The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli
Arabidopsis lyrata ssp. petraea has a fragmented distribution across Northern Eurasia, and its geographically isolated populations experience varying environmental conditions throughout the species’ range. A. l. petraea is consequently an appropriate test system to study the effects of abiotic stres...
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University of Leeds
2010
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Online Access: | https://etheses.whiterose.ac.uk/1448/ https://etheses.whiterose.ac.uk/1448/1/Chloe_Elizabeth_Thompson_PhD_2010.pdf https://etheses.whiterose.ac.uk/1448/2/Appendix_1.pdf https://etheses.whiterose.ac.uk/1448/3/Appendix_2a.pdf https://etheses.whiterose.ac.uk/1448/4/Appendix_2b.pdf https://etheses.whiterose.ac.uk/1448/5/Appendix_2c.pdf https://etheses.whiterose.ac.uk/1448/6/Appendix_2d.pdf https://etheses.whiterose.ac.uk/1448/7/Appendix_2e.pdf |
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ftwhiterose:oai:etheses.whiterose.ac.uk:1448 2023-05-15T16:48:47+02:00 The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli Thompson, Chloe Elizabeth 2010-09 text https://etheses.whiterose.ac.uk/1448/ https://etheses.whiterose.ac.uk/1448/1/Chloe_Elizabeth_Thompson_PhD_2010.pdf https://etheses.whiterose.ac.uk/1448/2/Appendix_1.pdf https://etheses.whiterose.ac.uk/1448/3/Appendix_2a.pdf https://etheses.whiterose.ac.uk/1448/4/Appendix_2b.pdf https://etheses.whiterose.ac.uk/1448/5/Appendix_2c.pdf https://etheses.whiterose.ac.uk/1448/6/Appendix_2d.pdf https://etheses.whiterose.ac.uk/1448/7/Appendix_2e.pdf en eng University of Leeds https://etheses.whiterose.ac.uk/1448/1/Chloe_Elizabeth_Thompson_PhD_2010.pdf https://etheses.whiterose.ac.uk/1448/2/Appendix_1.pdf https://etheses.whiterose.ac.uk/1448/3/Appendix_2a.pdf https://etheses.whiterose.ac.uk/1448/4/Appendix_2b.pdf https://etheses.whiterose.ac.uk/1448/5/Appendix_2c.pdf https://etheses.whiterose.ac.uk/1448/6/Appendix_2d.pdf https://etheses.whiterose.ac.uk/1448/7/Appendix_2e.pdf Thompson, Chloe Elizabeth (2010) The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli. PhD thesis, University of Leeds. cc_by_nc_sa CC-BY-NC-SA Thesis NonPeerReviewed 2010 ftwhiterose 2023-01-30T21:18:03Z Arabidopsis lyrata ssp. petraea has a fragmented distribution across Northern Eurasia, and its geographically isolated populations experience varying environmental conditions throughout the species’ range. A. l. petraea is consequently an appropriate test system to study the effects of abiotic stress on species’ distributions and for investigation of local adaptation. The main objective of this study was to investigate whether or not geographically isolated populations of A. l. petraea differ in their responses to environmental stimuli and thus demonstrate potential for local adaptation. Under controlled environmental conditions, populations from the British Isles and Norway demonstrated the highest average growth rates whilst the lowest rates were observed in Swedish populations. Populations also exhibited significantly altered growth rates under imposed hot and cold stress conditions than in their corresponding no-stress controls. The percentage of plants that flowered was significantly higher in Norwegian populations than for those from Sweden, Iceland, and the British Isles. Molecular techniques were employed to investigate differential gene expression in populations of A. l. petraea from Norway, Sweden, Iceland, and the British Isles, both prior to and following exposure to cold temperature stress. Extent of up-regulation of expression following exposure to chilling differed for a number of genes including CCA1, NAC2, and SKIP16. Microarray analysis identified 207 genes that were differentially expressed between two focal populations, Leitrim (Ireland) and Helin (Norway), irrespective of temperature treatment (P <0.05, fold change >2). Approximately 6% of the A. l. petraea transcriptome was responsive to acclimation whilst expression of 75 genes, many annotated as unknown function, changed following exposure to freezing. The identified phenotypic variation is indicative of local adaptation, and populations from throughout the species’ range may thus harbour unique genetic variation. Further ... Thesis Iceland White Rose eTheses Online (Universities Leeds, Sheffield, York) Helin ENVELOPE(19.017,19.017,74.467,74.467) Norway |
institution |
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collection |
White Rose eTheses Online (Universities Leeds, Sheffield, York) |
op_collection_id |
ftwhiterose |
language |
English |
description |
Arabidopsis lyrata ssp. petraea has a fragmented distribution across Northern Eurasia, and its geographically isolated populations experience varying environmental conditions throughout the species’ range. A. l. petraea is consequently an appropriate test system to study the effects of abiotic stress on species’ distributions and for investigation of local adaptation. The main objective of this study was to investigate whether or not geographically isolated populations of A. l. petraea differ in their responses to environmental stimuli and thus demonstrate potential for local adaptation. Under controlled environmental conditions, populations from the British Isles and Norway demonstrated the highest average growth rates whilst the lowest rates were observed in Swedish populations. Populations also exhibited significantly altered growth rates under imposed hot and cold stress conditions than in their corresponding no-stress controls. The percentage of plants that flowered was significantly higher in Norwegian populations than for those from Sweden, Iceland, and the British Isles. Molecular techniques were employed to investigate differential gene expression in populations of A. l. petraea from Norway, Sweden, Iceland, and the British Isles, both prior to and following exposure to cold temperature stress. Extent of up-regulation of expression following exposure to chilling differed for a number of genes including CCA1, NAC2, and SKIP16. Microarray analysis identified 207 genes that were differentially expressed between two focal populations, Leitrim (Ireland) and Helin (Norway), irrespective of temperature treatment (P <0.05, fold change >2). Approximately 6% of the A. l. petraea transcriptome was responsive to acclimation whilst expression of 75 genes, many annotated as unknown function, changed following exposure to freezing. The identified phenotypic variation is indicative of local adaptation, and populations from throughout the species’ range may thus harbour unique genetic variation. Further ... |
format |
Thesis |
author |
Thompson, Chloe Elizabeth |
spellingShingle |
Thompson, Chloe Elizabeth The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli |
author_facet |
Thompson, Chloe Elizabeth |
author_sort |
Thompson, Chloe Elizabeth |
title |
The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli |
title_short |
The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli |
title_full |
The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli |
title_fullStr |
The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli |
title_full_unstemmed |
The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli |
title_sort |
responses of arabidopsis lyrata ssp. petraea to environmental stimuli |
publisher |
University of Leeds |
publishDate |
2010 |
url |
https://etheses.whiterose.ac.uk/1448/ https://etheses.whiterose.ac.uk/1448/1/Chloe_Elizabeth_Thompson_PhD_2010.pdf https://etheses.whiterose.ac.uk/1448/2/Appendix_1.pdf https://etheses.whiterose.ac.uk/1448/3/Appendix_2a.pdf https://etheses.whiterose.ac.uk/1448/4/Appendix_2b.pdf https://etheses.whiterose.ac.uk/1448/5/Appendix_2c.pdf https://etheses.whiterose.ac.uk/1448/6/Appendix_2d.pdf https://etheses.whiterose.ac.uk/1448/7/Appendix_2e.pdf |
long_lat |
ENVELOPE(19.017,19.017,74.467,74.467) |
geographic |
Helin Norway |
geographic_facet |
Helin Norway |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
https://etheses.whiterose.ac.uk/1448/1/Chloe_Elizabeth_Thompson_PhD_2010.pdf https://etheses.whiterose.ac.uk/1448/2/Appendix_1.pdf https://etheses.whiterose.ac.uk/1448/3/Appendix_2a.pdf https://etheses.whiterose.ac.uk/1448/4/Appendix_2b.pdf https://etheses.whiterose.ac.uk/1448/5/Appendix_2c.pdf https://etheses.whiterose.ac.uk/1448/6/Appendix_2d.pdf https://etheses.whiterose.ac.uk/1448/7/Appendix_2e.pdf Thompson, Chloe Elizabeth (2010) The responses of Arabidopsis lyrata ssp. petraea to environmental stimuli. PhD thesis, University of Leeds. |
op_rights |
cc_by_nc_sa |
op_rightsnorm |
CC-BY-NC-SA |
_version_ |
1766038885251416064 |