Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts
We have developed a protocol for simultaneous detection of BrdU and PCNA on paraffin sections of teleost tissues and have tested it successfully on dental and orofacial tissues of Atlantic salmon ( Salmo salar ) and zebrafish ( Danio rerio ). By combining decalcification of the tissue, appropriate a...
Published in: | Journal of Applied Ichthyology |
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Language: | English |
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2012
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ftvliz:oai:oma.vliz.be:239259 2023-05-15T15:32:08+02:00 Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts Vandenplas, S. Willems, M. Huysseune, A. 2012 http://www.vliz.be/nl/open-marien-archief?module=ref&refid=239259 en eng info:eu-repo/semantics/altIdentifier/wos/000304251800007 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1111/j.1439-0426.2012.02003.x http://www.vliz.be/nl/open-marien-archief?module=ref&refid=239259 info:eu-repo/semantics/restrictedAccess %3Ci%3EJ.+Appl.+Ichthyol.+28%283%29%3C%2Fi%3E%3A+336-340.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1439-0426.2012.02003.x%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1439-0426.2012.02003.x%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2012 ftvliz https://doi.org/10.1111/j.1439-0426.2012.02003.x 2022-05-01T10:17:38Z We have developed a protocol for simultaneous detection of BrdU and PCNA on paraffin sections of teleost tissues and have tested it successfully on dental and orofacial tissues of Atlantic salmon ( Salmo salar ) and zebrafish ( Danio rerio ). By combining decalcification of the tissue, appropriate antigen retrieval steps, and high quality tissue sections, this protocol allows to study the instantaneous pattern of proliferating cells, as well as cell tracing in large specimens. The technique is therefore highly suitable to study skeletal and dental tissues, tissues that are traditionally not easily accessible to molecular and cellular techniques. Dual BrdU-PCNA immunostaining may serve as a useful tool in experimental studies on fish developmental or regenerative processes such as tissue turnover in orofacial epithelia, continuous tooth replacement, and in the identification of putative stem cells for which the cycling state of BrdU label retaining cells needs to be determined. Article in Journal/Newspaper Atlantic salmon Salmo salar Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) Journal of Applied Ichthyology 28 3 336 340 |
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Open Polar |
collection |
Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
op_collection_id |
ftvliz |
language |
English |
description |
We have developed a protocol for simultaneous detection of BrdU and PCNA on paraffin sections of teleost tissues and have tested it successfully on dental and orofacial tissues of Atlantic salmon ( Salmo salar ) and zebrafish ( Danio rerio ). By combining decalcification of the tissue, appropriate antigen retrieval steps, and high quality tissue sections, this protocol allows to study the instantaneous pattern of proliferating cells, as well as cell tracing in large specimens. The technique is therefore highly suitable to study skeletal and dental tissues, tissues that are traditionally not easily accessible to molecular and cellular techniques. Dual BrdU-PCNA immunostaining may serve as a useful tool in experimental studies on fish developmental or regenerative processes such as tissue turnover in orofacial epithelia, continuous tooth replacement, and in the identification of putative stem cells for which the cycling state of BrdU label retaining cells needs to be determined. |
format |
Article in Journal/Newspaper |
author |
Vandenplas, S. Willems, M. Huysseune, A. |
spellingShingle |
Vandenplas, S. Willems, M. Huysseune, A. Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
author_facet |
Vandenplas, S. Willems, M. Huysseune, A. |
author_sort |
Vandenplas, S. |
title |
Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
title_short |
Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
title_full |
Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
title_fullStr |
Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
title_full_unstemmed |
Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
title_sort |
dual brdu-pcna immunodetection of proliferative cells in dental and orofacial tissues of teleosts |
publishDate |
2012 |
url |
http://www.vliz.be/nl/open-marien-archief?module=ref&refid=239259 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
%3Ci%3EJ.+Appl.+Ichthyol.+28%283%29%3C%2Fi%3E%3A+336-340.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1439-0426.2012.02003.x%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1439-0426.2012.02003.x%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000304251800007 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1111/j.1439-0426.2012.02003.x http://www.vliz.be/nl/open-marien-archief?module=ref&refid=239259 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1439-0426.2012.02003.x |
container_title |
Journal of Applied Ichthyology |
container_volume |
28 |
container_issue |
3 |
container_start_page |
336 |
op_container_end_page |
340 |
_version_ |
1766362634412621824 |