Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers
DNA markers play a major role in genetic analysis of mammals. The aim of this study was to assess the genetic structure of roe deer in Lithuania using microsatellite and D-loop sequences analysis. Seven microsattelite derived from rein deer (Rangifer tarandus) with published primer sequences were us...
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ftvytmagnusuniv:oai:portalcris.vdu.lt:20.500.12259/48213 2023-05-15T18:04:21+02:00 Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas RU 2014 p. 226-230 text/xml http://www.rgazu.ru/db/vestohotoved/11_2_2014.pdf en eng Вестник охотоведения : научно-практический и теоретический журнал. Балашиха : Российский государственный аграрный заочный университет, 2014, т. 11, № 2 VINITI 1994411X VDU02-000018110 http://www.rgazu.ru/db/vestohotoved/11_2_2014.pdf mtDNA D-loop Microsatellite Roe deer Capreolus capreolus Genetic diversity Biologija / Biology (N010) Straipsnis kitose duomenų bazėse / Article in other databases (S4) 2014 ftvytmagnusuniv 2020-12-22T00:44:07Z DNA markers play a major role in genetic analysis of mammals. The aim of this study was to assess the genetic structure of roe deer in Lithuania using microsatellite and D-loop sequences analysis. Seven microsattelite derived from rein deer (Rangifer tarandus) with published primer sequences were used. Total number of alleles per locus varied from 2 to 9 and number of effective alleles varied from 2,0 to 7,5. Analysis of molecular variance (AMOVA) showed that genetic diversity of the populations yielded 79 % of the total genetic diversity and among populations 21 % of the total genetic diversity. Mitochondrial DNA was amplified using L-Pro and H-493 primers, which amplify a 436 bp fragment from the first hypervariable domain of the mtDNA control region. Six haplotypes and two haplogroups were identified in population of roe deer in Lithuania. A total 41 variable sites were detected in mtDNA variable region. H2, H3, H4, H5 and H6 haplotypes were specific in roe deer from Lithuania. Haplotype diversity Hd = 0.854 and nucleotide diversity Pi = 0.01981 were calculated. Results of DNA markers analysis revealed high level molecular genetic variation in roe deer population in Lithuania Biologijos katedra Vytauto Didžiojo universitetas Other/Unknown Material Rangifer tarandus Vytautas Magnus University e-Publication Repository (VMU ePub) |
institution |
Open Polar |
collection |
Vytautas Magnus University e-Publication Repository (VMU ePub) |
op_collection_id |
ftvytmagnusuniv |
language |
English |
topic |
mtDNA D-loop Microsatellite Roe deer Capreolus capreolus Genetic diversity Biologija / Biology (N010) |
spellingShingle |
mtDNA D-loop Microsatellite Roe deer Capreolus capreolus Genetic diversity Biologija / Biology (N010) Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
topic_facet |
mtDNA D-loop Microsatellite Roe deer Capreolus capreolus Genetic diversity Biologija / Biology (N010) |
description |
DNA markers play a major role in genetic analysis of mammals. The aim of this study was to assess the genetic structure of roe deer in Lithuania using microsatellite and D-loop sequences analysis. Seven microsattelite derived from rein deer (Rangifer tarandus) with published primer sequences were used. Total number of alleles per locus varied from 2 to 9 and number of effective alleles varied from 2,0 to 7,5. Analysis of molecular variance (AMOVA) showed that genetic diversity of the populations yielded 79 % of the total genetic diversity and among populations 21 % of the total genetic diversity. Mitochondrial DNA was amplified using L-Pro and H-493 primers, which amplify a 436 bp fragment from the first hypervariable domain of the mtDNA control region. Six haplotypes and two haplogroups were identified in population of roe deer in Lithuania. A total 41 variable sites were detected in mtDNA variable region. H2, H3, H4, H5 and H6 haplotypes were specific in roe deer from Lithuania. Haplotype diversity Hd = 0.854 and nucleotide diversity Pi = 0.01981 were calculated. Results of DNA markers analysis revealed high level molecular genetic variation in roe deer population in Lithuania Biologijos katedra Vytauto Didžiojo universitetas |
format |
Other/Unknown Material |
author |
Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas |
author_facet |
Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas |
author_sort |
Pūraitė, Irma |
title |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_short |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_full |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_fullStr |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_full_unstemmed |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_sort |
genetic analysis of roe deer (capreolus capreolus l.) using dna markers |
publishDate |
2014 |
url |
http://www.rgazu.ru/db/vestohotoved/11_2_2014.pdf |
op_coverage |
RU |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_relation |
Вестник охотоведения : научно-практический и теоретический журнал. Балашиха : Российский государственный аграрный заочный университет, 2014, т. 11, № 2 VINITI 1994411X VDU02-000018110 http://www.rgazu.ru/db/vestohotoved/11_2_2014.pdf |
_version_ |
1766175706196213760 |