Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula
© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 5 (2014): 647, doi:10.3389/fmicb.2014.00647. The Southern Ocean is currently subject to intense investigations, mainly rel...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media
2014
|
Subjects: | |
Online Access: | https://hdl.handle.net/1912/7133 |
id |
ftwhoas:oai:darchive.mblwhoilibrary.org:1912/7133 |
---|---|
record_format |
openpolar |
spelling |
ftwhoas:oai:darchive.mblwhoilibrary.org:1912/7133 2023-05-15T13:53:15+02:00 Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula Signori, Camila N. Thomas, François Enrich-Prast, Alex Pollery, Ricardo C. G. Sievert, Stefan M. 2014-12-16 application/pdf application/msword application/postscript https://hdl.handle.net/1912/7133 en_US eng Frontiers Media https://doi.org/10.3389/fmicb.2014.00647 Frontiers in Microbiology 5 (2014): 647 https://hdl.handle.net/1912/7133 doi:10.3389/fmicb.2014.00647 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY Frontiers in Microbiology 5 (2014): 647 doi:10.3389/fmicb.2014.00647 Antarctica Pyrosequencing Microbial community structure Environmental factors Microbial oceanography Climate change Article 2014 ftwhoas https://doi.org/10.3389/fmicb.2014.00647 2022-05-28T22:59:16Z © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 5 (2014): 647, doi:10.3389/fmicb.2014.00647. The Southern Ocean is currently subject to intense investigations, mainly related to its importance for global biogeochemical cycles and its alarming rate of warming in response to climate change. Microbes play an essential role in the functioning of this ecosystem and are the main drivers of the biogeochemical cycling of elements. Yet, the diversity and abundance of microorganisms in this system remain poorly studied, in particular with regards to changes along environmental gradients. Here, we used amplicon sequencing of 16S rRNA gene tags using primers covering both Bacteria and Archaea to assess the composition and diversity of the microbial communities from four sampling depths (surface, the maximum and minimum of the oxygen concentration, and near the seafloor) at 10 oceanographic stations located in Bransfield Strait [northwest of the Antarctic Peninsula (AP)] and near the sea ice edge (north of the AP). Samples collected near the seafloor and at the oxygen minimum exhibited a higher diversity than those from the surface and oxygen maximum for both bacterial and archaeal communities. The main taxonomic groups identified below 100 m were Thaumarchaeota, Euryarchaeota and Proteobacteria (Gamma-, Delta-, Beta-, and Alphaproteobacteria), whereas in the mixed layer above 100 m Bacteroidetes and Proteobacteria (mainly Alpha- and Gammaproteobacteria) were found to be dominant. A combination of environmental factors seems to influence the microbial community composition. Our results help to understand how the dynamic seascape of the Southern Ocean shapes the microbial community composition and set a baseline for upcoming studies to evaluate the response of this ecosystem to future changes. This work was supported by the Brazilian National Counsel of Technological and Scientific Development ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Bransfield Strait Sea ice Southern Ocean Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Antarctic Southern Ocean The Antarctic Antarctic Peninsula Bransfield Strait Frontiers in Microbiology 5 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
English |
topic |
Antarctica Pyrosequencing Microbial community structure Environmental factors Microbial oceanography Climate change |
spellingShingle |
Antarctica Pyrosequencing Microbial community structure Environmental factors Microbial oceanography Climate change Signori, Camila N. Thomas, François Enrich-Prast, Alex Pollery, Ricardo C. G. Sievert, Stefan M. Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
topic_facet |
Antarctica Pyrosequencing Microbial community structure Environmental factors Microbial oceanography Climate change |
description |
© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 5 (2014): 647, doi:10.3389/fmicb.2014.00647. The Southern Ocean is currently subject to intense investigations, mainly related to its importance for global biogeochemical cycles and its alarming rate of warming in response to climate change. Microbes play an essential role in the functioning of this ecosystem and are the main drivers of the biogeochemical cycling of elements. Yet, the diversity and abundance of microorganisms in this system remain poorly studied, in particular with regards to changes along environmental gradients. Here, we used amplicon sequencing of 16S rRNA gene tags using primers covering both Bacteria and Archaea to assess the composition and diversity of the microbial communities from four sampling depths (surface, the maximum and minimum of the oxygen concentration, and near the seafloor) at 10 oceanographic stations located in Bransfield Strait [northwest of the Antarctic Peninsula (AP)] and near the sea ice edge (north of the AP). Samples collected near the seafloor and at the oxygen minimum exhibited a higher diversity than those from the surface and oxygen maximum for both bacterial and archaeal communities. The main taxonomic groups identified below 100 m were Thaumarchaeota, Euryarchaeota and Proteobacteria (Gamma-, Delta-, Beta-, and Alphaproteobacteria), whereas in the mixed layer above 100 m Bacteroidetes and Proteobacteria (mainly Alpha- and Gammaproteobacteria) were found to be dominant. A combination of environmental factors seems to influence the microbial community composition. Our results help to understand how the dynamic seascape of the Southern Ocean shapes the microbial community composition and set a baseline for upcoming studies to evaluate the response of this ecosystem to future changes. This work was supported by the Brazilian National Counsel of Technological and Scientific Development ... |
format |
Article in Journal/Newspaper |
author |
Signori, Camila N. Thomas, François Enrich-Prast, Alex Pollery, Ricardo C. G. Sievert, Stefan M. |
author_facet |
Signori, Camila N. Thomas, François Enrich-Prast, Alex Pollery, Ricardo C. G. Sievert, Stefan M. |
author_sort |
Signori, Camila N. |
title |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_short |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_full |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_fullStr |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_full_unstemmed |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_sort |
microbial diversity and community structure across environmental gradients in bransfield strait, western antarctic peninsula |
publisher |
Frontiers Media |
publishDate |
2014 |
url |
https://hdl.handle.net/1912/7133 |
geographic |
Antarctic Southern Ocean The Antarctic Antarctic Peninsula Bransfield Strait |
geographic_facet |
Antarctic Southern Ocean The Antarctic Antarctic Peninsula Bransfield Strait |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Bransfield Strait Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Bransfield Strait Sea ice Southern Ocean |
op_source |
Frontiers in Microbiology 5 (2014): 647 doi:10.3389/fmicb.2014.00647 |
op_relation |
https://doi.org/10.3389/fmicb.2014.00647 Frontiers in Microbiology 5 (2014): 647 https://hdl.handle.net/1912/7133 doi:10.3389/fmicb.2014.00647 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2014.00647 |
container_title |
Frontiers in Microbiology |
container_volume |
5 |
_version_ |
1766258254442135552 |