Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica
Microbial life in exposed terrestrial surface layers in continental Antarctica is faced with extreme environmental conditions, including scarcity of organic matter. Bacteria in these exposed settings can therefore be expected to use alternative energy sources such as solar energy, abundant during th...
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ftvliz:oai:oma.vliz.be:288804 2023-05-15T13:54:11+02:00 Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica Tahon, G. Tytgat, B. Willems, A. 2016 application/pdf https://www.vliz.be/imisdocs/publications/305690.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000389952900001 info:eu-repo/semantics/altIdentifier/doi/.org/10.3389/fmicb.2016.02026 https://www.vliz.be/imisdocs/publications/305690.pdf info:eu-repo/semantics/openAccess %3Ci%3EFront.+Microbiol.+7%282026%29%3C%2Fi%3E%3A+17+pp.+%3Ca+href%3D%22https%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2016.02026%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2016.02026%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftvliz https://doi.org/10.3389/fmicb.2016.02026 2022-05-01T10:54:11Z Microbial life in exposed terrestrial surface layers in continental Antarctica is faced with extreme environmental conditions, including scarcity of organic matter. Bacteria in these exposed settings can therefore be expected to use alternative energy sources such as solar energy, abundant during the austral summer. Using Illumina MiSeq sequencing, we assessed the diversity and abundance of four conserved protein encoding genes involved in different key steps of light-harvesting pathways dependent on (bacterio)chlorophyll (pufM, bchL/chlL, and bchX genes) and rhodopsins (actinorhodopsin genes), in exposed soils from the Sør Rondane Mountains, East Antarctica. Analysis of pufM genes, encoding a subunit of the type 2 photochemical reaction center found in anoxygenic phototrophic bacteria, revealed a broad diversity, dominated by Roseobacter- and Loktanella-like sequences. The bchL and chlL, involved in (bacterio)chlorophyll synthesis, on the other hand, showed a high relative abundance of either cyanobacterial or green algal trebouxiophyceael chlL reads, depending on the sample, while most bchX sequences belonged mostly to previously unidentified phylotypes. Rhodopsin-containing phototrophic bacteria could not be detected in the samples. Our results, while suggesting that Cyanobacteria and green algae are the main phototrophic groups, show that light-harvesting bacteria are nevertheless very diverse in microbial communities in Antarctic soils. Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) Antarctic Austral East Antarctica Sør Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) Sør-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) Frontiers in Microbiology 7 |
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Open Polar |
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Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
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ftvliz |
language |
English |
description |
Microbial life in exposed terrestrial surface layers in continental Antarctica is faced with extreme environmental conditions, including scarcity of organic matter. Bacteria in these exposed settings can therefore be expected to use alternative energy sources such as solar energy, abundant during the austral summer. Using Illumina MiSeq sequencing, we assessed the diversity and abundance of four conserved protein encoding genes involved in different key steps of light-harvesting pathways dependent on (bacterio)chlorophyll (pufM, bchL/chlL, and bchX genes) and rhodopsins (actinorhodopsin genes), in exposed soils from the Sør Rondane Mountains, East Antarctica. Analysis of pufM genes, encoding a subunit of the type 2 photochemical reaction center found in anoxygenic phototrophic bacteria, revealed a broad diversity, dominated by Roseobacter- and Loktanella-like sequences. The bchL and chlL, involved in (bacterio)chlorophyll synthesis, on the other hand, showed a high relative abundance of either cyanobacterial or green algal trebouxiophyceael chlL reads, depending on the sample, while most bchX sequences belonged mostly to previously unidentified phylotypes. Rhodopsin-containing phototrophic bacteria could not be detected in the samples. Our results, while suggesting that Cyanobacteria and green algae are the main phototrophic groups, show that light-harvesting bacteria are nevertheless very diverse in microbial communities in Antarctic soils. |
format |
Article in Journal/Newspaper |
author |
Tahon, G. Tytgat, B. Willems, A. |
spellingShingle |
Tahon, G. Tytgat, B. Willems, A. Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica |
author_facet |
Tahon, G. Tytgat, B. Willems, A. |
author_sort |
Tahon, G. |
title |
Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica |
title_short |
Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica |
title_full |
Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica |
title_fullStr |
Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica |
title_full_unstemmed |
Diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the Sør Rondane Mountains, East Antarctica |
title_sort |
diversity of phototrophic genes suggests multiple bacteria may be able to exploit sunlight in exposed soils from the sør rondane mountains, east antarctica |
publishDate |
2016 |
url |
https://www.vliz.be/imisdocs/publications/305690.pdf |
long_lat |
ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) |
geographic |
Antarctic Austral East Antarctica Sør Rondane Mountains Sør-Rondane |
geographic_facet |
Antarctic Austral East Antarctica Sør Rondane Mountains Sør-Rondane |
genre |
Antarc* Antarctic Antarctica East Antarctica |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica |
op_source |
%3Ci%3EFront.+Microbiol.+7%282026%29%3C%2Fi%3E%3A+17+pp.+%3Ca+href%3D%22https%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2016.02026%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2016.02026%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000389952900001 info:eu-repo/semantics/altIdentifier/doi/.org/10.3389/fmicb.2016.02026 https://www.vliz.be/imisdocs/publications/305690.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2016.02026 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
_version_ |
1766259853622247424 |