The Light Responses of Proteorhodopsin-bearing, Antarctic Sea-ice Bacteria

• Main Author: Burr, David Joll
• Other Authors: Ryan, Ken, Maas, Els
• Format: Master Thesis
• Language: English
• Published: Victoria University of Wellington 2014
• Subjects: Proteorhodopsin; Antarctic; Bacteria; Antarc*; Sea ice
• Online Access: http://researcharchive.vuw.ac.nz/handle/10063/3675

Although homogenous in appearance, Antarctic sea ice forms a complex habitat that is characterised by steep vertical gradients of temperature, irradiance and salinity. Despite these harsh and variable environmental conditions, numerous microbial organisms prosper within Antarctic sea ice. In 2010, bacteria bearing the proteorhodopsin (PR) gene were found within Antarctic sea ice. PR is a photoactive membrane protein that functions as a light-driven proton pump. The hydrogen ion membrane gradient that PR establishes has the potential to drive ATP synthesis, thus allowing PR-bearing bacteria to obtain energy from solar radiation. Although this gene is present in up to 80% of marine bacteria, the active contribution of PR in vivo is debatable. Light induced growth or enhanced survival is generally observed only when PR-bearing bacteria are grown under sub-optimum conditions, such as limited nutrients or carbon, or variations in salinity. This has lead to the general hypothesis that PR has multiple functions, becoming most influential under conditions of stress. In this way, Antarctic sea-ice bacteria may utilise PR to promote survival and enhance energy inputs, when exposed to the harsh conditions of this environment. To explore this hypothesis, potential PR-bearing isolates were cultured from samples of Antarctic sea-ice bacteria. Using 16S rRNA gene sequencing as well as a comparison of phenotypic and environmental characteristics, the isolates were identified as; Psychrobacter nivimaris, Polaribacter dokdonensis, Paracoccus marcusii and Micrococcus sp. These species, along with Psychroflexus torquis (an Antarctic sea-ice bacterium known to possess PR) were examined for the presence of the PR gene. This gene was identified in P. torquis, Ps. nivimaris and Po. dokdonensis. To my knowledge, this is the first time PR has been found in Ps. nivimaris. To assess the influence of irradiance on these species, a series of culture based experiments were undertaken. In 2012, a preliminary field experiment was conducted in ...