Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization...
Published in: | Biomacromolecules |
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American Chemical Society
2021
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Online Access: | http://wrap.warwick.ac.uk/150494/ http://wrap.warwick.ac.uk/150494/7/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf http://wrap.warwick.ac.uk/150494/1/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf https://doi.org/10.1021/acs.biomac.0c01659 |
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ftuwarwick:oai:wrap.warwick.ac.uk:150494 2023-05-15T16:37:50+02:00 Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides Casillo, Angela Fabozzi, Antonio Russo Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousai Radulescu, Aurel Tutino, Maria L. Paduano, Luigi Corsaro, Maria M. 2021-04-12 application/pdf http://wrap.warwick.ac.uk/150494/ http://wrap.warwick.ac.uk/150494/7/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf http://wrap.warwick.ac.uk/150494/1/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf https://doi.org/10.1021/acs.biomac.0c01659 unknown American Chemical Society http://wrap.warwick.ac.uk/150494/7/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf http://wrap.warwick.ac.uk/150494/1/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf Casillo, Angela, Fabozzi, Antonio, Russo Krauss, Irene, Parrilli, Ermenegilda, Biggs, Caroline I., Gibson, Matthew I., Lanzetta, Rosa, Appavou, Marie-Sousai, Radulescu, Aurel, Tutino, Maria L., Paduano, Luigi and Corsaro, Maria M. (2021) Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides. Biomacromolecules, 22 (4). pp. 1445-1457. doi:10.1021/acs.biomac.0c01659 <http://dx.doi.org/10.1021/acs.biomac.0c01659> ISSN 1525-7797. QC Physics QR Microbiology Journal Article NonPeerReviewed 2021 ftuwarwick https://doi.org/10.1021/acs.biomac.0c01659 2023-03-16T23:42:15Z Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation. Article in Journal/Newspaper Ice permafrost The University of Warwick: WRAP - Warwick Research Archive Portal Biomacromolecules 22 4 1445 1457 |
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The University of Warwick: WRAP - Warwick Research Archive Portal |
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ftuwarwick |
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unknown |
topic |
QC Physics QR Microbiology |
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QC Physics QR Microbiology Casillo, Angela Fabozzi, Antonio Russo Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousai Radulescu, Aurel Tutino, Maria L. Paduano, Luigi Corsaro, Maria M. Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
topic_facet |
QC Physics QR Microbiology |
description |
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation. |
format |
Article in Journal/Newspaper |
author |
Casillo, Angela Fabozzi, Antonio Russo Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousai Radulescu, Aurel Tutino, Maria L. Paduano, Luigi Corsaro, Maria M. |
author_facet |
Casillo, Angela Fabozzi, Antonio Russo Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousai Radulescu, Aurel Tutino, Maria L. Paduano, Luigi Corsaro, Maria M. |
author_sort |
Casillo, Angela |
title |
Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
title_short |
Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
title_full |
Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
title_fullStr |
Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
title_full_unstemmed |
Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
title_sort |
physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides |
publisher |
American Chemical Society |
publishDate |
2021 |
url |
http://wrap.warwick.ac.uk/150494/ http://wrap.warwick.ac.uk/150494/7/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf http://wrap.warwick.ac.uk/150494/1/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf https://doi.org/10.1021/acs.biomac.0c01659 |
genre |
Ice permafrost |
genre_facet |
Ice permafrost |
op_relation |
http://wrap.warwick.ac.uk/150494/7/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf http://wrap.warwick.ac.uk/150494/1/WRAP-physicochemical-approach-understanding-structure-conformation-activity-mannan-polysaccharides-Gibson-2021.pdf Casillo, Angela, Fabozzi, Antonio, Russo Krauss, Irene, Parrilli, Ermenegilda, Biggs, Caroline I., Gibson, Matthew I., Lanzetta, Rosa, Appavou, Marie-Sousai, Radulescu, Aurel, Tutino, Maria L., Paduano, Luigi and Corsaro, Maria M. (2021) Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides. Biomacromolecules, 22 (4). pp. 1445-1457. doi:10.1021/acs.biomac.0c01659 <http://dx.doi.org/10.1021/acs.biomac.0c01659> ISSN 1525-7797. |
op_doi |
https://doi.org/10.1021/acs.biomac.0c01659 |
container_title |
Biomacromolecules |
container_volume |
22 |
container_issue |
4 |
container_start_page |
1445 |
op_container_end_page |
1457 |
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1766028135477805056 |