Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 com...

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Published in:Folia Histochemica et Cytobiologica
Main Authors: Czykier, Elzbieta, Zabel, Maciej, Seidel, Jolanta
Published: Via Medica 2010
Subjects:
Common vole
Microtus arvalis
Online Access:https://journals.viamedica.pl/folia_histochemica_cytobiologica/article/view/4228
https://doi.org/10.2478/v10042-010-0003-5
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spelling ftviamedicaojs:oai:czasopisma.viamedica.pl:article/4228 2023-05-15T15:56:36+02:00 Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice. Czykier, Elzbieta Zabel, Maciej Seidel, Jolanta 2010-08-03 application/pdf https://journals.viamedica.pl/folia_histochemica_cytobiologica/article/view/4228 https://doi.org/10.2478/v10042-010-0003-5 Via Medica By sending the manuscript with figures and charts the author (authors) declares (declare) it has been neither published nor submitted to the Editorial Board of any other journal (not including the abstracts 400 words or less). Moreover, it is assumed that the copyright for publishing and distribution of the submitted material (in all known now and developed in the future forms and fields of exploitation) are automatically and free-of-charge transferred to the Polish Histochemical and Cytochemical Society, under condition that those materials are accepted for publication. The authors accept the fact, that the work will not be published anywhere and in any language without the prior written consent of the owner of the copyrights, i.e. the Polish Histochemical and Cytochemical Society. Independently to the formerly mentioned effects, author (authors) due to release form the responsibilities to the third parties, shall (should) attach the cover letter to the manuscript. The cover letter shall contain declaration of the author (authors) confirming formerly described facts and that all co-authors of the manuscript agree for the publication. Moreover the author (authors) confirm that they have acquaint themselves with the editorial policy in “Folia Histochemica et Cytobiologica” and they oblige to respect the policy. The reward for sending the manuscript is publication in the journal. The written permission from the previous Polish Histochemical and Cytochemical Society for the reprint should be enclosed to the materials (pictures, photos, figures, etc.) that have been printed previously. If the data presented in case reports, figures or in the text enable identification of the persons their written consent to the publication should be enclosed. Any potential conflict of interest will be dealt with by the local court specific to the Polish Histochemical and Cytochemical Society. Legal relations between the Polish Histochemical and Cytochemical Society and the author(s) are Folia Histochemica et Cytobiologica; Vol 48, No 2 (2010); 306-310 2010 ftviamedicaojs https://doi.org/10.2478/v10042-010-0003-5 2019-03-25T12:20:35Z The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini. Other/Unknown Material Common vole Microtus arvalis Via Medica Journals Folia Histochemica et Cytobiologica 48 2
institution Open Polar
collection Via Medica Journals
op_collection_id ftviamedicaojs
description The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini.
author Czykier, Elzbieta
Zabel, Maciej
Seidel, Jolanta
spellingShingle Czykier, Elzbieta
Zabel, Maciej
Seidel, Jolanta
Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.
author_facet Czykier, Elzbieta
Zabel, Maciej
Seidel, Jolanta
author_sort Czykier, Elzbieta
title Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.
title_short Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.
title_full Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.
title_fullStr Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.
title_full_unstemmed Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.
title_sort immunolocalization of pthrp in the parotid glands of three rodents species: clethrionomys glareoulus, microtus arvalis and white swiss mice.
publisher Via Medica
publishDate 2010
url https://journals.viamedica.pl/folia_histochemica_cytobiologica/article/view/4228
https://doi.org/10.2478/v10042-010-0003-5
genre Common vole
Microtus arvalis
genre_facet Common vole
Microtus arvalis
op_source Folia Histochemica et Cytobiologica; Vol 48, No 2 (2010); 306-310
op_rights By sending the manuscript with figures and charts the author (authors) declares (declare) it has been neither published nor submitted to the Editorial Board of any other journal (not including the abstracts 400 words or less). Moreover, it is assumed that the copyright for publishing and distribution of the submitted material (in all known now and developed in the future forms and fields of exploitation) are automatically and free-of-charge transferred to the Polish Histochemical and Cytochemical Society, under condition that those materials are accepted for publication. The authors accept the fact, that the work will not be published anywhere and in any language without the prior written consent of the owner of the copyrights, i.e. the Polish Histochemical and Cytochemical Society. Independently to the formerly mentioned effects, author (authors) due to release form the responsibilities to the third parties, shall (should) attach the cover letter to the manuscript. The cover letter shall contain declaration of the author (authors) confirming formerly described facts and that all co-authors of the manuscript agree for the publication. Moreover the author (authors) confirm that they have acquaint themselves with the editorial policy in “Folia Histochemica et Cytobiologica” and they oblige to respect the policy. The reward for sending the manuscript is publication in the journal. The written permission from the previous Polish Histochemical and Cytochemical Society for the reprint should be enclosed to the materials (pictures, photos, figures, etc.) that have been printed previously. If the data presented in case reports, figures or in the text enable identification of the persons their written consent to the publication should be enclosed. Any potential conflict of interest will be dealt with by the local court specific to the Polish Histochemical and Cytochemical Society. Legal relations between the Polish Histochemical and Cytochemical Society and the author(s) are
op_doi https://doi.org/10.2478/v10042-010-0003-5
container_title Folia Histochemica et Cytobiologica
container_volume 48
container_issue 2
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