Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny
Pentraxins are fluid phase pattern recognition molecules that form an important part of the innate immune defence and are conserved between fish and human. In Atlantic cod (Gadus morhua L.), two pentraxin-like proteins have been described, CRP-I and CRP-II. Here we show for the first time that these...
Published in: | Developmental & Comparative Immunology |
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Online Access: | https://westminsterresearch.westminster.ac.uk/item/q52w7/pentraxins-crp-i-and-crp-ii-are-post-translationally-deiminated-and-differ-in-tissue-specificity-in-cod-gadus-morhua-l-ontogeny https://westminsterresearch.westminster.ac.uk/download/ce77b48e6a558a7d266df1a1241cc477c9259a1c9294e78c53a70adc03aef5b1/2716711/1-s2.0-S0145305X18301307-main%283%29.pdf https://doi.org/10.1016/j.dci.2018.05.014 |
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ftwestminres:oai:westminsterresearch.westminster.ac.uk:q52w7 2023-05-15T15:27:42+02:00 Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny Magnadóttir, B. Hayes, P.M. Gisladottir, B. Bragason, B. Hristova, M. Nicholas, A.P. Gudmundsdottir, S. Lange, S. 2018 application/pdf https://westminsterresearch.westminster.ac.uk/item/q52w7/pentraxins-crp-i-and-crp-ii-are-post-translationally-deiminated-and-differ-in-tissue-specificity-in-cod-gadus-morhua-l-ontogeny https://westminsterresearch.westminster.ac.uk/download/ce77b48e6a558a7d266df1a1241cc477c9259a1c9294e78c53a70adc03aef5b1/2716711/1-s2.0-S0145305X18301307-main%283%29.pdf https://doi.org/10.1016/j.dci.2018.05.014 unknown Elsevier https://westminsterresearch.westminster.ac.uk/download/ce77b48e6a558a7d266df1a1241cc477c9259a1c9294e78c53a70adc03aef5b1/2716711/1-s2.0-S0145305X18301307-main%283%29.pdf https://doi.org/10.1016/j.dci.2018.05.014 Magnadóttir, B., Hayes, P.M., Gisladottir, B., Bragason, B., Hristova, M., Nicholas, A.P., Gudmundsdottir, S. and Lange, S. 2018. Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny. Developmental and Comparative Immunology. 87, pp. 1-11. https://doi.org/10.1016/j.dci.2018.05.014 CC BY 4.0 Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhua L.) Ontogeny journal-article PeerReviewed 2018 ftwestminres https://doi.org/10.1016/j.dci.2018.05.014 2023-03-26T20:37:39Z Pentraxins are fluid phase pattern recognition molecules that form an important part of the innate immune defence and are conserved between fish and human. In Atlantic cod (Gadus morhua L.), two pentraxin-like proteins have been described, CRP-I and CRP-II. Here we show for the first time that these two CRP forms are post-translationally deiminated (an irreversible conversion of arginine to citrulline) and differ with respect to tissue specific localisation in cod ontogeny from 3 to 84 days post hatching. While both forms are expressed in liver, albeit at temporally differing levels, CRP-I shows a strong association with nervous tissue while CRP-II is strongly associated to mucosal tissues of gut and skin. This indicates differing roles for the two pentraxin types in immune responses and tissue remodelling, also elucidating novel roles for CRP-I in the nervous system. The presence of deimination positive bands for cod CRPs varied somewhat between mucus and serum, possibly facilitating CRP protein moonlighting, allowing the same protein to exhibit a range of biological functions and thus meeting different functional requirements in different tissues. The presented findings may further current understanding of the diverse roles of pentraxins in teleost immune defences and tissue remodelling, as well as in various human pathologies, including autoimmune diseases, amyloidosis and cancer. Text atlantic cod Gadus morhua University of Westminster: WestminsterResearch Developmental & Comparative Immunology 87 1 11 |
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University of Westminster: WestminsterResearch |
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topic |
Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhua L.) Ontogeny |
spellingShingle |
Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhua L.) Ontogeny Magnadóttir, B. Hayes, P.M. Gisladottir, B. Bragason, B. Hristova, M. Nicholas, A.P. Gudmundsdottir, S. Lange, S. Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
topic_facet |
Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhua L.) Ontogeny |
description |
Pentraxins are fluid phase pattern recognition molecules that form an important part of the innate immune defence and are conserved between fish and human. In Atlantic cod (Gadus morhua L.), two pentraxin-like proteins have been described, CRP-I and CRP-II. Here we show for the first time that these two CRP forms are post-translationally deiminated (an irreversible conversion of arginine to citrulline) and differ with respect to tissue specific localisation in cod ontogeny from 3 to 84 days post hatching. While both forms are expressed in liver, albeit at temporally differing levels, CRP-I shows a strong association with nervous tissue while CRP-II is strongly associated to mucosal tissues of gut and skin. This indicates differing roles for the two pentraxin types in immune responses and tissue remodelling, also elucidating novel roles for CRP-I in the nervous system. The presence of deimination positive bands for cod CRPs varied somewhat between mucus and serum, possibly facilitating CRP protein moonlighting, allowing the same protein to exhibit a range of biological functions and thus meeting different functional requirements in different tissues. The presented findings may further current understanding of the diverse roles of pentraxins in teleost immune defences and tissue remodelling, as well as in various human pathologies, including autoimmune diseases, amyloidosis and cancer. |
format |
Text |
author |
Magnadóttir, B. Hayes, P.M. Gisladottir, B. Bragason, B. Hristova, M. Nicholas, A.P. Gudmundsdottir, S. Lange, S. |
author_facet |
Magnadóttir, B. Hayes, P.M. Gisladottir, B. Bragason, B. Hristova, M. Nicholas, A.P. Gudmundsdottir, S. Lange, S. |
author_sort |
Magnadóttir, B. |
title |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_short |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_full |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_fullStr |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_full_unstemmed |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_sort |
pentraxins crp-i and crp-ii are post-translationally deiminated and differ in tissue specificity in cod (gadus morhua l.) ontogeny |
publisher |
Elsevier |
publishDate |
2018 |
url |
https://westminsterresearch.westminster.ac.uk/item/q52w7/pentraxins-crp-i-and-crp-ii-are-post-translationally-deiminated-and-differ-in-tissue-specificity-in-cod-gadus-morhua-l-ontogeny https://westminsterresearch.westminster.ac.uk/download/ce77b48e6a558a7d266df1a1241cc477c9259a1c9294e78c53a70adc03aef5b1/2716711/1-s2.0-S0145305X18301307-main%283%29.pdf https://doi.org/10.1016/j.dci.2018.05.014 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://westminsterresearch.westminster.ac.uk/download/ce77b48e6a558a7d266df1a1241cc477c9259a1c9294e78c53a70adc03aef5b1/2716711/1-s2.0-S0145305X18301307-main%283%29.pdf https://doi.org/10.1016/j.dci.2018.05.014 Magnadóttir, B., Hayes, P.M., Gisladottir, B., Bragason, B., Hristova, M., Nicholas, A.P., Gudmundsdottir, S. and Lange, S. 2018. Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny. Developmental and Comparative Immunology. 87, pp. 1-11. https://doi.org/10.1016/j.dci.2018.05.014 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.1016/j.dci.2018.05.014 |
container_title |
Developmental & Comparative Immunology |
container_volume |
87 |
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1 |
op_container_end_page |
11 |
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