Wikibooks: Metabolomics/Analytical Methods/NMR/P NMR

• Format: Book
• Language: English
• Subjects: atlantic cod; Gadus morhua
• Online Access: https://en.wikibooks.org/wiki/Metabolomics/Analytical_Methods/NMR/P_NMR

Back to Previous Chapter [[ Hormones]] Next chapter [[ Computational Modeling of Metabolic Control ]] Next Category [[ Mass Spectrometry]] Go to [[ Other nuclei]] Go back to [[ C NMR]] Please add content and articles Examining the Metabolic Processes Underpinning Critical Swimming in Atlantic cod (Gadus morhua L.) using in vivo 31 P NMR Spectroscopy In a recent study in vivo 31P NMR spectroscopy was used in the examination of the metabolic processes underpinning critical swimming in Atlantic cod Critical swimming speed (Ucrit) is identified as the speed at which a fish can no longer propel itself forward and becomes exhausted. 31P NMR spectroscopy was used in combination with a Brett type swim tunnel in order to perform a detailed analysis of the processes occurring during a Ucrit swim test. The Ucrit swim test was originally intended for analyzing the dependence of salmon fitness on temperature. Brett defined this critical speed as the swimming velocity of a fish that could no longer propel itself forward as a result of exhaustion. Previous analyses of different swimming modes and metabolic processes that fuel muscle contractions in fish have established that red muscle is oxidative and produces slow contractions during subcarangiform swimming while white muscle is glycolytic and responsible for fast twitch contractions which produce tail kicks during burst swimming. Increases in inorganic phosphate and acidification of the intracellular milieu were observed during the muscular fatigue that occurs during the critical swimming speed but the exact processes involved have not been established. However the products of anaerobic kick and glide – fast twitch – bursts leave products to indicate the metabolic state of the fish. These products have allowed researches to examine the relationship between swimming gait and metabolic processes. Although the Ucrit swim test provides information about the swimming performance of Atlantic cod and metabolic products from Atlantic cod muscles during the Ucrit they do not ...