Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe
Hantaviruses are zoonotic pathogens that can cause subclinical to lethal infections in humans. In Europe, five orthohantaviruses are present in rodents: Myodes-associated Puumala orthohantavirus (PUUV), Microtus-associated Tula orthohantavirus, Traemmersee hantavirus (TRAV)/ Tatenale hantavirus (TAT...
Published in: | Infection, Genetics and Evolution |
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ftvilniusuniv:oai:elaba:76791661 2023-05-15T17:12:39+02:00 Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe Drewes, Stephan Jeske, Kathrin Straková, Petra Balčiauskas, Linas Ryll, René Balčiauskienė, Laima Kohlhause, David Schnidrig, Guy-Alain Hiltbrunner, Melanie Špakova, Aliona Insodaitė, Rasa Petraitytė-Burneikienė, Rasa Heckel, Gerald Ulrich, Rainer G. 2020 application/pdf https://repository.vu.lt/VU:ELABAPDB76791661&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2020.104520 https://epublications.vu.lt/object/elaba:76791661/76791661.pdf https://repository.vu.lt/VU:ELABAPDB76791661&prefLang=en_US info:eu-repo/semantics/openAccess Infection, genetics and evolution, Amsterdam : Elsevier B.V., 2021, vol. 90, art. no. 104520, p. [1-10] ISSN 1567-1348 eISSN 1567-7257 Microtus oeconomus Lithuania Reservoir host Tatenale hantavirus Traemmersee hantavirus Rusne hantavirus Hybrid sequence capture Genome sequence info:eu-repo/semantics/article 2020 ftvilniusuniv https://doi.org/10.1016/j.meegid.2020.104520 2021-12-16T00:12:47Z Hantaviruses are zoonotic pathogens that can cause subclinical to lethal infections in humans. In Europe, five orthohantaviruses are present in rodents: Myodes-associated Puumala orthohantavirus (PUUV), Microtus-associated Tula orthohantavirus, Traemmersee hantavirus (TRAV)/ Tatenale hantavirus (TATV)/ Kielder hantavirus, rat-borne Seoul orthohantavirus, and Apodemus-associated Dobrava-Belgrade orthohantavirus (DOBV). Human PUUV and DOBV infections were detected previously in Lithuania, but the presence of Microtus-associated hantaviruses is not known. For this study we screened 234 Microtus voles, including root voles (Microtus oeconomus), field voles (Microtus agrestis) and common voles (Microtus arvalis) from Lithuania for hantavirus infections. This initial screening was based on reverse transcription-polymerase chain reaction (RT-PCR) targeting the S segment and serological analysis. A novel hantavirus was detected in eight of 79 root voles tentatively named “Rusne virus” according to the capture location and complete genome sequences were determined. In the coding regions of all three genome segments, Rusne virus showed high sequence similarity to TRAV and TATV and clustered with Kielder hantavirus in phylogenetic analyses of partial S and L segment sequences. Pairwise evolutionary distance analysis confirmed Rusne virus as a strain of the species TRAV/TATV. Moreover, we synthesized the entire nucleocapsid (N) protein of Rusne virus in Saccharomyces cerevisiae. We observed cross-reactivity of antibodies raised against other hantaviruses, including PUUV, with this new N protein. ELISA investigation of all 234 voles detected Rusne virus-reactive antibodies exclusively in four of 79 root voles, all being also RNA positive, but not in any other vole species. In conclusion, the detection of Rusne virus RNA in multiple root voles at the same trapping site during three years and its absence in sympatric field voles suggests root voles as the reservoir host of this novel virus. Future investigations should evaluate host association of TRAV, TATV, Kielder virus and the novel Rusne virus and their evolutionary relationships. Article in Journal/Newspaper Microtus arvalis Vilnius University Virtual Library (VU VL) Tula ENVELOPE(-65.650,-65.650,-65.517,-65.517) Infection, Genetics and Evolution 90 104520 |
institution |
Open Polar |
collection |
Vilnius University Virtual Library (VU VL) |
op_collection_id |
ftvilniusuniv |
language |
English |
topic |
Microtus oeconomus Lithuania Reservoir host Tatenale hantavirus Traemmersee hantavirus Rusne hantavirus Hybrid sequence capture Genome sequence |
spellingShingle |
Microtus oeconomus Lithuania Reservoir host Tatenale hantavirus Traemmersee hantavirus Rusne hantavirus Hybrid sequence capture Genome sequence Drewes, Stephan Jeske, Kathrin Straková, Petra Balčiauskas, Linas Ryll, René Balčiauskienė, Laima Kohlhause, David Schnidrig, Guy-Alain Hiltbrunner, Melanie Špakova, Aliona Insodaitė, Rasa Petraitytė-Burneikienė, Rasa Heckel, Gerald Ulrich, Rainer G. Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe |
topic_facet |
Microtus oeconomus Lithuania Reservoir host Tatenale hantavirus Traemmersee hantavirus Rusne hantavirus Hybrid sequence capture Genome sequence |
description |
Hantaviruses are zoonotic pathogens that can cause subclinical to lethal infections in humans. In Europe, five orthohantaviruses are present in rodents: Myodes-associated Puumala orthohantavirus (PUUV), Microtus-associated Tula orthohantavirus, Traemmersee hantavirus (TRAV)/ Tatenale hantavirus (TATV)/ Kielder hantavirus, rat-borne Seoul orthohantavirus, and Apodemus-associated Dobrava-Belgrade orthohantavirus (DOBV). Human PUUV and DOBV infections were detected previously in Lithuania, but the presence of Microtus-associated hantaviruses is not known. For this study we screened 234 Microtus voles, including root voles (Microtus oeconomus), field voles (Microtus agrestis) and common voles (Microtus arvalis) from Lithuania for hantavirus infections. This initial screening was based on reverse transcription-polymerase chain reaction (RT-PCR) targeting the S segment and serological analysis. A novel hantavirus was detected in eight of 79 root voles tentatively named “Rusne virus” according to the capture location and complete genome sequences were determined. In the coding regions of all three genome segments, Rusne virus showed high sequence similarity to TRAV and TATV and clustered with Kielder hantavirus in phylogenetic analyses of partial S and L segment sequences. Pairwise evolutionary distance analysis confirmed Rusne virus as a strain of the species TRAV/TATV. Moreover, we synthesized the entire nucleocapsid (N) protein of Rusne virus in Saccharomyces cerevisiae. We observed cross-reactivity of antibodies raised against other hantaviruses, including PUUV, with this new N protein. ELISA investigation of all 234 voles detected Rusne virus-reactive antibodies exclusively in four of 79 root voles, all being also RNA positive, but not in any other vole species. In conclusion, the detection of Rusne virus RNA in multiple root voles at the same trapping site during three years and its absence in sympatric field voles suggests root voles as the reservoir host of this novel virus. Future investigations should evaluate host association of TRAV, TATV, Kielder virus and the novel Rusne virus and their evolutionary relationships. |
format |
Article in Journal/Newspaper |
author |
Drewes, Stephan Jeske, Kathrin Straková, Petra Balčiauskas, Linas Ryll, René Balčiauskienė, Laima Kohlhause, David Schnidrig, Guy-Alain Hiltbrunner, Melanie Špakova, Aliona Insodaitė, Rasa Petraitytė-Burneikienė, Rasa Heckel, Gerald Ulrich, Rainer G. |
author_facet |
Drewes, Stephan Jeske, Kathrin Straková, Petra Balčiauskas, Linas Ryll, René Balčiauskienė, Laima Kohlhause, David Schnidrig, Guy-Alain Hiltbrunner, Melanie Špakova, Aliona Insodaitė, Rasa Petraitytė-Burneikienė, Rasa Heckel, Gerald Ulrich, Rainer G. |
author_sort |
Drewes, Stephan |
title |
Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe |
title_short |
Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe |
title_full |
Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe |
title_fullStr |
Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe |
title_full_unstemmed |
Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe |
title_sort |
identification of a novel hantavirus strain in the root vole (microtus oeconomus) in lithuania, eastern europe |
publishDate |
2020 |
url |
https://repository.vu.lt/VU:ELABAPDB76791661&prefLang=en_US |
long_lat |
ENVELOPE(-65.650,-65.650,-65.517,-65.517) |
geographic |
Tula |
geographic_facet |
Tula |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Infection, genetics and evolution, Amsterdam : Elsevier B.V., 2021, vol. 90, art. no. 104520, p. [1-10] ISSN 1567-1348 eISSN 1567-7257 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2020.104520 https://epublications.vu.lt/object/elaba:76791661/76791661.pdf https://repository.vu.lt/VU:ELABAPDB76791661&prefLang=en_US |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.meegid.2020.104520 |
container_title |
Infection, Genetics and Evolution |
container_volume |
90 |
container_start_page |
104520 |
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1766069439840649216 |