Evidence of concurrent local adaptation and high phenotypic plasticity in a polar microeukaryote

• Published in: Environmental Microbiology
• Main Authors: Rengefors, Karin, Logares, Ramiro, Laybourn-Parry, Johanna, Gast, Rebecca J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: John Wiley & Sons 2014
• Subjects: Antarctic; Cierva; Antarc*
• Online Access: https://hdl.handle.net/1912/7298

© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Environmental Microbiology 17 (2015): 1510–1519, doi:10.1111/1462-2920.12571. Here we investigated whether there is evidence of local adaptation in strains of an ancestrally marine dinoflagellate to the lacustrine environment they now inhabit (optimal genotypes) and/or if they have evolved phenotypic plasticity (a range of phenotypes). Eleven strains of Polarella glacialis were isolated and cultured from three different environments: the polar seas, a hyposaline and a hypersaline Antarctic lake. Local adaptation was tested by comparing growth rates of lacustrine and marine strains at their own and reciprocal site conditions. To determine phenotypic plasticity, we measured the reaction norm for salinity. We found evidence of both, limited local adaptation and higher phenotypic plasticity in lacustrine strains when compared with marine ancestors. At extreme high salinities, local lake strains outperformed other strains, and at extreme low salinities, strains from the hyposaline lake outperformed all other strains. The data suggest that lake populations may have evolved higher phenotypic plasticity in the lake habitats compared with the sea, presumably due to the high temporal variability in salinity in the lacustrine systems. Moreover, the interval of salinity tolerance differed between strains from the hyposaline and hypersaline lakes, indicating local adaptation promoted by different salinity. This work was supported by a grant from the Australian Antarctic Research Assessment Committee to J.L-P and KR and by The Swedish Research Council (621-2009-5324) to KR. RL has been financed by a Marie Curie Intra-European Fellowship (PIEF-GA-2009–235365, EU) and a Juan de la Cierva fellowship (JCI-2010–06594, Ministry of Science and Innovation, Spain).