Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods
The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2–3 million years. A total of 265 Bacteria 16S rRNA gene sequences were a...
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ftwestwashington:oai:cedar.wwu.edu:biology_facpubs-1029 2023-05-15T13:57:40+02:00 Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods Moyer, Craig L. 2006-01-01T08:00:00Z application/pdf https://cedar.wwu.edu/biology_facpubs/30 https://cedar.wwu.edu/cgi/viewcontent.cgi?article=1029&context=biology_facpubs English eng Western CEDAR https://cedar.wwu.edu/biology_facpubs/30 https://cedar.wwu.edu/cgi/viewcontent.cgi?article=1029&context=biology_facpubs Biology Faculty and Staff Publications psychrotroph arctic arthrobacter psychrobacter exiguobacterium 16S rRNA phylogeny Biology text 2006 ftwestwashington 2022-09-14T05:56:10Z The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2–3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at –2.5°C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System. Text Antarc* Antarctica Arctic permafrost Western Washington University: CEDAR (Contributing to Education through Digital Access to Research) Arctic Kolyma ENVELOPE(161.000,161.000,69.500,69.500) |
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Open Polar |
collection |
Western Washington University: CEDAR (Contributing to Education through Digital Access to Research) |
op_collection_id |
ftwestwashington |
language |
English |
topic |
psychrotroph arctic arthrobacter psychrobacter exiguobacterium 16S rRNA phylogeny Biology |
spellingShingle |
psychrotroph arctic arthrobacter psychrobacter exiguobacterium 16S rRNA phylogeny Biology Moyer, Craig L. Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods |
topic_facet |
psychrotroph arctic arthrobacter psychrobacter exiguobacterium 16S rRNA phylogeny Biology |
description |
The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2–3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at –2.5°C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System. |
format |
Text |
author |
Moyer, Craig L. |
author_facet |
Moyer, Craig L. |
author_sort |
Moyer, Craig L. |
title |
Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods |
title_short |
Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods |
title_full |
Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods |
title_fullStr |
Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods |
title_full_unstemmed |
Bacterial Community in Ancient Siberian Permafrost as Characterized by Culture and Culture-Independent Methods |
title_sort |
bacterial community in ancient siberian permafrost as characterized by culture and culture-independent methods |
publisher |
Western CEDAR |
publishDate |
2006 |
url |
https://cedar.wwu.edu/biology_facpubs/30 https://cedar.wwu.edu/cgi/viewcontent.cgi?article=1029&context=biology_facpubs |
long_lat |
ENVELOPE(161.000,161.000,69.500,69.500) |
geographic |
Arctic Kolyma |
geographic_facet |
Arctic Kolyma |
genre |
Antarc* Antarctica Arctic permafrost |
genre_facet |
Antarc* Antarctica Arctic permafrost |
op_source |
Biology Faculty and Staff Publications |
op_relation |
https://cedar.wwu.edu/biology_facpubs/30 https://cedar.wwu.edu/cgi/viewcontent.cgi?article=1029&context=biology_facpubs |
_version_ |
1766265438295031808 |