Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum)
PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty-eight primer pairs were desig...
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ftwestvirginiaun:oai:researchrepository.wvu.edu:faculty_publications-2381 2023-11-12T04:23:30+01:00 Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) Sheik, Mathew L. Labounty, Kitty L. Mitchell, Erika Gillespie, Emily L. 2019-01-01T08:00:00Z application/pdf https://researchrepository.wvu.edu/faculty_publications/1352 https://researchrepository.wvu.edu/context/faculty_publications/article/2381/viewcontent/Fourteen_polymorphic_microsatellite_markers_for_the.pdf unknown The Research Repository @ WVU https://researchrepository.wvu.edu/faculty_publications/1352 https://researchrepository.wvu.edu/context/faculty_publications/article/2381/viewcontent/Fourteen_polymorphic_microsatellite_markers_for_the.pdf Faculty & Staff Scholarship Ericaceae Labrador tea Rhodododendron groenlandicum Rhododendron subsect. Ledum species boundaries Biology text 2019 ftwestvirginiaun 2023-10-29T18:00:56Z PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty-eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross-amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well-supported clade. CONCLUSIONS: These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum Text Northwest Territories Alaska The Research Repository @ WVU (West Virginia University) Canada Northwest Territories |
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The Research Repository @ WVU (West Virginia University) |
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ftwestvirginiaun |
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Ericaceae Labrador tea Rhodododendron groenlandicum Rhododendron subsect. Ledum species boundaries Biology |
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Ericaceae Labrador tea Rhodododendron groenlandicum Rhododendron subsect. Ledum species boundaries Biology Sheik, Mathew L. Labounty, Kitty L. Mitchell, Erika Gillespie, Emily L. Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) |
topic_facet |
Ericaceae Labrador tea Rhodododendron groenlandicum Rhododendron subsect. Ledum species boundaries Biology |
description |
PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty-eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross-amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well-supported clade. CONCLUSIONS: These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum |
format |
Text |
author |
Sheik, Mathew L. Labounty, Kitty L. Mitchell, Erika Gillespie, Emily L. |
author_facet |
Sheik, Mathew L. Labounty, Kitty L. Mitchell, Erika Gillespie, Emily L. |
author_sort |
Sheik, Mathew L. |
title |
Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) |
title_short |
Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) |
title_full |
Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) |
title_fullStr |
Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) |
title_full_unstemmed |
Fourteen Polymorphic Microsatellite Markers for the Widespread Labrador Tea (Rhododendron groenlandicum) |
title_sort |
fourteen polymorphic microsatellite markers for the widespread labrador tea (rhododendron groenlandicum) |
publisher |
The Research Repository @ WVU |
publishDate |
2019 |
url |
https://researchrepository.wvu.edu/faculty_publications/1352 https://researchrepository.wvu.edu/context/faculty_publications/article/2381/viewcontent/Fourteen_polymorphic_microsatellite_markers_for_the.pdf |
geographic |
Canada Northwest Territories |
geographic_facet |
Canada Northwest Territories |
genre |
Northwest Territories Alaska |
genre_facet |
Northwest Territories Alaska |
op_source |
Faculty & Staff Scholarship |
op_relation |
https://researchrepository.wvu.edu/faculty_publications/1352 https://researchrepository.wvu.edu/context/faculty_publications/article/2381/viewcontent/Fourteen_polymorphic_microsatellite_markers_for_the.pdf |
_version_ |
1782338248879636480 |