Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature
Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated P...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , , , , , , , , , , , |
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2021
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Online Access: | https://research.bangor.ac.uk/portal/en/researchoutputs/seaice-bacteria-halomonas-sp-strain-363-and-paracoccus-sp-strain-392-produce-multiple-types-of-poly3hydroxyalkaonoic-acid-pha-storage-polymers-at-low-temperature(cd51e1ee-caa2-441c-8d65-0d85ff37ca40).html https://doi.org/10.1128/AEM.00929-21 https://research.bangor.ac.uk/ws/files/40883150/AEM.00929_21.pdf |
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ftuwalesbangcris:oai:research.bangor.ac.uk:publications/cd51e1ee-caa2-441c-8d65-0d85ff37ca40 2023-05-15T18:17:22+02:00 Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature Eronen-Rasimus, E. Hultman, Jenni Hai, Tran Pessi, L.S. Wright, Samuel Laine, P. Viitamaki, S. Lyra, C. Thomas, D.N. Golyshin, Peter Luhtanen, A.M. Kuosa, H. Kaartokallio, H. 2021-08-11 application/pdf https://research.bangor.ac.uk/portal/en/researchoutputs/seaice-bacteria-halomonas-sp-strain-363-and-paracoccus-sp-strain-392-produce-multiple-types-of-poly3hydroxyalkaonoic-acid-pha-storage-polymers-at-low-temperature(cd51e1ee-caa2-441c-8d65-0d85ff37ca40).html https://doi.org/10.1128/AEM.00929-21 https://research.bangor.ac.uk/ws/files/40883150/AEM.00929_21.pdf eng eng info:eu-repo/semantics/openAccess Eronen-Rasimus , E , Hultman , J , Hai , T , Pessi , L S , Wright , S , Laine , P , Viitamaki , S , Lyra , C , Thomas , D N , Golyshin , P , Luhtanen , A M , Kuosa , H & Kaartokallio , H 2021 , ' Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature ' , Applied and Environmental Microbiology , vol. 87 , no. 17 , e00929-21 . https://doi.org/10.1128/AEM.00929-21 Halomonas MCL-PHA PHA Paracoccus SCL-PHA copolymer genomics marine bacteria poly-3-hydroxyalkanoic acid sea-ice bacteria transcriptomics article 2021 ftuwalesbangcris https://doi.org/10.1128/AEM.00929-21 2022-03-07T10:37:36Z Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy, and chromatography. We show that both bacterial strains produce PHAs at 4°C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 carries three phaC synthase genes and transcribes genes along three PHA pathways (I to III), whereas Paracoccus sp. 392 carries only one phaC gene and transcribes genes along one pathway (I). Thus, Halomonas sp. 363 has a versatile repertoire of phaC genes and pathways enabling production of both short- and medium-chain-length PHA products. IMPORTANCE Plastic pollution is one of the most topical threats to the health of the oceans and seas. One recognized way to alleviate the problem is to use degradable bioplastic materials in high-risk applications. PHA is a promising bioplastic material as it is nontoxic and fully produced and degraded by bacteria. Sea ice is an interesting environment for prospecting novel PHA-producing organisms, since traits advantageous to lower production costs, such as tolerance for high salinities and low temperatures, are common. We show that two sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, are able to produce various types of PHA from inexpensive carbon sources. Halomonas sp. 363 is an especially interesting PHA-producing organism, since it has three different synthesis pathways to produce both short- and medium-chain-length PHAs. Article in Journal/Newspaper Sea ice Southern Ocean Bangor University: Research Portal Southern Ocean Applied and Environmental Microbiology 87 17 |
institution |
Open Polar |
collection |
Bangor University: Research Portal |
op_collection_id |
ftuwalesbangcris |
language |
English |
topic |
Halomonas MCL-PHA PHA Paracoccus SCL-PHA copolymer genomics marine bacteria poly-3-hydroxyalkanoic acid sea-ice bacteria transcriptomics |
spellingShingle |
Halomonas MCL-PHA PHA Paracoccus SCL-PHA copolymer genomics marine bacteria poly-3-hydroxyalkanoic acid sea-ice bacteria transcriptomics Eronen-Rasimus, E. Hultman, Jenni Hai, Tran Pessi, L.S. Wright, Samuel Laine, P. Viitamaki, S. Lyra, C. Thomas, D.N. Golyshin, Peter Luhtanen, A.M. Kuosa, H. Kaartokallio, H. Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
topic_facet |
Halomonas MCL-PHA PHA Paracoccus SCL-PHA copolymer genomics marine bacteria poly-3-hydroxyalkanoic acid sea-ice bacteria transcriptomics |
description |
Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy, and chromatography. We show that both bacterial strains produce PHAs at 4°C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 carries three phaC synthase genes and transcribes genes along three PHA pathways (I to III), whereas Paracoccus sp. 392 carries only one phaC gene and transcribes genes along one pathway (I). Thus, Halomonas sp. 363 has a versatile repertoire of phaC genes and pathways enabling production of both short- and medium-chain-length PHA products. IMPORTANCE Plastic pollution is one of the most topical threats to the health of the oceans and seas. One recognized way to alleviate the problem is to use degradable bioplastic materials in high-risk applications. PHA is a promising bioplastic material as it is nontoxic and fully produced and degraded by bacteria. Sea ice is an interesting environment for prospecting novel PHA-producing organisms, since traits advantageous to lower production costs, such as tolerance for high salinities and low temperatures, are common. We show that two sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, are able to produce various types of PHA from inexpensive carbon sources. Halomonas sp. 363 is an especially interesting PHA-producing organism, since it has three different synthesis pathways to produce both short- and medium-chain-length PHAs. |
format |
Article in Journal/Newspaper |
author |
Eronen-Rasimus, E. Hultman, Jenni Hai, Tran Pessi, L.S. Wright, Samuel Laine, P. Viitamaki, S. Lyra, C. Thomas, D.N. Golyshin, Peter Luhtanen, A.M. Kuosa, H. Kaartokallio, H. |
author_facet |
Eronen-Rasimus, E. Hultman, Jenni Hai, Tran Pessi, L.S. Wright, Samuel Laine, P. Viitamaki, S. Lyra, C. Thomas, D.N. Golyshin, Peter Luhtanen, A.M. Kuosa, H. Kaartokallio, H. |
author_sort |
Eronen-Rasimus, E. |
title |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_short |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_full |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_fullStr |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_full_unstemmed |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_sort |
sea-ice bacteria halomonas sp. strain 363 and paracoccus sp. strain 392 produce multiple types of poly-3-hydroxyalkaonoic acid (pha) storage polymers at low temperature |
publishDate |
2021 |
url |
https://research.bangor.ac.uk/portal/en/researchoutputs/seaice-bacteria-halomonas-sp-strain-363-and-paracoccus-sp-strain-392-produce-multiple-types-of-poly3hydroxyalkaonoic-acid-pha-storage-polymers-at-low-temperature(cd51e1ee-caa2-441c-8d65-0d85ff37ca40).html https://doi.org/10.1128/AEM.00929-21 https://research.bangor.ac.uk/ws/files/40883150/AEM.00929_21.pdf |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Sea ice Southern Ocean |
genre_facet |
Sea ice Southern Ocean |
op_source |
Eronen-Rasimus , E , Hultman , J , Hai , T , Pessi , L S , Wright , S , Laine , P , Viitamaki , S , Lyra , C , Thomas , D N , Golyshin , P , Luhtanen , A M , Kuosa , H & Kaartokallio , H 2021 , ' Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature ' , Applied and Environmental Microbiology , vol. 87 , no. 17 , e00929-21 . https://doi.org/10.1128/AEM.00929-21 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/AEM.00929-21 |
container_title |
Applied and Environmental Microbiology |
container_volume |
87 |
container_issue |
17 |
_version_ |
1766191556465786880 |