COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf

• Published in: Frontiers in Marine Science
• Main Authors: Bucklin, Ann, Batta-Lona, Paola G., Questel, Jennifer M., Wiebe, Peter, Richardson, David E., Copley, Nancy, O'Brien, Todd D.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Frontiers Media 2022
• Subjects: zooplankton; metabarcoding; cytochrome oxidase I; species diversity; ecosystem monitoring; Northwest Atlantic continental shelf; Batta; North Atlantic; Northwest Atlantic
• Online Access: https://hdl.handle.net/1912/29190

© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bucklin, A., Batta-Lona, P., Questel, J., Wiebe, P., Richardson, D., Copley, N., & O’Brien, T. COI metabarcoding of zooplankton species diversity for time-series monitoring of the NW Atlantic continental shelf. Frontiers in Marine Science, 9, (2022): 867893, https://doi.org/10.3389/fmars.2022.867893. Marine zooplankton are rapid-responders and useful indicators of environmental variability and climate change impacts on pelagic ecosystems on time scales ranging from seasons to years to decades. The systematic complexity and taxonomic diversity of the zooplankton assemblage has presented significant challenges for routine morphological (microscopic) identification of species in samples collected during ecosystem monitoring and fisheries management surveys. Metabarcoding using the mitochondrial Cytochrome Oxidase I (COI) gene region has shown promise for detecting and identifying species of some – but not all – taxonomic groups in samples of marine zooplankton. This study examined species diversity of zooplankton on the Northwest Atlantic Continental Shelf using 27 samples collected in 2002-2012 from the Gulf of Maine, Georges Bank, and Mid-Atlantic Bight during Ecosystem Monitoring (EcoMon) Surveys by the NOAA NMFS Northeast Fisheries Science Center. COI metabarcodes were identified using the MetaZooGene Barcode Atlas and Database (https://metazoogene.org/MZGdb) specific to the North Atlantic Ocean. A total of 181 species across 23 taxonomic groups were detected, including a number of sibling and cryptic species that were not discriminated by morphological taxonomic analysis of EcoMon samples. In all, 67 species of 15 taxonomic groups had ≥ 50 COI sequences; 23 species had >1,000 COI sequences. Comparative analysis of molecular and morphological data showed significant correlations between COI sequence numbers and microscopic counts for 5 of 6 taxonomic ...