Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes
Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long-term adaptive and plastic responses of marine invertebrates to p CO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three p CO 2 r...
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Language: | English |
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ftvliz:oai:oma.vliz.be:284074 2023-05-15T17:50:00+02:00 Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes De Wit, P. Dupont, S. Thorne, P. 2015 application/pdf https://www.vliz.be/imisdocs/publications/300518.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000387763200007 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1111/eva.12335 https://www.vliz.be/imisdocs/publications/300518.pdf info:eu-repo/semantics/openAccess %3Ci%3EEvol.+Appl.+9%289%29%3C%2Fi%3E%3A+1112-1123.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1111%2Feva.12335%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1111%2Feva.12335%3C%2Fa%3E Acclimation Evolution Gene expression Transcription Pseudocalanus info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2015 ftvliz https://doi.org/10.1111/eva.12335 2022-05-01T10:48:42Z Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long-term adaptive and plastic responses of marine invertebrates to p CO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three p CO 2 regimes (400, 900, and 1550 µatm) for two generations, after which we conducted a reciprocal transplant experiment. A de novo transcriptome was assembled, annotated, and gene expression data revealed that genes involved in RNA transcription were strongly down-regulated in populations with long-term exposure to a high p CO 2 environment, even after transplantation back to control levels. In addition, 747 000 SNPs were identified, out of which 1513 showed consistent changes in nucleotide frequency between replicates of control and high p CO 2 populations. Functions involving RNA transcription and ribosomal function, as well as ion transport and oxidative phosphorylation, were highly overrepresented. We thus conclude that p CO 2 stress appears to impose selection in copepods on RNA synthesis and translation, possibly modulated by helicase expression. Using a physiological hypothesis-testing strategy to mine gene expression data, we herein increase the power to detect cellular targets of ocean acidification. This novel approach seems promising for future studies of effects of environmental changes in ecologically important nonmodel organisms. Article in Journal/Newspaper Ocean acidification Copepods Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) Evolutionary Applications 9 9 1112 1123 |
institution |
Open Polar |
collection |
Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
op_collection_id |
ftvliz |
language |
English |
topic |
Acclimation Evolution Gene expression Transcription Pseudocalanus |
spellingShingle |
Acclimation Evolution Gene expression Transcription Pseudocalanus De Wit, P. Dupont, S. Thorne, P. Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
topic_facet |
Acclimation Evolution Gene expression Transcription Pseudocalanus |
description |
Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long-term adaptive and plastic responses of marine invertebrates to p CO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three p CO 2 regimes (400, 900, and 1550 µatm) for two generations, after which we conducted a reciprocal transplant experiment. A de novo transcriptome was assembled, annotated, and gene expression data revealed that genes involved in RNA transcription were strongly down-regulated in populations with long-term exposure to a high p CO 2 environment, even after transplantation back to control levels. In addition, 747 000 SNPs were identified, out of which 1513 showed consistent changes in nucleotide frequency between replicates of control and high p CO 2 populations. Functions involving RNA transcription and ribosomal function, as well as ion transport and oxidative phosphorylation, were highly overrepresented. We thus conclude that p CO 2 stress appears to impose selection in copepods on RNA synthesis and translation, possibly modulated by helicase expression. Using a physiological hypothesis-testing strategy to mine gene expression data, we herein increase the power to detect cellular targets of ocean acidification. This novel approach seems promising for future studies of effects of environmental changes in ecologically important nonmodel organisms. |
format |
Article in Journal/Newspaper |
author |
De Wit, P. Dupont, S. Thorne, P. |
author_facet |
De Wit, P. Dupont, S. Thorne, P. |
author_sort |
De Wit, P. |
title |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_short |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_full |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_fullStr |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_full_unstemmed |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_sort |
selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod pseudocalanus acuspes |
publishDate |
2015 |
url |
https://www.vliz.be/imisdocs/publications/300518.pdf |
genre |
Ocean acidification Copepods |
genre_facet |
Ocean acidification Copepods |
op_source |
%3Ci%3EEvol.+Appl.+9%289%29%3C%2Fi%3E%3A+1112-1123.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1111%2Feva.12335%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1111%2Feva.12335%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000387763200007 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1111/eva.12335 https://www.vliz.be/imisdocs/publications/300518.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/eva.12335 |
container_title |
Evolutionary Applications |
container_volume |
9 |
container_issue |
9 |
container_start_page |
1112 |
op_container_end_page |
1123 |
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1766156568365105152 |