Aggregation and fibril morphology of the arctic mutation of Alzheimer’s Aβ peptide by CD, TEM, STEM and in situ AFM

• Published in: Journal of Structural Biology
• Main Authors: Norlin, Nils, Hellberg, Magnus, Filippov, Andrei, Sousa, Alioscka A., Gröbner, Gerhard, Leapman, Richard D., Almqvist, Nils, Antzutkin, Oleg N.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Academic Press 2012
• Subjects: Arctic
• Online Access: http://wrap.warwick.ac.uk/52140/; https://doi.org/10.1016/j.jsb.2012.06.010

Morphology of aggregation intermediates, polymorphism of amyloid fibrils and aggregation kinetics of the "Arctic" mutant of the Alzheimer's amyloid beta-peptide, A beta((1-40))(E22G), in a physiologically relevant Tris buffer (pH 7.4) were thoroughly explored in comparison with the human wild type Alzheimer's amyloid peptide, wt-A beta((1-40)), using both in situ atomic force and electron microscopy, circular dichroism and thioflavin T fluorescence assays. For arc-A beta((1-40)) at the end of the 'lag'-period of fibrillization an abrupt appearance of similar to 3 nm size 'spherical aggregates' with a homogeneous morphology, was identified. Then, the aggregation proceeds with a rapid growth of amyloid fibrils with a variety of morphologies, while the spherical aggregates eventually disappeared during in situ measurements. Arc-A beta((1-40)) was also shown to form fibrils at much lower concentrations than wt-A beta((1-40)): <= 2.5 mu M and 12.5 mu M, respectively. Moreover, at the same concentration, 50 mu M, the aggregation process proceeds more rapidly for arc-A beta((1-40)): the first amyloid fibrils were observed after Ca. 72 h from the onset of incubation as compared to approximately 7 days for wt-A beta((1-40)). Amyloid fibrils of arc-A beta((1-40)) exhibit a large variety of polymorphs, at least five, both coiled and non-coiled distinct fibril structures were recognized by AFM, while at least four types of arc-A beta((1-40)) fibrils were identified by TEM and STEM and their mass-per-length statistics were collected suggesting supramolecular structures with two, four and six beta-sheet laminae. Our results suggest a pathway of fibrillogenesis for full-length Alzheimer's peptides with small and structurally ordered transient spherical aggregates as on-pathway immediate precursors of amyloid fibrils. (C) 2012 Elsevier Inc. All rights reserved