Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast
Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induce...
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ftvliz:oai:oma.vliz.be:257164 2023-05-15T16:33:27+02:00 Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast Imazaki, P.H. Brose, F. Jauniaux, T. Das, K. Muller, M. Scippo, M.-L. 2015 application/pdf https://www.vliz.be/imisdocs/publications/292204.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000355443900001 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1155/2015/438295 https://www.vliz.be/imisdocs/publications/292204.pdf info:eu-repo/semantics/openAccess %3Ci%3EBiomed.+Res.+Int.+2015%28438295%29%3C%2Fi%3E%3A+14+pp.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1155%2F2015%2F438295%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1155%2F2015%2F438295%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2015 ftvliz https://doi.org/10.1155/2015/438295 2022-10-19T22:24:36Z Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p'-DDT was the most potent xenoestrogen from the DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only beta-HCH (at 400 and 2000 ng/mL) and delta-HCH (at 2000 ng/mL) displayed estrogenic activities. These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 x 10(2)similar to 4.2 x 10(4) mu g/kg), p,p'-DDE (5.1 x 10(2)similar to 8.6 x 10(3) mu g/kg), and HCB (7.6 x 10(1)similar to 1.5 x 10(3) mu g/kg) were the compounds found in highest concentrations. The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. Article in Journal/Newspaper Harbour porpoise Phocoena phocoena Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) BioMed Research International 2015 1 13 |
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Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
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ftvliz |
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English |
description |
Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p'-DDT was the most potent xenoestrogen from the DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only beta-HCH (at 400 and 2000 ng/mL) and delta-HCH (at 2000 ng/mL) displayed estrogenic activities. These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 x 10(2)similar to 4.2 x 10(4) mu g/kg), p,p'-DDE (5.1 x 10(2)similar to 8.6 x 10(3) mu g/kg), and HCB (7.6 x 10(1)similar to 1.5 x 10(3) mu g/kg) were the compounds found in highest concentrations. The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. |
format |
Article in Journal/Newspaper |
author |
Imazaki, P.H. Brose, F. Jauniaux, T. Das, K. Muller, M. Scippo, M.-L. |
spellingShingle |
Imazaki, P.H. Brose, F. Jauniaux, T. Das, K. Muller, M. Scippo, M.-L. Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast |
author_facet |
Imazaki, P.H. Brose, F. Jauniaux, T. Das, K. Muller, M. Scippo, M.-L. |
author_sort |
Imazaki, P.H. |
title |
Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast |
title_short |
Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast |
title_full |
Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast |
title_fullStr |
Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast |
title_full_unstemmed |
Estrogenic evaluation and organochlorine identification in blubber of North Sea Harbour Porpoise ( Phocoena phocoena ) stranded on the North Sea coast |
title_sort |
estrogenic evaluation and organochlorine identification in blubber of north sea harbour porpoise ( phocoena phocoena ) stranded on the north sea coast |
publishDate |
2015 |
url |
https://www.vliz.be/imisdocs/publications/292204.pdf |
genre |
Harbour porpoise Phocoena phocoena |
genre_facet |
Harbour porpoise Phocoena phocoena |
op_source |
%3Ci%3EBiomed.+Res.+Int.+2015%28438295%29%3C%2Fi%3E%3A+14+pp.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1155%2F2015%2F438295%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1155%2F2015%2F438295%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000355443900001 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1155/2015/438295 https://www.vliz.be/imisdocs/publications/292204.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1155/2015/438295 |
container_title |
BioMed Research International |
container_volume |
2015 |
container_start_page |
1 |
op_container_end_page |
13 |
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1766023133647601664 |