Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica
Author Posting. © Marine Biological Laboratory, 2014. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 227 (2014): 175-190. We report an in-depth survey of next-generation D...
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/6965 2023-05-15T13:53:15+02:00 Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica Xu, Yuan Vick-Majors, Trista Morgan-Kiss, Rachael Priscu, John C. Amaral-Zettler, Linda A. 2014-10-01 application/pdf https://hdl.handle.net/1912/6965 en_US eng Marine Biological Laboratory https://doi.org/10.1086/BBLv227n2p175 Biological Bulletin 227 (2014): 175-190 https://hdl.handle.net/1912/6965 doi:10.1086/BBLv227n2p175 Biological Bulletin 227 (2014): 175-190 doi:10.1086/BBLv227n2p175 Article 2014 ftwhoas https://doi.org/10.1086/BBLv227n2p175 2022-05-28T22:59:12Z Author Posting. © Marine Biological Laboratory, 2014. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 227 (2014): 175-190. We report an in-depth survey of next-generation DNA sequencing of ciliate diversity and community structure in two permanently ice-covered McMurdo Dry Valley lakes during the austral summer and autumn (November 2007 and March 2008). We tested hypotheses on the relationship between species richness and environmental conditions including environmental extremes, nutrient status, and day length. On the basis of the unique environment that exists in these high-latitude lakes, we expected that novel taxa would be present. Alpha diversity analyses showed that extreme conditions—that is, high salinity, low oxygen, and extreme changes in day length—did not impact ciliate richness; however, ciliate richness was 30% higher in samples with higher dissolved organic matter. Beta diversity analyses revealed that ciliate communities clustered by dissolved oxygen, depth, and salinity, but not by season (i.e., day length). The permutational analysis of variance test indicated that depth, dissolved oxygen, and salinity had significant influences on the ciliate community for the abundance matrices of resampled data, while lake and season were not significant. This result suggests that the vertical trends in dissolved oxygen concentration and salinity may play a critical role in structuring ciliate communities. A PCR-based strategy capitalizing on divergent eukaryotic V9 hypervariable region ribosomal RNA gene targets unveiled two new genera in these lakes. A novel taxon belonging to an unknown class most closely related to Cryptocaryon irritans was also inferred from separate gene phylogenies. Funding was provided by NSF DEB-0717390 to Linda Amaral-Zettler (MIRADA-LTERS); OPP-1115245, OPP-0838933, OPP-1027284, and OPP-0839075 to John C. Priscu; and OPP-0631659 and OPP-1056396 to ... Article in Journal/Newspaper Antarc* Antarctica McMurdo Dry Valleys Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Austral McMurdo Dry Valleys The Biological Bulletin 227 2 175 190 |
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Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
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ftwhoas |
language |
English |
description |
Author Posting. © Marine Biological Laboratory, 2014. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 227 (2014): 175-190. We report an in-depth survey of next-generation DNA sequencing of ciliate diversity and community structure in two permanently ice-covered McMurdo Dry Valley lakes during the austral summer and autumn (November 2007 and March 2008). We tested hypotheses on the relationship between species richness and environmental conditions including environmental extremes, nutrient status, and day length. On the basis of the unique environment that exists in these high-latitude lakes, we expected that novel taxa would be present. Alpha diversity analyses showed that extreme conditions—that is, high salinity, low oxygen, and extreme changes in day length—did not impact ciliate richness; however, ciliate richness was 30% higher in samples with higher dissolved organic matter. Beta diversity analyses revealed that ciliate communities clustered by dissolved oxygen, depth, and salinity, but not by season (i.e., day length). The permutational analysis of variance test indicated that depth, dissolved oxygen, and salinity had significant influences on the ciliate community for the abundance matrices of resampled data, while lake and season were not significant. This result suggests that the vertical trends in dissolved oxygen concentration and salinity may play a critical role in structuring ciliate communities. A PCR-based strategy capitalizing on divergent eukaryotic V9 hypervariable region ribosomal RNA gene targets unveiled two new genera in these lakes. A novel taxon belonging to an unknown class most closely related to Cryptocaryon irritans was also inferred from separate gene phylogenies. Funding was provided by NSF DEB-0717390 to Linda Amaral-Zettler (MIRADA-LTERS); OPP-1115245, OPP-0838933, OPP-1027284, and OPP-0839075 to John C. Priscu; and OPP-0631659 and OPP-1056396 to ... |
format |
Article in Journal/Newspaper |
author |
Xu, Yuan Vick-Majors, Trista Morgan-Kiss, Rachael Priscu, John C. Amaral-Zettler, Linda A. |
spellingShingle |
Xu, Yuan Vick-Majors, Trista Morgan-Kiss, Rachael Priscu, John C. Amaral-Zettler, Linda A. Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica |
author_facet |
Xu, Yuan Vick-Majors, Trista Morgan-Kiss, Rachael Priscu, John C. Amaral-Zettler, Linda A. |
author_sort |
Xu, Yuan |
title |
Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica |
title_short |
Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica |
title_full |
Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica |
title_fullStr |
Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica |
title_full_unstemmed |
Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica |
title_sort |
ciliate diversity, community structure, and novel taxa in lakes of the mcmurdo dry valleys, antarctica |
publisher |
Marine Biological Laboratory |
publishDate |
2014 |
url |
https://hdl.handle.net/1912/6965 |
geographic |
Austral McMurdo Dry Valleys |
geographic_facet |
Austral McMurdo Dry Valleys |
genre |
Antarc* Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctica McMurdo Dry Valleys |
op_source |
Biological Bulletin 227 (2014): 175-190 doi:10.1086/BBLv227n2p175 |
op_relation |
https://doi.org/10.1086/BBLv227n2p175 Biological Bulletin 227 (2014): 175-190 https://hdl.handle.net/1912/6965 doi:10.1086/BBLv227n2p175 |
op_doi |
https://doi.org/10.1086/BBLv227n2p175 |
container_title |
The Biological Bulletin |
container_volume |
227 |
container_issue |
2 |
container_start_page |
175 |
op_container_end_page |
190 |
_version_ |
1766258252897583104 |