First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine
Metallurgical processes demand large quantities of water. However, in many locations, water is becoming scarce and process water recycling is needed. Closing water loops can be challenging due to build-up of flotation chemicals, metal ions and microorganisms in the recycled water affecting the flota...
Published in: | Research in Microbiology |
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2020
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ftvttcrispub:oai:cris.vtt.fi:publications/641bc884-9851-4599-a27e-d7b865ed7fd2 2024-09-30T14:40:08+00:00 First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine Bomberg, Malin Miettinen, Hanna Musuku, Benjamin Kinnunen, Päivi 2020-10-01 https://cris.vtt.fi/en/publications/641bc884-9851-4599-a27e-d7b865ed7fd2 https://doi.org/10.1016/j.resmic.2020.07.001 http://www.scopus.com/inward/record.url?scp=85091100614&partnerID=8YFLogxK eng eng https://cris.vtt.fi/en/publications/641bc884-9851-4599-a27e-d7b865ed7fd2 info:eu-repo/semantics/openAccess Bomberg , M , Miettinen , H , Musuku , B & Kinnunen , P 2020 , ' First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine ' , Research in Microbiology , vol. 171 , no. 7 , pp. 230-242 . https://doi.org/10.1016/j.resmic.2020.07.001 bacteria fungi nickel mine copper mine floattion chemicals microbial metabolism /dk/atira/pure/sustainabledevelopmentgoals/clean_water_and_sanitation name=SDG 6 - Clean Water and Sanitation article 2020 ftvttcrispub https://doi.org/10.1016/j.resmic.2020.07.001 2024-09-11T23:36:42Z Metallurgical processes demand large quantities of water. However, in many locations, water is becoming scarce and process water recycling is needed. Closing water loops can be challenging due to build-up of flotation chemicals, metal ions and microorganisms in the recycled water affecting the flotation performance. Here, we have characterized the microbial communities over a 2-month period in different locations of the multi-metal Kevitsa mine in Northern Finland, by microbiome sequencing, enumeration of bacteria, archaea and fungi by qPCR, and cultivation. The microbial communities showed high diversity, but were dominated by Alpha- and Gammaproteobacteria. In addition, various fungal taxa were detected, whereas the archaeal taxa were only sparsely detected from the sequence data. The number of bacterial 16S rRNA gene copies in Process water and Ni thickener overflow varied between 0.5–3.3 × 10 5 mL −1 , whereas the Flotation tailings showed two orders of magnitude lower amounts. Fungi were present at 3.0 × 10 2 –8.1 × 10 4 5.8S rRNA gene copies mL −1 in all samples, while the number of archaea fluctuated between 8.8 × 10 1 –3.2 × 10 5 16S rRNA gene copies mL −1 . The number of all microbial groups were generally lower in September than in August. When tested on 8 different cultivation media, the microorganisms generally responded positively to organic carbon, and were also shown to oxidize thiosulfate, which may indicate that build-up of organic flotation chemicals and sulfur species from the ore may cause the microbial numbers to increase. This study is part of the H2020 ITERAMS project (Grant agreement# 730480), which strives to improve the recycling of water and minimize the environmental impact of mines. Article in Journal/Newspaper Northern Finland VTT's Research Information Portal Copper Mine ENVELOPE(-59.667,-59.667,-62.383,-62.383) Research in Microbiology 171 7 230 242 |
institution |
Open Polar |
collection |
VTT's Research Information Portal |
op_collection_id |
ftvttcrispub |
language |
English |
topic |
bacteria fungi nickel mine copper mine floattion chemicals microbial metabolism /dk/atira/pure/sustainabledevelopmentgoals/clean_water_and_sanitation name=SDG 6 - Clean Water and Sanitation |
spellingShingle |
bacteria fungi nickel mine copper mine floattion chemicals microbial metabolism /dk/atira/pure/sustainabledevelopmentgoals/clean_water_and_sanitation name=SDG 6 - Clean Water and Sanitation Bomberg, Malin Miettinen, Hanna Musuku, Benjamin Kinnunen, Päivi First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine |
topic_facet |
bacteria fungi nickel mine copper mine floattion chemicals microbial metabolism /dk/atira/pure/sustainabledevelopmentgoals/clean_water_and_sanitation name=SDG 6 - Clean Water and Sanitation |
description |
Metallurgical processes demand large quantities of water. However, in many locations, water is becoming scarce and process water recycling is needed. Closing water loops can be challenging due to build-up of flotation chemicals, metal ions and microorganisms in the recycled water affecting the flotation performance. Here, we have characterized the microbial communities over a 2-month period in different locations of the multi-metal Kevitsa mine in Northern Finland, by microbiome sequencing, enumeration of bacteria, archaea and fungi by qPCR, and cultivation. The microbial communities showed high diversity, but were dominated by Alpha- and Gammaproteobacteria. In addition, various fungal taxa were detected, whereas the archaeal taxa were only sparsely detected from the sequence data. The number of bacterial 16S rRNA gene copies in Process water and Ni thickener overflow varied between 0.5–3.3 × 10 5 mL −1 , whereas the Flotation tailings showed two orders of magnitude lower amounts. Fungi were present at 3.0 × 10 2 –8.1 × 10 4 5.8S rRNA gene copies mL −1 in all samples, while the number of archaea fluctuated between 8.8 × 10 1 –3.2 × 10 5 16S rRNA gene copies mL −1 . The number of all microbial groups were generally lower in September than in August. When tested on 8 different cultivation media, the microorganisms generally responded positively to organic carbon, and were also shown to oxidize thiosulfate, which may indicate that build-up of organic flotation chemicals and sulfur species from the ore may cause the microbial numbers to increase. This study is part of the H2020 ITERAMS project (Grant agreement# 730480), which strives to improve the recycling of water and minimize the environmental impact of mines. |
format |
Article in Journal/Newspaper |
author |
Bomberg, Malin Miettinen, Hanna Musuku, Benjamin Kinnunen, Päivi |
author_facet |
Bomberg, Malin Miettinen, Hanna Musuku, Benjamin Kinnunen, Päivi |
author_sort |
Bomberg, Malin |
title |
First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine |
title_short |
First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine |
title_full |
First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine |
title_fullStr |
First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine |
title_full_unstemmed |
First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine |
title_sort |
first insights to the microbial communities in the plant process water of the multi-metal kevitsa mine |
publishDate |
2020 |
url |
https://cris.vtt.fi/en/publications/641bc884-9851-4599-a27e-d7b865ed7fd2 https://doi.org/10.1016/j.resmic.2020.07.001 http://www.scopus.com/inward/record.url?scp=85091100614&partnerID=8YFLogxK |
long_lat |
ENVELOPE(-59.667,-59.667,-62.383,-62.383) |
geographic |
Copper Mine |
geographic_facet |
Copper Mine |
genre |
Northern Finland |
genre_facet |
Northern Finland |
op_source |
Bomberg , M , Miettinen , H , Musuku , B & Kinnunen , P 2020 , ' First insights to the microbial communities in the plant process water of the multi-metal Kevitsa mine ' , Research in Microbiology , vol. 171 , no. 7 , pp. 230-242 . https://doi.org/10.1016/j.resmic.2020.07.001 |
op_relation |
https://cris.vtt.fi/en/publications/641bc884-9851-4599-a27e-d7b865ed7fd2 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.resmic.2020.07.001 |
container_title |
Research in Microbiology |
container_volume |
171 |
container_issue |
7 |
container_start_page |
230 |
op_container_end_page |
242 |
_version_ |
1811642666975232000 |