Molecular detection of Mycoplasma spp. in carnivores from Lithuania

• Main Authors: Sakalauskas, Povilas, Paulauskas, Algimantas, Radzijevskaja, Jana
• Format: Other/Unknown Material
• Language: English
• Published: 2018
• Subjects: Mycoplasma spp; Bacteria; Carnivores; Biologija / Biology (N010); Canis lupus; gray wolf
• Online Access: https://eltalpykla.vdu.lt/handle/1/36324

Mycoplasma is a genus of self-replicating bacterium. Most of them are responsible for variety of diseases in human, animal, insects and plants. There is a lack of information about Mycoplasma in wildlife animals. The aim of this study was to investigate the presence of Mycoplasma spp. in Carnivores from Lithuania. A total number of 45 animal samples were collected after car accident and by hunters: 31 red fox (Vulpes vulpes), 5 European otter (Lutra lurta), 5 beech marten (Martes foina), 2 common ferret (Mustela putorius), 1 gray wolf (Canis lupus) and 1 raccoon dog (Nyctereutes procyonoides). DNA was extracted from the spleen samples, and then used for PCR analysis. PCR was performed using the primers targeting a Mycoplasma sp. 600-bp region of the 16S rRNA gene. Amplification occurred in 5/5 (100 %) of L. lutra, 2/2 (100 %) of M. putorius and 1/5 (20 %) of M. foina. The Mycoplasma were not detected in V. vulpes, C. lupus, N. procyonoides. All positive samples sequences show 94 – 100 % similarity with Mycoplasma sp. Sequence from GenBank Biologijos katedra Gamtos mokslų fakultetas Vytauto Didžiojo universitetas