Genetic diversity of Bartonella strains in small rodents
Bartonella infections have been documented in a wide range of mammals and 15 Bartonella species have been detected in small rodents. Several rodent-associated Bartonella species have been related to human diseases. However, there is a lack of studies on the presence and diversity of Bartonella patho...
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ftvytmagnusuniv:oai:portalcris.vdu.lt:20.500.12259/59157 2023-05-15T17:12:37+02:00 Genetic diversity of Bartonella strains in small rodents Mardosaitė-Busaitienė, Dalytė Radzijevskaja, Jana Paulauskas, Algimantas Balčiauskas, Linas Bračikov, Maksim DE 2018 p. 253-253 text/xml https://rodents2018.org/wp/wp-content/uploads/2018/08/Book_of_Abstracts.pdf en eng Rodent biology and management and 16th rodens et spatium: 6th international conference, Potsdam, Germany, 3-7 September 2018: book of abstracts / editors Jens Jacob and Jana Eccard. Berlin : Arno Brynda GmbH, 2018 9783955470593 VDU02-000022757 https://rodents2018.org/wp/wp-content/uploads/2018/08/Book_of_Abstracts.pdf Bartonella strains Bartonella infections Rodents Biologija / Biology (N010) Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2) 2018 ftvytmagnusuniv 2020-04-20T23:40:19Z Bartonella infections have been documented in a wide range of mammals and 15 Bartonella species have been detected in small rodents. Several rodent-associated Bartonella species have been related to human diseases. However, there is a lack of studies on the presence and diversity of Bartonella pathogens in small rodents in Baltic region. The objectives of this study were to investigate the prevalence and genetic diversity of Bartonella strains in different species of small rodents from Lithuania. We collected spleens from seven small rodent species captured in different parts of Lithuania during 2013-2016. The presence of Bartonella was examined by real-time PCR targeting the ssrA gene. Species identification and molecular characterization of bacteria strains were based on sequence analysis of two housekeeping genes (rpoB, groEL) and the intergenic species region. Bartonella DNA was detected with different prevalence in Apodemus flavicollis, Micromys minutus, Myodes glareolus, Microtus oeconomus, Microtus agrestis and Microtus arvalis rodents. Sequence analysis of Bartonella isolates showed that the Bartonella strains circulating among the investigated rodents are heterogenic and belonged to Bartonella grahamii, Bartonella taylorii and Bartonella rochalimae genogroup. Phylogenetic analysis based on each of the targets demonstrated the presence of different Bartonella grahamii and Bartonella taylorii strains associated with different species of rodents. This is the first report on molecular characterization of Bartonella strains in multiple rodent species from Baltic region. Our findings provide evidence of wide distribution of human pathogenic Bartonella grahamii in Lithuania Aplinkotyros katedra Biologijos katedra Gamtos mokslų fakultetas Gamtos tyrimų centras Vytauto Didžiojo universitetas Other/Unknown Material Microtus arvalis Vytautas Magnus University e-Publication Repository (VMU ePub) |
institution |
Open Polar |
collection |
Vytautas Magnus University e-Publication Repository (VMU ePub) |
op_collection_id |
ftvytmagnusuniv |
language |
English |
topic |
Bartonella strains Bartonella infections Rodents Biologija / Biology (N010) |
spellingShingle |
Bartonella strains Bartonella infections Rodents Biologija / Biology (N010) Mardosaitė-Busaitienė, Dalytė Radzijevskaja, Jana Paulauskas, Algimantas Balčiauskas, Linas Bračikov, Maksim Genetic diversity of Bartonella strains in small rodents |
topic_facet |
Bartonella strains Bartonella infections Rodents Biologija / Biology (N010) |
description |
Bartonella infections have been documented in a wide range of mammals and 15 Bartonella species have been detected in small rodents. Several rodent-associated Bartonella species have been related to human diseases. However, there is a lack of studies on the presence and diversity of Bartonella pathogens in small rodents in Baltic region. The objectives of this study were to investigate the prevalence and genetic diversity of Bartonella strains in different species of small rodents from Lithuania. We collected spleens from seven small rodent species captured in different parts of Lithuania during 2013-2016. The presence of Bartonella was examined by real-time PCR targeting the ssrA gene. Species identification and molecular characterization of bacteria strains were based on sequence analysis of two housekeeping genes (rpoB, groEL) and the intergenic species region. Bartonella DNA was detected with different prevalence in Apodemus flavicollis, Micromys minutus, Myodes glareolus, Microtus oeconomus, Microtus agrestis and Microtus arvalis rodents. Sequence analysis of Bartonella isolates showed that the Bartonella strains circulating among the investigated rodents are heterogenic and belonged to Bartonella grahamii, Bartonella taylorii and Bartonella rochalimae genogroup. Phylogenetic analysis based on each of the targets demonstrated the presence of different Bartonella grahamii and Bartonella taylorii strains associated with different species of rodents. This is the first report on molecular characterization of Bartonella strains in multiple rodent species from Baltic region. Our findings provide evidence of wide distribution of human pathogenic Bartonella grahamii in Lithuania Aplinkotyros katedra Biologijos katedra Gamtos mokslų fakultetas Gamtos tyrimų centras Vytauto Didžiojo universitetas |
format |
Other/Unknown Material |
author |
Mardosaitė-Busaitienė, Dalytė Radzijevskaja, Jana Paulauskas, Algimantas Balčiauskas, Linas Bračikov, Maksim |
author_facet |
Mardosaitė-Busaitienė, Dalytė Radzijevskaja, Jana Paulauskas, Algimantas Balčiauskas, Linas Bračikov, Maksim |
author_sort |
Mardosaitė-Busaitienė, Dalytė |
title |
Genetic diversity of Bartonella strains in small rodents |
title_short |
Genetic diversity of Bartonella strains in small rodents |
title_full |
Genetic diversity of Bartonella strains in small rodents |
title_fullStr |
Genetic diversity of Bartonella strains in small rodents |
title_full_unstemmed |
Genetic diversity of Bartonella strains in small rodents |
title_sort |
genetic diversity of bartonella strains in small rodents |
publishDate |
2018 |
url |
https://rodents2018.org/wp/wp-content/uploads/2018/08/Book_of_Abstracts.pdf |
op_coverage |
DE |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
Rodent biology and management and 16th rodens et spatium: 6th international conference, Potsdam, Germany, 3-7 September 2018: book of abstracts / editors Jens Jacob and Jana Eccard. Berlin : Arno Brynda GmbH, 2018 9783955470593 VDU02-000022757 https://rodents2018.org/wp/wp-content/uploads/2018/08/Book_of_Abstracts.pdf |
_version_ |
1766069404324331520 |