Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments
Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 111 (2006): G03013, doi:10.1029/2005JG000121. The 18S ribosoma...
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/3542 2023-05-15T13:53:14+02:00 Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments D'Andrea, William J. Lage, Melissa Martiny, Jennifer B. H. Laatsch, Abby D. Amaral-Zettler, Linda A. Sogin, Mitchell L. Huang, Yongsong 2006-09-19 application/pdf https://hdl.handle.net/1912/3542 en_US eng American Geophysical Union https://doi.org/10.1029/2005JG000121 Journal of Geophysical Research 111 (2006): G03013 https://hdl.handle.net/1912/3542 doi:10.1029/2005JG000121 Journal of Geophysical Research 111 (2006): G03013 doi:10.1029/2005JG000121 Alkenone RDNA Haptophyte Article 2006 ftwhoas https://doi.org/10.1029/2005JG000121 2022-05-28T22:58:00Z Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 111 (2006): G03013, doi:10.1029/2005JG000121. The 18S ribosomal DNA (rDNA) sequences of haptophyte algae were successfully amplified using the polymerase chain reaction (PCR) from water filtrate, surface sediments, and a late-Holocene sediment sample (∼1000 years old) from a group of lakes in the Søndre Strømfjord region of west Greenland. The DNA of the algal primary producer is extremely well preserved in the laminated lake sediments which have been deposited in cold (1°–2°C), anoxic, and sulphidic bottom water. Phylogenetic analyses of the Greenland haptophyte rDNA sequences suggest that alkenones in the Greenland lake sediments are produced by haptophyte algae of the class Prymnesiophyceae. The 18S rDNA sequences from the Greenland samples cluster within a distinct phylotype, differing from both marine haptophytes and from those reported previously from Ace Lake, Antarctica. The similarity of haptophyte rDNA sequences among all samples in this study suggests a single alkenone-based temperature calibration may be applied to these lakes for at least the past 1000 years. These sedimentary archives hold great promise for high-resolution, alkenone-based paleotemperature reconstruction of southern west Greenland, a region sensitive to atmospheric-oceanic climate phenomena such as the North Atlantic Oscillation (NAO). This work was supported by grants from the National Science Foundation (NSF0081478, 0318050, 0318123, 0402383, 0520718), NASA (NAG5-10665, NNG04GJ34G) and the American Chemical Society, Petroleum Research Fund (ACS-PRF38878-AC2) to Y. Huang. Article in Journal/Newspaper Antarc* Antarctica Greenland North Atlantic North Atlantic oscillation Søndre strømfjord Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Greenland Journal of Geophysical Research 111 G3 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
English |
topic |
Alkenone RDNA Haptophyte |
spellingShingle |
Alkenone RDNA Haptophyte D'Andrea, William J. Lage, Melissa Martiny, Jennifer B. H. Laatsch, Abby D. Amaral-Zettler, Linda A. Sogin, Mitchell L. Huang, Yongsong Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments |
topic_facet |
Alkenone RDNA Haptophyte |
description |
Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 111 (2006): G03013, doi:10.1029/2005JG000121. The 18S ribosomal DNA (rDNA) sequences of haptophyte algae were successfully amplified using the polymerase chain reaction (PCR) from water filtrate, surface sediments, and a late-Holocene sediment sample (∼1000 years old) from a group of lakes in the Søndre Strømfjord region of west Greenland. The DNA of the algal primary producer is extremely well preserved in the laminated lake sediments which have been deposited in cold (1°–2°C), anoxic, and sulphidic bottom water. Phylogenetic analyses of the Greenland haptophyte rDNA sequences suggest that alkenones in the Greenland lake sediments are produced by haptophyte algae of the class Prymnesiophyceae. The 18S rDNA sequences from the Greenland samples cluster within a distinct phylotype, differing from both marine haptophytes and from those reported previously from Ace Lake, Antarctica. The similarity of haptophyte rDNA sequences among all samples in this study suggests a single alkenone-based temperature calibration may be applied to these lakes for at least the past 1000 years. These sedimentary archives hold great promise for high-resolution, alkenone-based paleotemperature reconstruction of southern west Greenland, a region sensitive to atmospheric-oceanic climate phenomena such as the North Atlantic Oscillation (NAO). This work was supported by grants from the National Science Foundation (NSF0081478, 0318050, 0318123, 0402383, 0520718), NASA (NAG5-10665, NNG04GJ34G) and the American Chemical Society, Petroleum Research Fund (ACS-PRF38878-AC2) to Y. Huang. |
format |
Article in Journal/Newspaper |
author |
D'Andrea, William J. Lage, Melissa Martiny, Jennifer B. H. Laatsch, Abby D. Amaral-Zettler, Linda A. Sogin, Mitchell L. Huang, Yongsong |
author_facet |
D'Andrea, William J. Lage, Melissa Martiny, Jennifer B. H. Laatsch, Abby D. Amaral-Zettler, Linda A. Sogin, Mitchell L. Huang, Yongsong |
author_sort |
D'Andrea, William J. |
title |
Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments |
title_short |
Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments |
title_full |
Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments |
title_fullStr |
Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments |
title_full_unstemmed |
Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments |
title_sort |
alkenone producers inferred from well-preserved 18s rdna in greenland lake sediments |
publisher |
American Geophysical Union |
publishDate |
2006 |
url |
https://hdl.handle.net/1912/3542 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake Greenland |
geographic_facet |
Ace Lake Greenland |
genre |
Antarc* Antarctica Greenland North Atlantic North Atlantic oscillation Søndre strømfjord |
genre_facet |
Antarc* Antarctica Greenland North Atlantic North Atlantic oscillation Søndre strømfjord |
op_source |
Journal of Geophysical Research 111 (2006): G03013 doi:10.1029/2005JG000121 |
op_relation |
https://doi.org/10.1029/2005JG000121 Journal of Geophysical Research 111 (2006): G03013 https://hdl.handle.net/1912/3542 doi:10.1029/2005JG000121 |
op_doi |
https://doi.org/10.1029/2005JG000121 |
container_title |
Journal of Geophysical Research |
container_volume |
111 |
container_issue |
G3 |
_version_ |
1766258226854100992 |