiple nutrient limitation, we examined transitions in global gene expression over short time scales, induced by shifts in micronutrient availability. Diatoms, the dominant primary producers, exhibited transcriptional patterns indicative of co-occurring iron and cobalamin deprivation. The major contributor to cobalamin biosynthesis gene expression was a gammaproteobacterial population, Oceanospirillaceae ASP10-02a. This group also contributed significantly to metagenomic cobalamin biosynthesis gene abundance throughout Southern Ocean surface waters. Oceanospirillaceae ASP10-02a displayed elevated expression of organic matter acquisition and cell surface attachment-related genes, consistent with a mutualistic relationship in which they are dependent on phytoplankton growth to fuel cobalamin production. Separate bacterial groups, including Methylophaga, appeared to rely on phytoplankton for carbon and energy sources, but displayed gene expression patterns consistent with iron and cobalamin deprivation. This suggests they also compete with phytoplankton and are important cobalamin consumers. Expression patterns of siderophore-related genes offer evidence for bacterial influences on iron availability as well. The nature and degree of this episodic colimitation appear to be mediated by a series of phytoplankton-bacterial interactions in both positive and negative feedback loops. Text Antarc* Antarctic McMurdo Sound Ross Sea Sea ice Southern Ocean W&M ScholarWorks Antarctic Southern Ocean Austral Ross Sea McMurdo Sound Proceedings of the National Academy of Sciences 112 32 9938 9943 |
institution |
Open Polar |
collection |
W&M ScholarWorks |
op_collection_id |
ftwilliammarycol |
language |
unknown |
topic |
Ross Sea Summer Bacterioplankton Community Structure Iron Limitation Surface Waters Vitamin-B-12 Identification Dimethylsulfide Metabolism Flavodoxin Physical Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
spellingShingle |
Ross Sea Summer Bacterioplankton Community Structure Iron Limitation Surface Waters Vitamin-B-12 Identification Dimethylsulfide Metabolism Flavodoxin Physical Sciences Peer-Reviewed Articles Aquaculture and Fisheries Bertrand, EM McCrow, JP Moustafa, A Zheng, H McQuaid, JB Post, AF Sipler, RE Spackeen, JL Xu, K Bronk, DA Hutchins, DA Allen, AE Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge |
topic_facet |
Ross Sea Summer Bacterioplankton Community Structure Iron Limitation Surface Waters Vitamin-B-12 Identification Dimethylsulfide Metabolism Flavodoxin Physical Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
description |
Southern Ocean primary productivity plays a key role in global ocean biogeochemistry and climate. At the Southern Ocean sea ice edge in coastal McMurdo Sound, we observed simultaneous cobalamin and iron limitation of surface water phytoplankton communities in late Austral summer. Cobalamin is produced only by bacteria and archaea, suggesting phytoplankton-bacterial interactions must play a role in this limitation. To characterize these interactions and investigate the molecular basis of multiple nutrient limitation, we examined transitions in global gene expression over short time scales, induced by shifts in micronutrient availability. Diatoms, the dominant primary producers, exhibited transcriptional patterns indicative of co-occurring iron and cobalamin deprivation. The major contributor to cobalamin biosynthesis gene expression was a gammaproteobacterial population, Oceanospirillaceae ASP10-02a. This group also contributed significantly to metagenomic cobalamin biosynthesis gene abundance throughout Southern Ocean surface waters. Oceanospirillaceae ASP10-02a displayed elevated expression of organic matter acquisition and cell surface attachment-related genes, consistent with a mutualistic relationship in which they are dependent on phytoplankton growth to fuel cobalamin production. Separate bacterial groups, including Methylophaga, appeared to rely on phytoplankton for carbon and energy sources, but displayed gene expression patterns consistent with iron and cobalamin deprivation. This suggests they also compete with phytoplankton and are important cobalamin consumers. Expression patterns of siderophore-related genes offer evidence for bacterial influences on iron availability as well. The nature and degree of this episodic colimitation appear to be mediated by a series of phytoplankton-bacterial interactions in both positive and negative feedback loops. |
format |
Text |
author |
Bertrand, EM McCrow, JP Moustafa, A Zheng, H McQuaid, JB Post, AF Sipler, RE Spackeen, JL Xu, K Bronk, DA Hutchins, DA Allen, AE |
author_facet |
Bertrand, EM McCrow, JP Moustafa, A Zheng, H McQuaid, JB Post, AF Sipler, RE Spackeen, JL Xu, K Bronk, DA Hutchins, DA Allen, AE |
author_sort |
Bertrand, EM |
title |
Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge |
title_short |
Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge |
title_full |
Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge |
title_fullStr |
Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge |
title_full_unstemmed |
Phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge |
title_sort |
phytoplankton-bacterial interactions mediate micronutrient colimitation at the coastal antarctic sea ice edge |
publisher |
W&M ScholarWorks |
publishDate |
2015 |
url |
https://scholarworks.wm.edu/vimsarticles/833 https://scholarworks.wm.edu/context/vimsarticles/article/1833/viewcontent/9938.full.pdf |
geographic |
Antarctic Southern Ocean Austral Ross Sea McMurdo Sound |
geographic_facet |
Antarctic Southern Ocean Austral Ross Sea McMurdo Sound |
genre |
Antarc* Antarctic McMurdo Sound Ross Sea Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic McMurdo Sound Ross Sea Sea ice Southern Ocean |
op_source |
VIMS Articles |
op_relation |
https://scholarworks.wm.edu/vimsarticles/833 doi: 10.1073/pnas.1501615112 https://scholarworks.wm.edu/context/vimsarticles/article/1833/viewcontent/9938.full.pdf |
op_doi |
https://doi.org/10.1073/pnas.1501615112 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
112 |
container_issue |
32 |
container_start_page |
9938 |
op_container_end_page |
9943 |
_version_ |
1768392154338557952 |