Measuring the Adapted Low Temperature Resilience of Cold Shock Proteins - using Single Molecule Force Spectroscopy and Nuclear Magnetic Resonance

• Main Author: Kendrick, Katherine Ellen
• Format: Thesis
• Language: English
• Published: University of Leeds 2018
• Subjects: Arctic
• Online Access: https://etheses.whiterose.ac.uk/24226/; https://etheses.whiterose.ac.uk/24226/1/Kendrick_KE_Physics_PhD_2018.pdf

Cold Shock Proteins (CSPs) are produced when organisms experience cold-shock, a sudden drop to roughly 7-10 °C below the optimum temperature, and are thought to aid the melting of non-functional RNA secondary structures. For this function CSPs are required to stay exible at temperatures determined by the optimum environment of the organism. While the thermodynamic properties of these proteins vary depending on the optimum temperature of the source organism, their structures are highly conserved, making this a good model system to explore non-structural adaptations to various environments. Single molecule force spectroscopy was used to measure the mechanical properties of the CSP from Bacillus Subtilis while in the functional state, i.e. bound to nucleic acids. This binding was shown to stabilise the protein, without increasing the rigidity, even when the temperature of the complex was reduced. The affinity of the CSP binding to ssDNA was measured and this was compared to the affinity to ssDNA of a CSP from an Arctic soil bacterium, Psychrobacter sp6. The CSP from the organism adapted to colder environments was found to have a lower binding affinity to ssDNA and the binding strength of ssDNA to both CSPs increased with decreasing temperature. When the binding of ssDNA was measured at the respective cold-shock temperatures of each organism the affinities were of the same order of magnitude, suggesting that the strength of this interaction is tuned to the native environment. To explore how functionality is maintained in organisms adapted to extremely cold environments, the dynamics of the CSP from Psychrobacter sp6 was measured at low, physiologically relevant temperatures using a range of NMR techniques. Results showed evidence of slow exchange with a more disordered state at low temperatures together with fast dynamics at the active site. These were not seen in measurements of the CSP from Bacillus Subtilis at low temperatures, indicating they are part of the protein adaptation to cold environments.