Enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot ( Scophthalmus maximus ) larvae: a new member of the family Vibrionaceae

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Thompson, F.L., Hoste, B., Thompson, C.C., Goris, J., Gomez-Gil, B., Huys, L., de Vos, P., Swings, J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2002
• Subjects: Bacteria; Cultured organisms; DNA; Gutting; Hybridization; Larvae; Phylogenetics; Scophthalmus maximus (Linneaus; 1758); Vibrio; Norway; Scophthalmus maximus; Turbot
• Online Access: https://www.vliz.be/imisdocs/publications/335918.pdf

Twenty-two isolates originating from the gut of healthy cultured turbot larvae in Norway were investigated using a polyphasic approach. Amplified fragment length polymorphism fingerprinting analysis showed that the isolates have typical patterns and form two main groups. Phylogenetic analysis revealed that the isolates belong to the gamma- Proteobacteria , with Vibrio hollisae as their closest neighbour. DNA-DNA hybridization, chemotaxonomic and phenotypic analyses further proved that these isolates represent a tight novel taxon that differs from currently described species in the family Vibrionaceae . It is proposed that these novel isolates be accommodated in a new genus, Enterovibrio gen. nov., with Enterovibrio norvegicus sp. nov. as the type species. Isolates were motile by a polar flagellum, positive for oxidase, catalase, arginine dihydrolase and beta-galactosidase, but negative for the Voges--Proskauer reaction. They produced indole, did not reduce nitrate and were resistant to the vibriostatic agent O/129. The DNA G+C content of E. norvegicus was 47.1 to 47.9 %. The type strain is E. norvegicus LMG 19839(T) (=CAIM 430(T)).