Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition
© The Author(s), 2011. This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums. The definitive version was published in Frontiers in Microbiology 2 (2011): 160, doi:10.3389/fmicb.20...
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/6998 2023-05-15T13:53:15+02:00 Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition Bertrand, Erin M. Saito, Mak A. Lee, Peter A. Dunbar, Robert B. Sedwick, Peter N. DiTullio, Giacomo R. 2011-08-15 application/pdf https://hdl.handle.net/1912/6998 en_US eng Frontiers Media https://doi.org/10.3389/fmicb.2011.00160 Frontiers in Microbiology 2 (2011): 160 https://hdl.handle.net/1912/6998 doi:10.3389/fmicb.2011.00160 Frontiers in Microbiology 2 (2011): 160 doi:10.3389/fmicb.2011.00160 Iron limitation Vitamin B12 Ross Sea Colimitation Bacteria Phytoplankton Iron fertilization Article 2011 ftwhoas https://doi.org/10.3389/fmicb.2011.00160 2022-05-28T22:59:14Z © The Author(s), 2011. This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums. The definitive version was published in Frontiers in Microbiology 2 (2011): 160, doi:10.3389/fmicb.2011.00160. The Ross Sea is home to some of the largest phytoplankton blooms in the Southern Ocean. Primary production in this system has previously been shown to be iron limited in the summer and periodically iron and vitamin B12 colimited. In this study, we examined trace metal limitation of biological activity in the Ross Sea in the austral spring and considered possible implications for vitamin B12 nutrition. Bottle incubation experiments demonstrated that iron limited phytoplankton growth in the austral spring while B12, cobalt, and zinc did not. This is the first demonstration of iron limitation in a Phaeocystis antarctica-dominated, early season Ross Sea phytoplankton community. The lack of B12 limitation in this location is consistent with previous Ross Sea studies in the austral summer, wherein vitamin additions did not stimulate P. antarctica growth and B12 was limiting only when bacterial abundance was low. Bottle incubation experiments and a bacterial regrowth experiment also revealed that iron addition directly enhanced bacterial growth. B12 uptake measurements in natural water samples and in an iron fertilized bottle incubation demonstrated that bacteria serve not only as a source for vitamin B12, but also as a significant sink, and that iron additions enhanced B12 uptake rates in phytoplankton but not bacteria. Additionally, vitamin uptake rates did not become saturated upon the addition of up to 95 pM B12. A rapid B12 uptake rate was observed after 13 min, which then decreased to a slower constant uptake rate over the next 52 h. Results from this study highlight the importance of iron availability in limiting early season Ross Sea phytoplankton growth and suggest that rates of vitamin B12 production ... Article in Journal/Newspaper Antarc* Antarctica Ross Sea Southern Ocean Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Southern Ocean Austral Ross Sea Frontiers in Microbiology 2 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
English |
topic |
Iron limitation Vitamin B12 Ross Sea Colimitation Bacteria Phytoplankton Iron fertilization |
spellingShingle |
Iron limitation Vitamin B12 Ross Sea Colimitation Bacteria Phytoplankton Iron fertilization Bertrand, Erin M. Saito, Mak A. Lee, Peter A. Dunbar, Robert B. Sedwick, Peter N. DiTullio, Giacomo R. Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition |
topic_facet |
Iron limitation Vitamin B12 Ross Sea Colimitation Bacteria Phytoplankton Iron fertilization |
description |
© The Author(s), 2011. This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums. The definitive version was published in Frontiers in Microbiology 2 (2011): 160, doi:10.3389/fmicb.2011.00160. The Ross Sea is home to some of the largest phytoplankton blooms in the Southern Ocean. Primary production in this system has previously been shown to be iron limited in the summer and periodically iron and vitamin B12 colimited. In this study, we examined trace metal limitation of biological activity in the Ross Sea in the austral spring and considered possible implications for vitamin B12 nutrition. Bottle incubation experiments demonstrated that iron limited phytoplankton growth in the austral spring while B12, cobalt, and zinc did not. This is the first demonstration of iron limitation in a Phaeocystis antarctica-dominated, early season Ross Sea phytoplankton community. The lack of B12 limitation in this location is consistent with previous Ross Sea studies in the austral summer, wherein vitamin additions did not stimulate P. antarctica growth and B12 was limiting only when bacterial abundance was low. Bottle incubation experiments and a bacterial regrowth experiment also revealed that iron addition directly enhanced bacterial growth. B12 uptake measurements in natural water samples and in an iron fertilized bottle incubation demonstrated that bacteria serve not only as a source for vitamin B12, but also as a significant sink, and that iron additions enhanced B12 uptake rates in phytoplankton but not bacteria. Additionally, vitamin uptake rates did not become saturated upon the addition of up to 95 pM B12. A rapid B12 uptake rate was observed after 13 min, which then decreased to a slower constant uptake rate over the next 52 h. Results from this study highlight the importance of iron availability in limiting early season Ross Sea phytoplankton growth and suggest that rates of vitamin B12 production ... |
format |
Article in Journal/Newspaper |
author |
Bertrand, Erin M. Saito, Mak A. Lee, Peter A. Dunbar, Robert B. Sedwick, Peter N. DiTullio, Giacomo R. |
author_facet |
Bertrand, Erin M. Saito, Mak A. Lee, Peter A. Dunbar, Robert B. Sedwick, Peter N. DiTullio, Giacomo R. |
author_sort |
Bertrand, Erin M. |
title |
Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition |
title_short |
Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition |
title_full |
Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition |
title_fullStr |
Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition |
title_full_unstemmed |
Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea : implications for vitamin B12 nutrition |
title_sort |
iron limitation of a springtime bacterial and phytoplankton community in the ross sea : implications for vitamin b12 nutrition |
publisher |
Frontiers Media |
publishDate |
2011 |
url |
https://hdl.handle.net/1912/6998 |
geographic |
Southern Ocean Austral Ross Sea |
geographic_facet |
Southern Ocean Austral Ross Sea |
genre |
Antarc* Antarctica Ross Sea Southern Ocean |
genre_facet |
Antarc* Antarctica Ross Sea Southern Ocean |
op_source |
Frontiers in Microbiology 2 (2011): 160 doi:10.3389/fmicb.2011.00160 |
op_relation |
https://doi.org/10.3389/fmicb.2011.00160 Frontiers in Microbiology 2 (2011): 160 https://hdl.handle.net/1912/6998 doi:10.3389/fmicb.2011.00160 |
op_doi |
https://doi.org/10.3389/fmicb.2011.00160 |
container_title |
Frontiers in Microbiology |
container_volume |
2 |
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1766258253491077120 |