Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells
Immune checkpoint blockade has resulted in durable responses in patients with metastatic melanoma, but only in a fraction of treated patients. For immune checkpoint inhibitors (ICI) to be effective, sufficient infiltration with tumor-reactive T cells is essential. Oncolytic viruses (OV) selectively...
Published in: | Clinical and Experimental Immunology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2020
|
Subjects: | |
Online Access: | https://research.vumc.nl/en/publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 https://doi.org/10.1111/cei.13442 http://www.scopus.com/inward/record.url?scp=85085098609&partnerID=8YFLogxK |
id |
ftvuamsterumc:oai:pure.atira.dk:publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 |
---|---|
record_format |
openpolar |
spelling |
ftvuamsterumc:oai:pure.atira.dk:publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 2024-09-30T14:40:56+00:00 Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells López González, M. van de Ven, R. de Haan, H. van Eck van der Sluijs, J. Dong, W. van Beusechem, V. W. de Gruijl, T. D. 2020-08-01 https://research.vumc.nl/en/publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 https://doi.org/10.1111/cei.13442 http://www.scopus.com/inward/record.url?scp=85085098609&partnerID=8YFLogxK eng eng https://research.vumc.nl/en/publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 info:eu-repo/semantics/openAccess López González , M , van de Ven , R , de Haan , H , van Eck van der Sluijs , J , Dong , W , van Beusechem , V W & de Gruijl , T D 2020 , ' Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells ' , Clinical and Experimental Immunology , vol. 201 , no. 2 , pp. 145-160 . https://doi.org/10.1111/cei.13442 article 2020 ftvuamsterumc https://doi.org/10.1111/cei.13442 2024-09-05T01:16:26Z Immune checkpoint blockade has resulted in durable responses in patients with metastatic melanoma, but only in a fraction of treated patients. For immune checkpoint inhibitors (ICI) to be effective, sufficient infiltration with tumor-reactive T cells is essential. Oncolytic viruses (OV) selectively replicate in and lyse tumor cells and so induce an immunogenic form of cell death, providing at once a source of tumor-associated (neo)antigens and of danger signals that together induce effective T cell immunity and tumor infiltration. Melanoma-associated suppression of dendritic cell (DC) differentiation effectively hampers OV- or immune checkpoint inhibitor (ICI)-induced anti-tumor immunity, due to a consequent inability to prime and attract anti-tumor effector T cells. Here, we set out to study the effect of ORCA-010, a clinical stage oncolytic adenovirus, on DC differentiation and functionality in the context of human melanoma. In melanoma and monocyte co-cultures, employing a panel of five melanoma cell lines with varying origins and oncogenic mutation status, we observed clear suppression of DC development with apparent skewing of monocyte differentiation to a more M2-macrophage-like state. We established the ability of ORCA-010 to productively infect and lyse the melanoma cells. Moreover, although ORCA-010 was unable to restore DC differentiation, it induced activation and an increased co-stimulatory capacity of monocyte-derived antigen-presenting cells. Their subsequent ability to prime effector T cells with a type I cytokine profile was significantly increased in an allogeneic mixed leukocyte reaction. Our findings suggest that ORCA-010 is a valuable immunotherapeutic agent for melanoma. Article in Journal/Newspaper Orca Research portal Amsterdam UMC (Vrije Universiteit Amsterdam, Universitair Medische Centra) Clinical and Experimental Immunology 201 2 145 160 |
institution |
Open Polar |
collection |
Research portal Amsterdam UMC (Vrije Universiteit Amsterdam, Universitair Medische Centra) |
op_collection_id |
ftvuamsterumc |
language |
English |
description |
Immune checkpoint blockade has resulted in durable responses in patients with metastatic melanoma, but only in a fraction of treated patients. For immune checkpoint inhibitors (ICI) to be effective, sufficient infiltration with tumor-reactive T cells is essential. Oncolytic viruses (OV) selectively replicate in and lyse tumor cells and so induce an immunogenic form of cell death, providing at once a source of tumor-associated (neo)antigens and of danger signals that together induce effective T cell immunity and tumor infiltration. Melanoma-associated suppression of dendritic cell (DC) differentiation effectively hampers OV- or immune checkpoint inhibitor (ICI)-induced anti-tumor immunity, due to a consequent inability to prime and attract anti-tumor effector T cells. Here, we set out to study the effect of ORCA-010, a clinical stage oncolytic adenovirus, on DC differentiation and functionality in the context of human melanoma. In melanoma and monocyte co-cultures, employing a panel of five melanoma cell lines with varying origins and oncogenic mutation status, we observed clear suppression of DC development with apparent skewing of monocyte differentiation to a more M2-macrophage-like state. We established the ability of ORCA-010 to productively infect and lyse the melanoma cells. Moreover, although ORCA-010 was unable to restore DC differentiation, it induced activation and an increased co-stimulatory capacity of monocyte-derived antigen-presenting cells. Their subsequent ability to prime effector T cells with a type I cytokine profile was significantly increased in an allogeneic mixed leukocyte reaction. Our findings suggest that ORCA-010 is a valuable immunotherapeutic agent for melanoma. |
format |
Article in Journal/Newspaper |
author |
López González, M. van de Ven, R. de Haan, H. van Eck van der Sluijs, J. Dong, W. van Beusechem, V. W. de Gruijl, T. D. |
spellingShingle |
López González, M. van de Ven, R. de Haan, H. van Eck van der Sluijs, J. Dong, W. van Beusechem, V. W. de Gruijl, T. D. Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells |
author_facet |
López González, M. van de Ven, R. de Haan, H. van Eck van der Sluijs, J. Dong, W. van Beusechem, V. W. de Gruijl, T. D. |
author_sort |
López González, M. |
title |
Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells |
title_short |
Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells |
title_full |
Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells |
title_fullStr |
Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells |
title_full_unstemmed |
Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells |
title_sort |
oncolytic adenovirus orca-010 increases the type 1 t cell stimulatory capacity of melanoma-conditioned dendritic cells |
publishDate |
2020 |
url |
https://research.vumc.nl/en/publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 https://doi.org/10.1111/cei.13442 http://www.scopus.com/inward/record.url?scp=85085098609&partnerID=8YFLogxK |
genre |
Orca |
genre_facet |
Orca |
op_source |
López González , M , van de Ven , R , de Haan , H , van Eck van der Sluijs , J , Dong , W , van Beusechem , V W & de Gruijl , T D 2020 , ' Oncolytic adenovirus ORCA-010 increases the type 1 T cell stimulatory capacity of melanoma-conditioned dendritic cells ' , Clinical and Experimental Immunology , vol. 201 , no. 2 , pp. 145-160 . https://doi.org/10.1111/cei.13442 |
op_relation |
https://research.vumc.nl/en/publications/34e9cf7f-8d70-4ffd-b92c-7119fec00ce0 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/cei.13442 |
container_title |
Clinical and Experimental Immunology |
container_volume |
201 |
container_issue |
2 |
container_start_page |
145 |
op_container_end_page |
160 |
_version_ |
1811643387696119808 |