Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection
Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides...
Published in: | Carbohydrate Polymers |
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Pergamon Press
2017
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Online Access: | http://wrap.warwick.ac.uk/81596/ http://wrap.warwick.ac.uk/81596/3/WRAP_carbpol-d-16-02223_manuscript.pdf https://doi.org/10.1016/j.carbpol.2016.09.037 |
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ftuwarwick:oai:wrap.warwick.ac.uk:81596 2023-05-15T18:18:22+02:00 Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria Corsaro, Michela 2017-01-20 application/pdf http://wrap.warwick.ac.uk/81596/ http://wrap.warwick.ac.uk/81596/3/WRAP_carbpol-d-16-02223_manuscript.pdf https://doi.org/10.1016/j.carbpol.2016.09.037 unknown Pergamon Press http://wrap.warwick.ac.uk/81596/3/WRAP_carbpol-d-16-02223_manuscript.pdf Casillo, Angela, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parilli, Michelangelo, Cosconati, Sandro, Novellino, Ettore, Randazzo, Antonio, Tutino, Maria and Corsaro, Michela (2017) Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection. Carbohydrate Polymers, 156 . pp. 364-371. doi:10.1016/j.carbpol.2016.09.037 <http://dx.doi.org/10.1016/j.carbpol.2016.09.037> QH Natural history Journal Article NonPeerReviewed 2017 ftuwarwick https://doi.org/10.1016/j.carbpol.2016.09.037 2022-03-16T21:07:45Z Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides with cryoprotection activity. We here describe the purification and detailed molecular primary and secondary structure of the exopolysaccharide (EPS) secreted by C. psychrerythraea 34H cells grown at 4°C. The structure was determined by chemical analysis and NMR. The trisaccharide repeating unit of the EPS is constituted by a N-acetyl quinovosamine unit and two residues of galacturonic acid both decorated with alanine. In addition, the EPS was tested in vitro showing a significant inhibitory effect on ice recrystallization. In-depth NMR and computational analysis suggest a pseudohelicoidal structure which seems to prevent the local tetrahedral order of the water molecules in the first hydration shell, and could be responsible of the inhibition of ice recrystallization. As cell cryopreservation is an essential tool in modern biotechnology and medicine, the observations reported in this paper could pave the way for a biotechnological application of Colwellia EPS. Article in Journal/Newspaper Sea ice The University of Warwick: WRAP - Warwick Research Archive Portal Carbohydrate Polymers 156 364 371 |
institution |
Open Polar |
collection |
The University of Warwick: WRAP - Warwick Research Archive Portal |
op_collection_id |
ftuwarwick |
language |
unknown |
topic |
QH Natural history |
spellingShingle |
QH Natural history Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria Corsaro, Michela Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
topic_facet |
QH Natural history |
description |
Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides with cryoprotection activity. We here describe the purification and detailed molecular primary and secondary structure of the exopolysaccharide (EPS) secreted by C. psychrerythraea 34H cells grown at 4°C. The structure was determined by chemical analysis and NMR. The trisaccharide repeating unit of the EPS is constituted by a N-acetyl quinovosamine unit and two residues of galacturonic acid both decorated with alanine. In addition, the EPS was tested in vitro showing a significant inhibitory effect on ice recrystallization. In-depth NMR and computational analysis suggest a pseudohelicoidal structure which seems to prevent the local tetrahedral order of the water molecules in the first hydration shell, and could be responsible of the inhibition of ice recrystallization. As cell cryopreservation is an essential tool in modern biotechnology and medicine, the observations reported in this paper could pave the way for a biotechnological application of Colwellia EPS. |
format |
Article in Journal/Newspaper |
author |
Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria Corsaro, Michela |
author_facet |
Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria Corsaro, Michela |
author_sort |
Casillo, Angela |
title |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
title_short |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
title_full |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
title_fullStr |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
title_full_unstemmed |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
title_sort |
structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection |
publisher |
Pergamon Press |
publishDate |
2017 |
url |
http://wrap.warwick.ac.uk/81596/ http://wrap.warwick.ac.uk/81596/3/WRAP_carbpol-d-16-02223_manuscript.pdf https://doi.org/10.1016/j.carbpol.2016.09.037 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_relation |
http://wrap.warwick.ac.uk/81596/3/WRAP_carbpol-d-16-02223_manuscript.pdf Casillo, Angela, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parilli, Michelangelo, Cosconati, Sandro, Novellino, Ettore, Randazzo, Antonio, Tutino, Maria and Corsaro, Michela (2017) Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection. Carbohydrate Polymers, 156 . pp. 364-371. doi:10.1016/j.carbpol.2016.09.037 <http://dx.doi.org/10.1016/j.carbpol.2016.09.037> |
op_doi |
https://doi.org/10.1016/j.carbpol.2016.09.037 |
container_title |
Carbohydrate Polymers |
container_volume |
156 |
container_start_page |
364 |
op_container_end_page |
371 |
_version_ |
1766194916908597248 |