Multiple steroid and thyroid hormones detected in baleen from eight whale species
© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Conservation Physiology 5 (2017): cox061, doi:10.1093/conphys/cox061. Recent studies have demonstrated that some hormones are present in baleen powd...
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/9436 2023-05-15T15:36:01+02:00 Multiple steroid and thyroid hormones detected in baleen from eight whale species Hunt, Kathleen E. Lysiak, Nadine S. J. Robbins, Jooke Moore, Michael J. Seton, Rosemary E. Torres, Leigh Buck, C. Loren 2017-11-09 https://hdl.handle.net/1912/9436 en_US eng Oxford University Press https://doi.org/10.1093/conphys/cox061 Conservation Physiology 5 (2017): cox061 https://hdl.handle.net/1912/9436 doi:10.1093/conphys/cox061 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY Conservation Physiology 5 (2017): cox061 doi:10.1093/conphys/cox061 Baleen Cetaceans Hormones Marine mammals Reproduction Stress Article 2017 ftwhoas https://doi.org/10.1093/conphys/cox061 2022-05-28T23:00:04Z © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Conservation Physiology 5 (2017): cox061, doi:10.1093/conphys/cox061. Recent studies have demonstrated that some hormones are present in baleen powder from bowhead (Balaena mysticetus) and North Atlantic right (Eubalaena glacialis) whales. To test the potential generalizability of this technique for studies of stress and reproduction in large whales, we sought to determine whether all major classes of steroid and thyroid hormones are detectable in baleen, and whether these hormones are detectable in other mysticetes. Powdered baleen samples were recovered from single specimens of North Atlantic right, bowhead, blue (Balaenoptera [B.]musculus), sei (B. borealis), minke (B. acutorostrata), fin (B. physalus), humpback (Megaptera novaeangliae) and gray (Eschrichtius robustus) whales. Hormones were extracted with a methanol vortex method, after which we tested all species with commercial enzyme immunoassays (EIAs, Arbor Assays) for progesterone, testosterone, 17β-estradiol, cortisol, corticosterone, aldosterone, thyroxine and tri-iodothyronine, representing a wide array of steroid and thyroid hormones of interest for whale physiology research. In total, 64 parallelism tests (8 species × 8 hormones) were evaluated to verify good binding affinity of the assay antibodies to hormones in baleen. We also tested assay accuracy, although available sample volume limited this test to progesterone, testosterone and cortisol. All tested hormones were detectable in baleen powder of all species, and all assays passed parallelism and accuracy tests. Although only single individuals were tested, the consistent detectability of all hormones in all species indicates that baleen hormone analysis is likely applicable to a broad range of mysticetes, and that the EIA kits tested here perform well with baleen extract. Quantification of hormones in baleen may be a suitable technique with ... Article in Journal/Newspaper Balaena mysticetus Eubalaena glacialis Megaptera novaeangliae North Atlantic Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Eia ENVELOPE(7.755,7.755,63.024,63.024) Conservation Physiology 5 1 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
English |
topic |
Baleen Cetaceans Hormones Marine mammals Reproduction Stress |
spellingShingle |
Baleen Cetaceans Hormones Marine mammals Reproduction Stress Hunt, Kathleen E. Lysiak, Nadine S. J. Robbins, Jooke Moore, Michael J. Seton, Rosemary E. Torres, Leigh Buck, C. Loren Multiple steroid and thyroid hormones detected in baleen from eight whale species |
topic_facet |
Baleen Cetaceans Hormones Marine mammals Reproduction Stress |
description |
© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Conservation Physiology 5 (2017): cox061, doi:10.1093/conphys/cox061. Recent studies have demonstrated that some hormones are present in baleen powder from bowhead (Balaena mysticetus) and North Atlantic right (Eubalaena glacialis) whales. To test the potential generalizability of this technique for studies of stress and reproduction in large whales, we sought to determine whether all major classes of steroid and thyroid hormones are detectable in baleen, and whether these hormones are detectable in other mysticetes. Powdered baleen samples were recovered from single specimens of North Atlantic right, bowhead, blue (Balaenoptera [B.]musculus), sei (B. borealis), minke (B. acutorostrata), fin (B. physalus), humpback (Megaptera novaeangliae) and gray (Eschrichtius robustus) whales. Hormones were extracted with a methanol vortex method, after which we tested all species with commercial enzyme immunoassays (EIAs, Arbor Assays) for progesterone, testosterone, 17β-estradiol, cortisol, corticosterone, aldosterone, thyroxine and tri-iodothyronine, representing a wide array of steroid and thyroid hormones of interest for whale physiology research. In total, 64 parallelism tests (8 species × 8 hormones) were evaluated to verify good binding affinity of the assay antibodies to hormones in baleen. We also tested assay accuracy, although available sample volume limited this test to progesterone, testosterone and cortisol. All tested hormones were detectable in baleen powder of all species, and all assays passed parallelism and accuracy tests. Although only single individuals were tested, the consistent detectability of all hormones in all species indicates that baleen hormone analysis is likely applicable to a broad range of mysticetes, and that the EIA kits tested here perform well with baleen extract. Quantification of hormones in baleen may be a suitable technique with ... |
format |
Article in Journal/Newspaper |
author |
Hunt, Kathleen E. Lysiak, Nadine S. J. Robbins, Jooke Moore, Michael J. Seton, Rosemary E. Torres, Leigh Buck, C. Loren |
author_facet |
Hunt, Kathleen E. Lysiak, Nadine S. J. Robbins, Jooke Moore, Michael J. Seton, Rosemary E. Torres, Leigh Buck, C. Loren |
author_sort |
Hunt, Kathleen E. |
title |
Multiple steroid and thyroid hormones detected in baleen from eight whale species |
title_short |
Multiple steroid and thyroid hormones detected in baleen from eight whale species |
title_full |
Multiple steroid and thyroid hormones detected in baleen from eight whale species |
title_fullStr |
Multiple steroid and thyroid hormones detected in baleen from eight whale species |
title_full_unstemmed |
Multiple steroid and thyroid hormones detected in baleen from eight whale species |
title_sort |
multiple steroid and thyroid hormones detected in baleen from eight whale species |
publisher |
Oxford University Press |
publishDate |
2017 |
url |
https://hdl.handle.net/1912/9436 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Balaena mysticetus Eubalaena glacialis Megaptera novaeangliae North Atlantic |
genre_facet |
Balaena mysticetus Eubalaena glacialis Megaptera novaeangliae North Atlantic |
op_source |
Conservation Physiology 5 (2017): cox061 doi:10.1093/conphys/cox061 |
op_relation |
https://doi.org/10.1093/conphys/cox061 Conservation Physiology 5 (2017): cox061 https://hdl.handle.net/1912/9436 doi:10.1093/conphys/cox061 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1093/conphys/cox061 |
container_title |
Conservation Physiology |
container_volume |
5 |
container_issue |
1 |
_version_ |
1766366353147559936 |