Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics
Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill fun...
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ftvliz:oai:oma.vliz.be:353617 2023-05-15T15:31:23+02:00 Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics West, A.C. Mizoro, Y. Wood, S.H. Ince, L.M. Iversen, M. Jørgensen, E.H. Nome, T. Sandve, S.R. Martin, S.A.M. Loudon, A.S.I. Hazlerigg, D.G. 2021 application/pdf https://www.vliz.be/imisdocs/publications/379067.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000651406200001 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.3389/fimmu.2021.669889 https://www.vliz.be/imisdocs/publications/379067.pdf info:eu-repo/semantics/openAccess %3Ci%3EFrontiers+in+Immunology+12%3C%2Fi%3E%3A+669889.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.3389%2Ffimmu.2021.669889%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.3389%2Ffimmu.2021.669889%3C%2Fa%3E Salmo salar info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftvliz https://doi.org/10.3389/fimmu.2021.669889 2022-07-13T22:23:26Z Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill function to cope with the profound osmotic and immunological challenges of seawater (SW) migration. While prior work has highlighted the role of specialized “mitochondrion-rich” cells (MRCs) and accessory cells (ACs) in delivering this phenotype, recent RNA profiling experiments suggest that remodeling is far more extensive than previously appreciated. Here, we use single-nuclei RNAseq to characterize the extent of cytological changes in the gill of Atlantic salmon during smoltification and SW transfer. We identify 20 distinct cell clusters, including known, but also novel gill cell types. These data allow us to isolate cluster-specific, smoltification-associated changes in gene expression and to describe how the cellular make-up of the gill changes through smoltification. As expected, we noted an increase in the proportion of seawater mitochondrion-rich cells, however, we also identify previously unknown reduction of several immune-related cell types. Overall, our results provide fresh detail of the cellular complexity in the gill and suggest that smoltification triggers unexpected immune reprogramming. Article in Journal/Newspaper Atlantic salmon Salmo salar Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) Frontiers in Immunology 12 |
institution |
Open Polar |
collection |
Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
op_collection_id |
ftvliz |
language |
English |
topic |
Salmo salar |
spellingShingle |
Salmo salar West, A.C. Mizoro, Y. Wood, S.H. Ince, L.M. Iversen, M. Jørgensen, E.H. Nome, T. Sandve, S.R. Martin, S.A.M. Loudon, A.S.I. Hazlerigg, D.G. Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics |
topic_facet |
Salmo salar |
description |
Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill function to cope with the profound osmotic and immunological challenges of seawater (SW) migration. While prior work has highlighted the role of specialized “mitochondrion-rich” cells (MRCs) and accessory cells (ACs) in delivering this phenotype, recent RNA profiling experiments suggest that remodeling is far more extensive than previously appreciated. Here, we use single-nuclei RNAseq to characterize the extent of cytological changes in the gill of Atlantic salmon during smoltification and SW transfer. We identify 20 distinct cell clusters, including known, but also novel gill cell types. These data allow us to isolate cluster-specific, smoltification-associated changes in gene expression and to describe how the cellular make-up of the gill changes through smoltification. As expected, we noted an increase in the proportion of seawater mitochondrion-rich cells, however, we also identify previously unknown reduction of several immune-related cell types. Overall, our results provide fresh detail of the cellular complexity in the gill and suggest that smoltification triggers unexpected immune reprogramming. |
format |
Article in Journal/Newspaper |
author |
West, A.C. Mizoro, Y. Wood, S.H. Ince, L.M. Iversen, M. Jørgensen, E.H. Nome, T. Sandve, S.R. Martin, S.A.M. Loudon, A.S.I. Hazlerigg, D.G. |
author_facet |
West, A.C. Mizoro, Y. Wood, S.H. Ince, L.M. Iversen, M. Jørgensen, E.H. Nome, T. Sandve, S.R. Martin, S.A.M. Loudon, A.S.I. Hazlerigg, D.G. |
author_sort |
West, A.C. |
title |
Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics |
title_short |
Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics |
title_full |
Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics |
title_fullStr |
Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics |
title_full_unstemmed |
Immunologic profiling of the Atlantic salmon gill by single nuclei transcriptomics |
title_sort |
immunologic profiling of the atlantic salmon gill by single nuclei transcriptomics |
publishDate |
2021 |
url |
https://www.vliz.be/imisdocs/publications/379067.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
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op_relation |
info:eu-repo/semantics/altIdentifier/wos/000651406200001 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.3389/fimmu.2021.669889 https://www.vliz.be/imisdocs/publications/379067.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fimmu.2021.669889 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
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1766361883790540800 |