COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf
© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. COI metabarcoding of zooplankton species diversit...
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Online Access: | https://hdl.handle.net/1912/29190 |
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/29190 2023-05-15T17:36:05+02:00 COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf Bucklin, Ann Batta-Lona, Paola G. Questel, Jennifer M. Wiebe, Peter Richardson, David E. Copley, Nancy O'Brien, Todd D. 2022-04-22 https://hdl.handle.net/1912/29190 unknown Frontiers Media https://doi.org/10.3389/fmars.2022.867893 Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. (2022). COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic Continental Shelf. Frontiers in Marine Science, 9, 867893. https://hdl.handle.net/1912/29190 doi:10.3389/fmars.2022.867893 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. (2022). COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic Continental Shelf. Frontiers in Marine Science, 9, 867893. doi:10.3389/fmars.2022.867893 zooplankton metabarcoding cytochrome oxidase I species diversity ecosystem monitoring Northwest Atlantic continental shelf Article 2022 ftwhoas https://doi.org/10.3389/fmars.2022.867893 2022-08-06T22:56:43Z © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf. Frontiers in Marine Science, 9, (2022): 867893, https://doi.org/10.3389/fmars.2022.867893. Marine zooplankton are rapid-responders and useful indicators of environmental variability and climate change impacts on pelagic ecosystems on time scales ranging from seasons to years to decades. The systematic complexity and taxonomic diversity of the zooplankton assemblage has presented significant challenges for routine morphological (microscopic) identification of species in samples collected during ecosystem monitoring and fisheries management surveys. Metabarcoding using the mitochondrial Cytochrome Oxidase I (COI) gene region has shown promise for detecting and identifying species of some – but not all – taxonomic groups in samples of marine zooplankton. This study examined species diversity of zooplankton on the Northwest Atlantic Continental Shelf using 27 samples collected in 2002-2012 from the Gulf of Maine, Georges Bank, and Mid-Atlantic Bight during Ecosystem Monitoring (EcoMon) Surveys by the NOAA NMFS Northeast Fisheries Science Center. COI metabarcodes were identified using the MetaZooGene Barcode Atlas and Database (https://metazoogene.org/MZGdb) specific to the North Atlantic Ocean. A total of 181 species across 23 taxonomic groups were detected, including a number of sibling and cryptic species that were not discriminated by morphological taxonomic analysis of EcoMon samples. In all, 67 species of 15 taxonomic groups had ≥ 50 COI sequences; 23 species had >1,000 COI sequences. Comparative analysis of molecular and morphological data showed significant correlations between COI sequence numbers and microscopic counts for 5 of 6 taxonomic ... Article in Journal/Newspaper North Atlantic Northwest Atlantic Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Batta ENVELOPE(29.816,29.816,69.797,69.797) Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
unknown |
topic |
zooplankton metabarcoding cytochrome oxidase I species diversity ecosystem monitoring Northwest Atlantic continental shelf |
spellingShingle |
zooplankton metabarcoding cytochrome oxidase I species diversity ecosystem monitoring Northwest Atlantic continental shelf Bucklin, Ann Batta-Lona, Paola G. Questel, Jennifer M. Wiebe, Peter Richardson, David E. Copley, Nancy O'Brien, Todd D. COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf |
topic_facet |
zooplankton metabarcoding cytochrome oxidase I species diversity ecosystem monitoring Northwest Atlantic continental shelf |
description |
© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf. Frontiers in Marine Science, 9, (2022): 867893, https://doi.org/10.3389/fmars.2022.867893. Marine zooplankton are rapid-responders and useful indicators of environmental variability and climate change impacts on pelagic ecosystems on time scales ranging from seasons to years to decades. The systematic complexity and taxonomic diversity of the zooplankton assemblage has presented significant challenges for routine morphological (microscopic) identification of species in samples collected during ecosystem monitoring and fisheries management surveys. Metabarcoding using the mitochondrial Cytochrome Oxidase I (COI) gene region has shown promise for detecting and identifying species of some – but not all – taxonomic groups in samples of marine zooplankton. This study examined species diversity of zooplankton on the Northwest Atlantic Continental Shelf using 27 samples collected in 2002-2012 from the Gulf of Maine, Georges Bank, and Mid-Atlantic Bight during Ecosystem Monitoring (EcoMon) Surveys by the NOAA NMFS Northeast Fisheries Science Center. COI metabarcodes were identified using the MetaZooGene Barcode Atlas and Database (https://metazoogene.org/MZGdb) specific to the North Atlantic Ocean. A total of 181 species across 23 taxonomic groups were detected, including a number of sibling and cryptic species that were not discriminated by morphological taxonomic analysis of EcoMon samples. In all, 67 species of 15 taxonomic groups had ≥ 50 COI sequences; 23 species had >1,000 COI sequences. Comparative analysis of molecular and morphological data showed significant correlations between COI sequence numbers and microscopic counts for 5 of 6 taxonomic ... |
format |
Article in Journal/Newspaper |
author |
Bucklin, Ann Batta-Lona, Paola G. Questel, Jennifer M. Wiebe, Peter Richardson, David E. Copley, Nancy O'Brien, Todd D. |
author_facet |
Bucklin, Ann Batta-Lona, Paola G. Questel, Jennifer M. Wiebe, Peter Richardson, David E. Copley, Nancy O'Brien, Todd D. |
author_sort |
Bucklin, Ann |
title |
COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf |
title_short |
COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf |
title_full |
COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf |
title_fullStr |
COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf |
title_full_unstemmed |
COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf |
title_sort |
coi metabarcoding of zooplankton species diversity for time-series monitoring of the nw atlantic continental shelf |
publisher |
Frontiers Media |
publishDate |
2022 |
url |
https://hdl.handle.net/1912/29190 |
long_lat |
ENVELOPE(29.816,29.816,69.797,69.797) |
geographic |
Batta |
geographic_facet |
Batta |
genre |
North Atlantic Northwest Atlantic |
genre_facet |
North Atlantic Northwest Atlantic |
op_source |
Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. (2022). COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic Continental Shelf. Frontiers in Marine Science, 9, 867893. doi:10.3389/fmars.2022.867893 |
op_relation |
https://doi.org/10.3389/fmars.2022.867893 Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. (2022). COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic Continental Shelf. Frontiers in Marine Science, 9, 867893. https://hdl.handle.net/1912/29190 doi:10.3389/fmars.2022.867893 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2022.867893 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1766135452831580160 |