Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure
© The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Marine Genomics 34 (2017): 39-...
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/9253 2023-05-15T15:55:41+02:00 Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure Thabet, Ali A. Maas, Amy E. Saber, Samy A. Tarrant, Ann M. 2017-03 https://hdl.handle.net/1912/9253 en_US eng https://doi.org/10.1016/j.margen.2017.03.003 https://hdl.handle.net/1912/9253 Ocean acidification Zooplankton Gene expression Next generation sequencing Mollusc Invertebrate Preprint 2017 ftwhoas https://doi.org/10.1016/j.margen.2017.03.003 2022-05-28T22:59:59Z © The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Marine Genomics 34 (2017): 39-45, doi:10.1016/j.margen.2017.03.003. The gymnosome (unshelled) pteropod Clione limacina is a pelagic predatory mollusc found in polar and sub-polar regions. It has been studied for its distinctive swimming behavior and as an obligate predator on the closely related thecosome (shelled) pteropods. As concern about ocean acidification increases, it becomes useful to compare the physiological responses of closely-related calcifying and non-calcifying species to acidification. The goals of this study were thus to generate a reference transcriptome for Clione limacina, to expose individuals to CO2 for a period of 3 days, and to explore differential patterns of gene expression. Our Trinity assembly contained 300,994 transcripts of which ~26% could be annotated. In total, only 41 transcripts were differentially expressed following the CO2 treatment, consistent with a limited physiological response of this species to short-term CO2 exposure. The differentially expressed genes identified in our study were largely distinct from those identified in previous studies of thecosome pteropods, although some similar transcripts were identified, suggesting that comparison of these transcriptomes and responses may provide insight into differences in OA responses among phylogenetically and functionally distinct molluscan lineages. A. Thabet is grateful for a fellowship from the Egyptian Culture and Education Bureau and for mentoring from Drs. M.M. Sarhan and M.M. Fouda. Funding for this research was provided by a National Science Foundation grant to Lawson, Maas, and Tarrant (OCE-1316040). Report Clione limacina Ocean acidification Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Marine Genomics 34 39 45 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
English |
topic |
Ocean acidification Zooplankton Gene expression Next generation sequencing Mollusc Invertebrate |
spellingShingle |
Ocean acidification Zooplankton Gene expression Next generation sequencing Mollusc Invertebrate Thabet, Ali A. Maas, Amy E. Saber, Samy A. Tarrant, Ann M. Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure |
topic_facet |
Ocean acidification Zooplankton Gene expression Next generation sequencing Mollusc Invertebrate |
description |
© The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Marine Genomics 34 (2017): 39-45, doi:10.1016/j.margen.2017.03.003. The gymnosome (unshelled) pteropod Clione limacina is a pelagic predatory mollusc found in polar and sub-polar regions. It has been studied for its distinctive swimming behavior and as an obligate predator on the closely related thecosome (shelled) pteropods. As concern about ocean acidification increases, it becomes useful to compare the physiological responses of closely-related calcifying and non-calcifying species to acidification. The goals of this study were thus to generate a reference transcriptome for Clione limacina, to expose individuals to CO2 for a period of 3 days, and to explore differential patterns of gene expression. Our Trinity assembly contained 300,994 transcripts of which ~26% could be annotated. In total, only 41 transcripts were differentially expressed following the CO2 treatment, consistent with a limited physiological response of this species to short-term CO2 exposure. The differentially expressed genes identified in our study were largely distinct from those identified in previous studies of thecosome pteropods, although some similar transcripts were identified, suggesting that comparison of these transcriptomes and responses may provide insight into differences in OA responses among phylogenetically and functionally distinct molluscan lineages. A. Thabet is grateful for a fellowship from the Egyptian Culture and Education Bureau and for mentoring from Drs. M.M. Sarhan and M.M. Fouda. Funding for this research was provided by a National Science Foundation grant to Lawson, Maas, and Tarrant (OCE-1316040). |
format |
Report |
author |
Thabet, Ali A. Maas, Amy E. Saber, Samy A. Tarrant, Ann M. |
author_facet |
Thabet, Ali A. Maas, Amy E. Saber, Samy A. Tarrant, Ann M. |
author_sort |
Thabet, Ali A. |
title |
Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure |
title_short |
Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure |
title_full |
Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure |
title_fullStr |
Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure |
title_full_unstemmed |
Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure |
title_sort |
assembly of a reference transcriptome for the gymnosome pteropod clione limacina and profiling responses to short-term co2 exposure |
publishDate |
2017 |
url |
https://hdl.handle.net/1912/9253 |
genre |
Clione limacina Ocean acidification |
genre_facet |
Clione limacina Ocean acidification |
op_relation |
https://doi.org/10.1016/j.margen.2017.03.003 https://hdl.handle.net/1912/9253 |
op_doi |
https://doi.org/10.1016/j.margen.2017.03.003 |
container_title |
Marine Genomics |
container_volume |
34 |
container_start_page |
39 |
op_container_end_page |
45 |
_version_ |
1766391170302214144 |