Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla )
A European eel ( Anguilla anguilla ) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected...
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Language: | English |
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ftvliz:oai:oma.vliz.be:130019 2023-05-15T13:26:03+02:00 Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) Pujolar, J.M. Maes, G.E. Van Houdt, J.K.J. Zane, L. 2009 http://www.vliz.be/nl/open-marien-archief?module=ref&refid=130019 en eng info:eu-repo/semantics/altIdentifier/wos/000262678900053 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1111/j.1755-0998.2008.02419.x http://www.vliz.be/nl/open-marien-archief?module=ref&refid=130019 info:eu-repo/semantics/restrictedAccess %3Ci%3EMol.+Ecol.+Resour.+9%281%29%3C%2Fi%3E%3A+233-235.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1755-0998.2008.02419.x%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1755-0998.2008.02419.x%3C%2Fa%3E Microsatellites Sequences Anguilla anguilla info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2009 ftvliz https://doi.org/10.1111/j.1755-0998.2008.02419.x 2022-05-01T09:13:45Z A European eel ( Anguilla anguilla ) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel ( Anguilla rostrata ). Article in Journal/Newspaper Anguilla anguilla Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) Molecular Ecology Resources 9 1 233 235 |
institution |
Open Polar |
collection |
Flanders Marine Institute (VLIZ): Open Marine Archive (OMA) |
op_collection_id |
ftvliz |
language |
English |
topic |
Microsatellites Sequences Anguilla anguilla |
spellingShingle |
Microsatellites Sequences Anguilla anguilla Pujolar, J.M. Maes, G.E. Van Houdt, J.K.J. Zane, L. Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) |
topic_facet |
Microsatellites Sequences Anguilla anguilla |
description |
A European eel ( Anguilla anguilla ) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel ( Anguilla rostrata ). |
format |
Article in Journal/Newspaper |
author |
Pujolar, J.M. Maes, G.E. Van Houdt, J.K.J. Zane, L. |
author_facet |
Pujolar, J.M. Maes, G.E. Van Houdt, J.K.J. Zane, L. |
author_sort |
Pujolar, J.M. |
title |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_short |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_full |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_fullStr |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_full_unstemmed |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_sort |
isolation and characterization of expressed sequence tag-linked microsatellite loci for the european eel ( anguilla anguilla ) |
publishDate |
2009 |
url |
http://www.vliz.be/nl/open-marien-archief?module=ref&refid=130019 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
%3Ci%3EMol.+Ecol.+Resour.+9%281%29%3C%2Fi%3E%3A+233-235.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1755-0998.2008.02419.x%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1755-0998.2008.02419.x%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000262678900053 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1111/j.1755-0998.2008.02419.x http://www.vliz.be/nl/open-marien-archief?module=ref&refid=130019 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1755-0998.2008.02419.x |
container_title |
Molecular Ecology Resources |
container_volume |
9 |
container_issue |
1 |
container_start_page |
233 |
op_container_end_page |
235 |
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1766389765753536512 |