Lipase B from Candida antarctica — the wide applicable biocatalyst in obtaining pharmaceutical compounds

• Published in: Medical Research Journal
• Main Authors: Dulęba, Jacek, Czirson, Katarzyna, Siódmiak, Tomasz, Marszałł, Michał Piotr
• Language: English
• Published: Via Medica 2019
• Subjects: lipase B from Candida antarctica; immobilization; triglycerides; interfacial activation; kinetic resolution; transesterification; acylation; Antarc*; Antarctica
• Online Access: https://journals.viamedica.pl/medical_research_journal/article/view/MRJ.a2019.0030; https://doi.org/10.5603/MRJ.a2019.0030

Lipases are commonly applied in the pharmaceutical and chemical industry, especially in immobilizedform. The use of immobilized lipases facilitates the design of reactors and control of reactions, for example,fast stopping the reaction. The immobilization procedure should increase the stability of the lipaseand its activity, as well as be simple and efficient. Lipase B from Candida antarctica (CAL-B) is an enzymefrom the lipase group, isolated from the Candida antarctica species. CAL-B has the highest activity innon-polar organic solvents, such as hexane and toluene, and the lowest in polar solvents, e.g. acetonitrile.Due to its hydrolytic properties, this enzyme degrades triglycerides of fatty acids to free fatty acids (FFA)and glycerol. Described lipase is often immobilized, in the aim to increase enantioselective and lipolyticactivity. The kinetic and dynamic resolution with the application of lipase is one of the ways in obtainingan enantiopure form of the drugs, which usually are more effective and safer for the patient. The CAL-Bcould be also applied in the kinetic resolution of compounds being building blocks, derivates of drugs orconjugated forms. Furthermore, the CAL-B is used in the reactions in receiving of organic compounds,which are the natural origin, especially vegetable. Based on the presented data, it can be concluded, thatCAL-B is an enzyme with a wide application in the biosynthesis of compounds with therapeutic activity.