An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls
Xylan and cellulose are abundant polysaccharides in vascular plants and essential for secondary cell wall strength. Acetate or glucuronic acid decorations are exclusively found on even-numbered residues in most of the glucuronoxylan polymer. It has been proposed that this even-specific positioning o...
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ftuwarwick:oai:wrap.warwick.ac.uk:94864 2023-05-15T16:06:31+02:00 An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand-Tardif, Mylene Brown, Steven P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul 2017-11 application/pdf http://wrap.warwick.ac.uk/94864/ http://wrap.warwick.ac.uk/94864/1/WRAP-pattern-xylan-substitution-critical-interaction-cellulose-plant-cell-walls-Dupree-2017.pdf https://doi.org/10.1038/s41477-017-0030-8 unknown Nature Publishing Group http://wrap.warwick.ac.uk/94864/1/WRAP-pattern-xylan-substitution-critical-interaction-cellulose-plant-cell-walls-Dupree-2017.pdf Grantham, Nicholas J., Wurman-Rodrich, Joel, Terrett, Oliver M., Lyczakowski, Jan J., Stott, Katherine, Iuga, Dinu, Simmons, Thomas J., Durand-Tardif, Mylene, Brown, Steven P., Dupree, Ray, Busse-Wicher, Marta and Dupree, Paul (2017) An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls. Nature Plants, 3 (11). pp. 859-865. doi:10.1038/s41477-017-0030-8 <http://dx.doi.org/10.1038/s41477-017-0030-8> QK Botany Journal Article NonPeerReviewed 2017 ftuwarwick https://doi.org/10.1038/s41477-017-0030-8 2022-03-16T21:19:26Z Xylan and cellulose are abundant polysaccharides in vascular plants and essential for secondary cell wall strength. Acetate or glucuronic acid decorations are exclusively found on even-numbered residues in most of the glucuronoxylan polymer. It has been proposed that this even-specific positioning of the decorations might permit docking of xylan onto the hydrophilic face of a cellulose microfibril1,2,3. Consequently, xylan adopts a flattened ribbon-like twofold screw conformation when bound to cellulose in the cell wall4. Here we show that ESKIMO1/XOAT1/TBL29, a xylan-specific O-acetyltransferase, is necessary for generation of the even pattern of acetyl esters on xylan in Arabidopsis. The reduced acetylation in the esk1 mutant deregulates the position-specific activity of the xylan glucuronosyltransferase GUX1, and so the even pattern of glucuronic acid on the xylan is lost. Solid-state NMR of intact cell walls shows that, without the even-patterned xylan decorations, xylan does not interact normally with cellulose fibrils. We conclude that the even pattern of xylan substitutions seen across vascular plants enables the interaction of xylan with hydrophilic faces of cellulose fibrils, and is essential for development of normal plant secondary cell walls. Article in Journal/Newspaper eskimo* The University of Warwick: WRAP - Warwick Research Archive Portal Nature Plants 3 11 859 865 |
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The University of Warwick: WRAP - Warwick Research Archive Portal |
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QK Botany Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand-Tardif, Mylene Brown, Steven P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
topic_facet |
QK Botany |
description |
Xylan and cellulose are abundant polysaccharides in vascular plants and essential for secondary cell wall strength. Acetate or glucuronic acid decorations are exclusively found on even-numbered residues in most of the glucuronoxylan polymer. It has been proposed that this even-specific positioning of the decorations might permit docking of xylan onto the hydrophilic face of a cellulose microfibril1,2,3. Consequently, xylan adopts a flattened ribbon-like twofold screw conformation when bound to cellulose in the cell wall4. Here we show that ESKIMO1/XOAT1/TBL29, a xylan-specific O-acetyltransferase, is necessary for generation of the even pattern of acetyl esters on xylan in Arabidopsis. The reduced acetylation in the esk1 mutant deregulates the position-specific activity of the xylan glucuronosyltransferase GUX1, and so the even pattern of glucuronic acid on the xylan is lost. Solid-state NMR of intact cell walls shows that, without the even-patterned xylan decorations, xylan does not interact normally with cellulose fibrils. We conclude that the even pattern of xylan substitutions seen across vascular plants enables the interaction of xylan with hydrophilic faces of cellulose fibrils, and is essential for development of normal plant secondary cell walls. |
format |
Article in Journal/Newspaper |
author |
Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand-Tardif, Mylene Brown, Steven P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul |
author_facet |
Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand-Tardif, Mylene Brown, Steven P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul |
author_sort |
Grantham, Nicholas J. |
title |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_short |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_full |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_fullStr |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_full_unstemmed |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_sort |
even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
publisher |
Nature Publishing Group |
publishDate |
2017 |
url |
http://wrap.warwick.ac.uk/94864/ http://wrap.warwick.ac.uk/94864/1/WRAP-pattern-xylan-substitution-critical-interaction-cellulose-plant-cell-walls-Dupree-2017.pdf https://doi.org/10.1038/s41477-017-0030-8 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_relation |
http://wrap.warwick.ac.uk/94864/1/WRAP-pattern-xylan-substitution-critical-interaction-cellulose-plant-cell-walls-Dupree-2017.pdf Grantham, Nicholas J., Wurman-Rodrich, Joel, Terrett, Oliver M., Lyczakowski, Jan J., Stott, Katherine, Iuga, Dinu, Simmons, Thomas J., Durand-Tardif, Mylene, Brown, Steven P., Dupree, Ray, Busse-Wicher, Marta and Dupree, Paul (2017) An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls. Nature Plants, 3 (11). pp. 859-865. doi:10.1038/s41477-017-0030-8 <http://dx.doi.org/10.1038/s41477-017-0030-8> |
op_doi |
https://doi.org/10.1038/s41477-017-0030-8 |
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Nature Plants |
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3 |
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11 |
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859 |
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865 |
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1766402444268404736 |