Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H
Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccha...
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ftuwarwick:oai:wrap.warwick.ac.uk:86088 2023-05-15T15:05:00+02:00 Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H Casillo, Angela Ståhle, Jonas Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Pieretti, Giuseppina Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Widmalm, Göran Tutino, Maria L. Corsaro, Maria M. 2017-11 application/pdf http://wrap.warwick.ac.uk/86088/ http://wrap.warwick.ac.uk/86088/1/WRAP_Structural_characterization.pdf https://doi.org/10.1007/s10482-017-0834-6 unknown Springer http://wrap.warwick.ac.uk/86088/1/WRAP_Structural_characterization.pdf Casillo, Angela, Ståhle, Jonas, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Pieretti, Giuseppina, Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parrilli, Michelangelo, Widmalm, Göran, Tutino, Maria L. and Corsaro, Maria M. (2017) Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H. Antonie van Leeuwenhoek, 110 (11). pp. 1377-1387. doi:10.1007/s10482-017-0834-6 <http://dx.doi.org/10.1007/s10482-017-0834-6> QD Chemistry Journal Article NonPeerReviewed 2017 ftuwarwick https://doi.org/10.1007/s10482-017-0834-6 2022-03-16T21:09:45Z Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: →4)-β-d-GlcpNAcA-(1 →3)-β-d-QuipNAc4NAc-(1 →3)-β-d-GalpNAc-(1 →. The 3D model, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 °C, this molecule displays only a low ice recrystallization inhibition activity. Article in Journal/Newspaper Arctic The University of Warwick: WRAP - Warwick Research Archive Portal Arctic Antonie van Leeuwenhoek 110 11 1377 1387 |
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The University of Warwick: WRAP - Warwick Research Archive Portal |
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ftuwarwick |
language |
unknown |
topic |
QD Chemistry |
spellingShingle |
QD Chemistry Casillo, Angela Ståhle, Jonas Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Pieretti, Giuseppina Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Widmalm, Göran Tutino, Maria L. Corsaro, Maria M. Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H |
topic_facet |
QD Chemistry |
description |
Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: →4)-β-d-GlcpNAcA-(1 →3)-β-d-QuipNAc4NAc-(1 →3)-β-d-GalpNAc-(1 →. The 3D model, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 °C, this molecule displays only a low ice recrystallization inhibition activity. |
format |
Article in Journal/Newspaper |
author |
Casillo, Angela Ståhle, Jonas Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Pieretti, Giuseppina Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Widmalm, Göran Tutino, Maria L. Corsaro, Maria M. |
author_facet |
Casillo, Angela Ståhle, Jonas Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Pieretti, Giuseppina Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Widmalm, Göran Tutino, Maria L. Corsaro, Maria M. |
author_sort |
Casillo, Angela |
title |
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H |
title_short |
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H |
title_full |
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H |
title_fullStr |
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H |
title_full_unstemmed |
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H |
title_sort |
structural characterization of an all-aminosugar-containing capsular polysaccharide from colwellia psychrerythraea 34h |
publisher |
Springer |
publishDate |
2017 |
url |
http://wrap.warwick.ac.uk/86088/ http://wrap.warwick.ac.uk/86088/1/WRAP_Structural_characterization.pdf https://doi.org/10.1007/s10482-017-0834-6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://wrap.warwick.ac.uk/86088/1/WRAP_Structural_characterization.pdf Casillo, Angela, Ståhle, Jonas, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Pieretti, Giuseppina, Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parrilli, Michelangelo, Widmalm, Göran, Tutino, Maria L. and Corsaro, Maria M. (2017) Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H. Antonie van Leeuwenhoek, 110 (11). pp. 1377-1387. doi:10.1007/s10482-017-0834-6 <http://dx.doi.org/10.1007/s10482-017-0834-6> |
op_doi |
https://doi.org/10.1007/s10482-017-0834-6 |
container_title |
Antonie van Leeuwenhoek |
container_volume |
110 |
container_issue |
11 |
container_start_page |
1377 |
op_container_end_page |
1387 |
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1766336765604397056 |