Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts /
Fish parvalbumins are heat-stable calcium-binding proteins that are highly cross-reactive in causing allergy symptoms in fish-sensitized patients. The reactivities of parvalbumin-specific monoclonal or polyclonal antibodies with parvalbumins of different fish species allowed their application for de...
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2023
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ftvilniusuniv:oai:vu.lt:elaba:181925133 2024-09-15T17:55:27+00:00 Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / Sližienė, Aistė, Plečkaitytė, Milda, Rudokas, Vytautas, Juškaitė, Karolina, Žvirblis, Gintautas, Žvirblienė, Aurelija 2023 application/pdf https://repository.vu.lt/VU:ELABAPDB181925133&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2023.01.001 https://epublications.vu.lt/object/elaba:181925133/181925133.pdf https://repository.vu.lt/VU:ELABAPDB181925133&prefLang=en_US info:eu-repo/semantics/openAccess Molecular immunology., Oxford : Elsevier Ltd, 2023, vol. 154, p. 80-95. ISSN 0161-5890 eISSN 1872-9142 allergen extracts cross-reactivity fish allergy monoclonal antibodies parvalbumin info:eu-repo/semantics/article 2023 ftvilniusuniv https://doi.org/10.1016/j.molimm.2023.01.001 2024-06-24T14:26:24Z Fish parvalbumins are heat-stable calcium-binding proteins that are highly cross-reactive in causing allergy symptoms in fish-sensitized patients. The reactivities of parvalbumin-specific monoclonal or polyclonal antibodies with parvalbumins of different fish species allowed their application for development of various immunoassays for allergen identification in fish samples. In this study, monoclonal antibodies (MAbs) were generated against two parvalbumins – natural Atlantic cod parvalbumin and recombinant common carp β-parvalbumin expressed in E. coli. Large collections of recombinant parvalbumins and natural allergen extracts of different fish species and other animals were used to identify the specificities of these MAbs using ELISA, Western blot, and dot blot. MAbs demonstrated different patterns of cross-reactivities with recombinant parvalbumins. Their binding affinities were affected by the addition and removal of Ca2+ ions. Moreover, all MAbs showed a broad reactivity with the target antigens in natural fish, chicken, and pork extracts. The ability of two MAbs (clones 7B2 and 3F6) to identify and isolate native parvalbumins from allergen extracts was confirmed by Western blot. Epitope mapping using recombinant fragments of Atlantic cod parvalbumin (Gad m 1) and common carp parvalbumin (Cyp c 1) revealed that 4 out of 5 MAbs recognize parvalbumin regions that contain calcium binding sites. In conclusion, the generated broadly reactive well-characterized MAbs against fish β-parvalbumins could be applied for investigation of parvalbumins of fish and other animals and their detection in allergen extracts. Article in Journal/Newspaper atlantic cod Vilnius University Virtual Library (VU VL) Molecular Immunology 154 80 95 |
institution |
Open Polar |
collection |
Vilnius University Virtual Library (VU VL) |
op_collection_id |
ftvilniusuniv |
language |
English |
topic |
allergen extracts cross-reactivity fish allergy monoclonal antibodies parvalbumin |
spellingShingle |
allergen extracts cross-reactivity fish allergy monoclonal antibodies parvalbumin Sližienė, Aistė, Plečkaitytė, Milda, Rudokas, Vytautas, Juškaitė, Karolina, Žvirblis, Gintautas, Žvirblienė, Aurelija Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
topic_facet |
allergen extracts cross-reactivity fish allergy monoclonal antibodies parvalbumin |
description |
Fish parvalbumins are heat-stable calcium-binding proteins that are highly cross-reactive in causing allergy symptoms in fish-sensitized patients. The reactivities of parvalbumin-specific monoclonal or polyclonal antibodies with parvalbumins of different fish species allowed their application for development of various immunoassays for allergen identification in fish samples. In this study, monoclonal antibodies (MAbs) were generated against two parvalbumins – natural Atlantic cod parvalbumin and recombinant common carp β-parvalbumin expressed in E. coli. Large collections of recombinant parvalbumins and natural allergen extracts of different fish species and other animals were used to identify the specificities of these MAbs using ELISA, Western blot, and dot blot. MAbs demonstrated different patterns of cross-reactivities with recombinant parvalbumins. Their binding affinities were affected by the addition and removal of Ca2+ ions. Moreover, all MAbs showed a broad reactivity with the target antigens in natural fish, chicken, and pork extracts. The ability of two MAbs (clones 7B2 and 3F6) to identify and isolate native parvalbumins from allergen extracts was confirmed by Western blot. Epitope mapping using recombinant fragments of Atlantic cod parvalbumin (Gad m 1) and common carp parvalbumin (Cyp c 1) revealed that 4 out of 5 MAbs recognize parvalbumin regions that contain calcium binding sites. In conclusion, the generated broadly reactive well-characterized MAbs against fish β-parvalbumins could be applied for investigation of parvalbumins of fish and other animals and their detection in allergen extracts. |
format |
Article in Journal/Newspaper |
author |
Sližienė, Aistė, Plečkaitytė, Milda, Rudokas, Vytautas, Juškaitė, Karolina, Žvirblis, Gintautas, Žvirblienė, Aurelija |
author_facet |
Sližienė, Aistė, Plečkaitytė, Milda, Rudokas, Vytautas, Juškaitė, Karolina, Žvirblis, Gintautas, Žvirblienė, Aurelija |
author_sort |
Sližienė, Aistė, |
title |
Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
title_short |
Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
title_full |
Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
title_fullStr |
Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
title_full_unstemmed |
Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
title_sort |
cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts / |
publishDate |
2023 |
url |
https://repository.vu.lt/VU:ELABAPDB181925133&prefLang=en_US |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
Molecular immunology., Oxford : Elsevier Ltd, 2023, vol. 154, p. 80-95. ISSN 0161-5890 eISSN 1872-9142 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2023.01.001 https://epublications.vu.lt/object/elaba:181925133/181925133.pdf https://repository.vu.lt/VU:ELABAPDB181925133&prefLang=en_US |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.molimm.2023.01.001 |
container_title |
Molecular Immunology |
container_volume |
154 |
container_start_page |
80 |
op_container_end_page |
95 |
_version_ |
1810431742603952128 |