Microbial enzyme activities: peptidase activities of gravity-filtered seawater samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012

Dataset: ARK27-3: GF MCA hydrolysis rates This dataset includes microbial peptidase hydrolysis rates measured on particles collected from gravity filtered seawater. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid –...

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Bibliographic Details
Main Author: Arnosti, Carol
Format: Dataset
Language:English
Published: Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu 2020
Subjects:
Arctic
Arctic Ocean
Greenland
Laptev Sea
Pacific
laptev
Online Access:https://hdl.handle.net/1912/25687
Description
Summary:Dataset: ARK27-3: GF MCA hydrolysis rates This dataset includes microbial peptidase hydrolysis rates measured on particles collected from gravity filtered seawater. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/742967 NSF Division of Ocean Sciences (NSF OCE) OCE-1332881

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