Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids

• Published in: International Dairy Journal
• Main Authors: Nyyssölä, Antti, Miettinen, Hanna, Kontkanen, Hanna, Lille, Martina, Partanen, Riitta, Rokka, Susanna, Järvenpää, Eila, Lantto, Raija, Kruus, Kristiina
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2015
• Subjects: Rugosa; Antarc*; Antarctica
• Online Access: https://cris.vtt.fi/en/publications/de7972a0-be3e-416e-b157-5bd5d0393cc7; https://doi.org/10.1016/j.idairyj.2014.09.003

Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28%, w/w) and increased the proportion of unsaturated ones (content of 52%, w/w) at the degree of hydrolysis of 48% (mol/mol) in relation to the unhydrolysed substrate (47%, w/w, C12:0-C14:0 and 32%, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.