Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas )
Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus , having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B ( gyrB ), RNA pol...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Language: | English |
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2005
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ftvliz:oai:oma.vliz.be:280919 2023-05-15T15:58:35+02:00 Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) Le Roux, F. Goubet, A. Thompson, F.L. Faury, N. Gay, M. Swings, J. Saulnier, D. 2005 application/pdf https://www.vliz.be/imisdocs/publications/296097.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000233349200003 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1099/ijs.0.63666-0 https://www.vliz.be/imisdocs/publications/296097.pdf info:eu-repo/semantics/openAccess %3Ci%3EInt.+J.+Syst.+Evol.+Microbiol.+55%3C%2Fi%3E%3A+2251-2255.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1099%2Fijs.0.63666-0%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1099%2Fijs.0.63666-0%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2005 ftvliz https://doi.org/10.1099/ijs.0.63666-0 2022-05-01T10:45:03Z Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus , having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B ( gyrB ), RNA polymerase σ 70 factor ( rpoD ), replication origin-binding protein ( rctB ) and transmembrane regulatory protein ( toxR ) genes, fluorescent amplified fragment length polymorphism and DNA–DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that differentiate V. gigantis from other known Vibrio species include arginine dihydrolase, gelatinase and β -galactosidase activities, NO 2 production, growth at 35 °C, and utilization of sucrose, melibiose, amygdalin, glycerol, galactose, starch and glycogen. The type strain is LGP 13 T (=LMG 22741 T =CIP 108656 T ). Article in Journal/Newspaper Crassostrea gigas Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) International Journal of Systematic and Evolutionary Microbiology 55 6 2251 2255 |
institution |
Open Polar |
collection |
Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
op_collection_id |
ftvliz |
language |
English |
description |
Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus , having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B ( gyrB ), RNA polymerase σ 70 factor ( rpoD ), replication origin-binding protein ( rctB ) and transmembrane regulatory protein ( toxR ) genes, fluorescent amplified fragment length polymorphism and DNA–DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that differentiate V. gigantis from other known Vibrio species include arginine dihydrolase, gelatinase and β -galactosidase activities, NO 2 production, growth at 35 °C, and utilization of sucrose, melibiose, amygdalin, glycerol, galactose, starch and glycogen. The type strain is LGP 13 T (=LMG 22741 T =CIP 108656 T ). |
format |
Article in Journal/Newspaper |
author |
Le Roux, F. Goubet, A. Thompson, F.L. Faury, N. Gay, M. Swings, J. Saulnier, D. |
spellingShingle |
Le Roux, F. Goubet, A. Thompson, F.L. Faury, N. Gay, M. Swings, J. Saulnier, D. Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) |
author_facet |
Le Roux, F. Goubet, A. Thompson, F.L. Faury, N. Gay, M. Swings, J. Saulnier, D. |
author_sort |
Le Roux, F. |
title |
Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) |
title_short |
Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) |
title_full |
Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) |
title_fullStr |
Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) |
title_full_unstemmed |
Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( Crassostrea gigas ) |
title_sort |
vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters ( crassostrea gigas ) |
publishDate |
2005 |
url |
https://www.vliz.be/imisdocs/publications/296097.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
%3Ci%3EInt.+J.+Syst.+Evol.+Microbiol.+55%3C%2Fi%3E%3A+2251-2255.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1099%2Fijs.0.63666-0%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1099%2Fijs.0.63666-0%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000233349200003 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1099/ijs.0.63666-0 https://www.vliz.be/imisdocs/publications/296097.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1099/ijs.0.63666-0 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
55 |
container_issue |
6 |
container_start_page |
2251 |
op_container_end_page |
2255 |
_version_ |
1766394352400072704 |