Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria
Cyanobacteria are generally thought to be responsible for primary production and nitrogen fixation in the microbial communities that dominate Antarctic ecosystems. Recent studies of bacterial communities in terrestrial Antarctica, however, have shown that Cyanobacteria are sometimes only scarcely pr...
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ftvliz:oai:oma.vliz.be:288808 2023-05-15T13:54:11+02:00 Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria Tahon, G. Tytgat, B. Stragier, P. Willems, A. 2016 http://www.vliz.be/nl/open-marien-archief?module=ref&refid=288808 en eng info:eu-repo/semantics/altIdentifier/wos/000367097500014 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1007/s00248-015-0704-6 http://www.vliz.be/nl/open-marien-archief?module=ref&refid=288808 info:eu-repo/semantics/restrictedAccess %3Ci%3EEnviron.+Microbiol.+71%281%29%3C%2Fi%3E%3A+131-149.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1007%2Fs00248-015-0704-6%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1007%2Fs00248-015-0704-6%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftvliz https://doi.org/10.1007/s00248-015-0704-6 2022-05-01T10:54:11Z Cyanobacteria are generally thought to be responsible for primary production and nitrogen fixation in the microbial communities that dominate Antarctic ecosystems. Recent studies of bacterial communities in terrestrial Antarctica, however, have shown that Cyanobacteria are sometimes only scarcely present, suggesting that other bacteria presumably take over their role as primary producers and diazotrophs. The diversity of key genes in these processes was studied in surface samples from the Sør Rondane Mountains, Dronning Maud Land, using clone libraries of the large subunit of ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) genes (cbbL, cbbM) and dinitrogenase-reductase (nifH) genes. We recovered a large diversity of non-cyanobacterial cbbL type IC in addition to cyanobacterial type IB, suggesting that non-cyanobacterial autotrophs may contribute to primary production. The nifH diversity recovered was predominantly related to Cyanobacteria, particularly members of the Nostocales. We also investigated the occurrence of proteorhodopsin and anoxygenic phototrophy as mechanisms for non-Cyanobacteria to exploit solar energy. While proteorhodopsin genes were not detected, a large diversity of genes coding for the light and medium subunits of the type 2 phototrophic reaction center (pufLM) was observed, suggesting for the first time, that the aerobic photoheterotrophic lifestyle may be important in oligotrophic high-altitude ice-free terrestrial Antarctic habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctica Dronning Maud Land Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) Antarctic Dronning Maud Land Sør Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) Sør-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) Microbial Ecology 71 1 131 149 |
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Open Polar |
collection |
Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
op_collection_id |
ftvliz |
language |
English |
description |
Cyanobacteria are generally thought to be responsible for primary production and nitrogen fixation in the microbial communities that dominate Antarctic ecosystems. Recent studies of bacterial communities in terrestrial Antarctica, however, have shown that Cyanobacteria are sometimes only scarcely present, suggesting that other bacteria presumably take over their role as primary producers and diazotrophs. The diversity of key genes in these processes was studied in surface samples from the Sør Rondane Mountains, Dronning Maud Land, using clone libraries of the large subunit of ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) genes (cbbL, cbbM) and dinitrogenase-reductase (nifH) genes. We recovered a large diversity of non-cyanobacterial cbbL type IC in addition to cyanobacterial type IB, suggesting that non-cyanobacterial autotrophs may contribute to primary production. The nifH diversity recovered was predominantly related to Cyanobacteria, particularly members of the Nostocales. We also investigated the occurrence of proteorhodopsin and anoxygenic phototrophy as mechanisms for non-Cyanobacteria to exploit solar energy. While proteorhodopsin genes were not detected, a large diversity of genes coding for the light and medium subunits of the type 2 phototrophic reaction center (pufLM) was observed, suggesting for the first time, that the aerobic photoheterotrophic lifestyle may be important in oligotrophic high-altitude ice-free terrestrial Antarctic habitats. |
format |
Article in Journal/Newspaper |
author |
Tahon, G. Tytgat, B. Stragier, P. Willems, A. |
spellingShingle |
Tahon, G. Tytgat, B. Stragier, P. Willems, A. Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
author_facet |
Tahon, G. Tytgat, B. Stragier, P. Willems, A. |
author_sort |
Tahon, G. |
title |
Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
title_short |
Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
title_full |
Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
title_fullStr |
Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
title_full_unstemmed |
Analysis of cbbL , nifH , and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
title_sort |
analysis of cbbl , nifh , and puflm in soils from the sør rondane mountains, antarctica, reveals a large diversity of autotrophic and phototrophic bacteria |
publishDate |
2016 |
url |
http://www.vliz.be/nl/open-marien-archief?module=ref&refid=288808 |
long_lat |
ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) |
geographic |
Antarctic Dronning Maud Land Sør Rondane Mountains Sør-Rondane |
geographic_facet |
Antarctic Dronning Maud Land Sør Rondane Mountains Sør-Rondane |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land |
op_source |
%3Ci%3EEnviron.+Microbiol.+71%281%29%3C%2Fi%3E%3A+131-149.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1007%2Fs00248-015-0704-6%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1007%2Fs00248-015-0704-6%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000367097500014 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1007/s00248-015-0704-6 http://www.vliz.be/nl/open-marien-archief?module=ref&refid=288808 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00248-015-0704-6 |
container_title |
Microbial Ecology |
container_volume |
71 |
container_issue |
1 |
container_start_page |
131 |
op_container_end_page |
149 |
_version_ |
1766259854342619136 |