Isolation of psychrophilic halophiles from the Antarctic polar desert

• Main Author: Hall, Caleb Litteljohn
• Other Authors: Bacteriology
• Format: Thesis
• Language: English
• Published: Virginia Polytechnic Institute 1968
• Subjects: LD5655.V855 1968.H33; Antarc*; Antarctic; Antarctica; McMurdo Sound; polar desert
• Online Access: http://hdl.handle.net/10919/64764

Saline soils in the Dry Valley region of McMurdo Sound, Antarctica, contained bacteria showing salt tolerance or requirement. Soils were plated by the spread plate method on soil extract-peptone-yeast extract media to which sodium chloride had been added in concentrations from 0 to15 per cent (w/v). Bacteria isolated from these media at 2 C, 5 C, and 15 C were predominantly Gram negative rods with few Gram positive rods and cocci. No filamentous fungi or Halobacterium spp. were observed on the media used. At 15 C there were no isolates from media containing greater than 15 percent added salt; however, counts of 1.4 x 10 colonies per gram of soil were found at this concentration. As the incubation temperatures were lowered, salt tolerance was lowered. The data indicate that the limited soil microflora observed in saline soils and ponds may be attributed to a combination of low maximal summer temperature and high salinity. Master of Science