Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro
© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. Polycyclic aromatic hydrocarbons modu...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Elsevier
2021
|
Subjects: | |
Online Access: | https://hdl.handle.net/1912/27708 |
id |
ftwhoas:oai:darchive.mblwhoilibrary.org:1912/27708 |
---|---|
record_format |
openpolar |
spelling |
ftwhoas:oai:darchive.mblwhoilibrary.org:1912/27708 2023-05-15T15:27:00+02:00 Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro Goksøyr, Siri Ofsthus Goldstone, Jared V. Lille-Langoy, Roger Lock, Erik-Jan Olsvik, Pål A. Goksøyr, Anders Karlsen, Odd André 2021-07-16 https://hdl.handle.net/1912/27708 unknown Elsevier https://doi.org/10.1016/j.aquatox.2021.105914 Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. (2021). Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro. Aquatic Toxicology, 238, 105914. https://hdl.handle.net/1912/27708 doi:10.1016/j.aquatox.2021.105914 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. (2021). Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro. Aquatic Toxicology, 238, 105914. doi:10.1016/j.aquatox.2021.105914 Environmental toxicology Antagonism PAH Calcitriol Nuclear receptor Potentiation Article 2021 ftwhoas https://doi.org/10.1016/j.aquatox.2021.105914 2022-05-28T23:04:19Z © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro. Aquatic Toxicology, 238, (2021): 105914, https://doi.org/10.1016/j.aquatox.2021.105914. Vitamin D receptor (VDR) mediates the biological function of the steroid hormone calcitriol, which is the metabolically active version of vitamin D. Calcitriol is important for a wide array of physiological functions, including calcium and phosphate homeostasis. In contrast to mammals, which harbor one VDR encoding gene, teleosts possess two orthologous vdr genes encoding Vdr alpha (Vdra) and Vdr beta (Vdrb). Genome mining identified the vdra and vdrb paralogs in the Atlantic cod (Gadus morhua) genome, which were further characterized regarding their phylogeny, tissue-specific expression, and transactivational properties induced by calcitriol. In addition, a selected set of polycyclic aromatic hydrocarbons (PAHs), including naphthalene, phenanthrene, fluorene, pyrene, chrysene, benzo[a]pyrene (BaP), and 7-methylbenzo[a]pyrene, were assessed for their ability to modulate the transcriptional activity of gmVdra and gmVdrb in vitro. Both gmVdra and gmVdrb were activated by calcitriol with similar potencies, but gmVdra produced significantly higher maximal fold activation. Notably, none of the tested PAHs showed agonistic properties towards the Atlantic cod Vdrs. However, binary exposures of calcitriol together with phenanthrene, fluorene, or pyrene, antagonized the activation of gmVdra, while chrysene and BaP significantly potentiated the calcitriol-mediated activity of both receptors. Homology modeling, solvent mapping, and docking analyses complemented the experimental data, and revealed a putative secondary binding site in addition to the ... Article in Journal/Newspaper atlantic cod Gadus morhua Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Karlsen ENVELOPE(-46.000,-46.000,-60.350,-60.350) Aquatic Toxicology 238 105914 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
unknown |
topic |
Environmental toxicology Antagonism PAH Calcitriol Nuclear receptor Potentiation |
spellingShingle |
Environmental toxicology Antagonism PAH Calcitriol Nuclear receptor Potentiation Goksøyr, Siri Ofsthus Goldstone, Jared V. Lille-Langoy, Roger Lock, Erik-Jan Olsvik, Pål A. Goksøyr, Anders Karlsen, Odd André Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro |
topic_facet |
Environmental toxicology Antagonism PAH Calcitriol Nuclear receptor Potentiation |
description |
© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro. Aquatic Toxicology, 238, (2021): 105914, https://doi.org/10.1016/j.aquatox.2021.105914. Vitamin D receptor (VDR) mediates the biological function of the steroid hormone calcitriol, which is the metabolically active version of vitamin D. Calcitriol is important for a wide array of physiological functions, including calcium and phosphate homeostasis. In contrast to mammals, which harbor one VDR encoding gene, teleosts possess two orthologous vdr genes encoding Vdr alpha (Vdra) and Vdr beta (Vdrb). Genome mining identified the vdra and vdrb paralogs in the Atlantic cod (Gadus morhua) genome, which were further characterized regarding their phylogeny, tissue-specific expression, and transactivational properties induced by calcitriol. In addition, a selected set of polycyclic aromatic hydrocarbons (PAHs), including naphthalene, phenanthrene, fluorene, pyrene, chrysene, benzo[a]pyrene (BaP), and 7-methylbenzo[a]pyrene, were assessed for their ability to modulate the transcriptional activity of gmVdra and gmVdrb in vitro. Both gmVdra and gmVdrb were activated by calcitriol with similar potencies, but gmVdra produced significantly higher maximal fold activation. Notably, none of the tested PAHs showed agonistic properties towards the Atlantic cod Vdrs. However, binary exposures of calcitriol together with phenanthrene, fluorene, or pyrene, antagonized the activation of gmVdra, while chrysene and BaP significantly potentiated the calcitriol-mediated activity of both receptors. Homology modeling, solvent mapping, and docking analyses complemented the experimental data, and revealed a putative secondary binding site in addition to the ... |
format |
Article in Journal/Newspaper |
author |
Goksøyr, Siri Ofsthus Goldstone, Jared V. Lille-Langoy, Roger Lock, Erik-Jan Olsvik, Pål A. Goksøyr, Anders Karlsen, Odd André |
author_facet |
Goksøyr, Siri Ofsthus Goldstone, Jared V. Lille-Langoy, Roger Lock, Erik-Jan Olsvik, Pål A. Goksøyr, Anders Karlsen, Odd André |
author_sort |
Goksøyr, Siri Ofsthus |
title |
Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro |
title_short |
Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro |
title_full |
Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro |
title_fullStr |
Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro |
title_full_unstemmed |
Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro |
title_sort |
polycyclic aromatic hydrocarbons modulate the activity of atlantic cod (gadus morhua) vitamin d receptor paralogs in vitro |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://hdl.handle.net/1912/27708 |
long_lat |
ENVELOPE(-46.000,-46.000,-60.350,-60.350) |
geographic |
Karlsen |
geographic_facet |
Karlsen |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. (2021). Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro. Aquatic Toxicology, 238, 105914. doi:10.1016/j.aquatox.2021.105914 |
op_relation |
https://doi.org/10.1016/j.aquatox.2021.105914 Goksoyr, S. O., Goldstone, J., Lille-Langoy, R., Lock, E.-J., Olsvik, P. A., Goksoyr, A., & Karlsen, O. A. (2021). Polycyclic aromatic hydrocarbons modulate the activity of Atlantic cod (Gadus morhua) vitamin D receptor paralogs in vitro. Aquatic Toxicology, 238, 105914. https://hdl.handle.net/1912/27708 doi:10.1016/j.aquatox.2021.105914 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.aquatox.2021.105914 |
container_title |
Aquatic Toxicology |
container_volume |
238 |
container_start_page |
105914 |
_version_ |
1766357467139145728 |