Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species
Infectious diseases represent an important barrier to sustainable aquaculture development. Rearing density can substantially impact fish productivity, health and welfare in aquaculture, including growth rates, behaviour and, crucially, immune activity. Given the current emphasis on aquaculture diver...
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Online Access: | https://research.bangor.ac.uk/portal/en/researchoutputs/comparative-transcriptomics-reveal-conserved-impacts-of-rearing-density-on-immune-response-of-two-important-aquaculture-species(5ad55a0b-b47d-42d2-848f-d6c0ab0bc8b4).html https://research.bangor.ac.uk/ws/files/28983689/2020_Carative_transcriptomics_reveal_conserved_impacts_of_reasrinf_density_on_immune_response_of_two_important_aquaculture_species.pdf |
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ftuwalesbangcris:oai:research.bangor.ac.uk:publications/5ad55a0b-b47d-42d2-848f-d6c0ab0bc8b4 2023-05-15T15:31:39+02:00 Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species Ellison, Amy Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo 2020-09 application/pdf https://research.bangor.ac.uk/portal/en/researchoutputs/comparative-transcriptomics-reveal-conserved-impacts-of-rearing-density-on-immune-response-of-two-important-aquaculture-species(5ad55a0b-b47d-42d2-848f-d6c0ab0bc8b4).html https://research.bangor.ac.uk/ws/files/28983689/2020_Carative_transcriptomics_reveal_conserved_impacts_of_reasrinf_density_on_immune_response_of_two_important_aquaculture_species.pdf eng eng info:eu-repo/semantics/openAccess Ellison , A , Uren Webster , T M , Rodriguez-Barreto , D , Garcia de Leaniz , C , Consuegra , S , Orozco-terWengel , P & Cable , J 2020 , ' Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species ' , Fish and Shellfish Immunology , vol. 104 , pp. 192-201 . Rearing density Stress Immunity Transcriptome comparative transcriptomics Atlantic Salmon Nile tilapia Saprolegnia parasitica Th17 responses article 2020 ftuwalesbangcris 2021-12-26T12:06:48Z Infectious diseases represent an important barrier to sustainable aquaculture development. Rearing density can substantially impact fish productivity, health and welfare in aquaculture, including growth rates, behaviour and, crucially, immune activity. Given the current emphasis on aquaculture diversification, stress-related indicators broadly applicable across species are needed. Utilising an interspecific comparative transcriptomic (RNAseq) approach, we compared gill gene expression responses of Atlantic salmon (Salmo salar) and Nile tilapia (Oreochromis niloticus) to rearing density and Saprolegnia parasitica infection. Salmon reared at high-density showed increased expression of stress-related markers (e.g. c-fos and hsp70), and downregulation of innate immune genes. Upon pathogen challenge, only salmon reared at low density exhibited increased expression of inflammatory interleukins and lymphocyte-related genes. Tilapia immunity, in contrast, was impaired at low-density. Using overlapping gene ontology enrichment and gene ortholog analyses, we found that density-related stress similarly impacted salmon and tilapia in key immune pathways, altering the expression of genes vital to inflammatory and Th17 responses to pathogen challenge. Given the challenges posed by ectoparasites and gill diseases in fish farms, this study underscores the importance of optimal rearing densities for immunocompetence, particularly for mucosal immunity. Our comparative transcriptomics analyses identified density stress impacted immune markers common across different fish taxa, providing key molecular targets with potential for monitoring and enhancing aquaculture resilience in a wide range of farmed species. Article in Journal/Newspaper Atlantic salmon Salmo salar Bangor University: Research Portal |
institution |
Open Polar |
collection |
Bangor University: Research Portal |
op_collection_id |
ftuwalesbangcris |
language |
English |
topic |
Rearing density Stress Immunity Transcriptome comparative transcriptomics Atlantic Salmon Nile tilapia Saprolegnia parasitica Th17 responses |
spellingShingle |
Rearing density Stress Immunity Transcriptome comparative transcriptomics Atlantic Salmon Nile tilapia Saprolegnia parasitica Th17 responses Ellison, Amy Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
topic_facet |
Rearing density Stress Immunity Transcriptome comparative transcriptomics Atlantic Salmon Nile tilapia Saprolegnia parasitica Th17 responses |
description |
Infectious diseases represent an important barrier to sustainable aquaculture development. Rearing density can substantially impact fish productivity, health and welfare in aquaculture, including growth rates, behaviour and, crucially, immune activity. Given the current emphasis on aquaculture diversification, stress-related indicators broadly applicable across species are needed. Utilising an interspecific comparative transcriptomic (RNAseq) approach, we compared gill gene expression responses of Atlantic salmon (Salmo salar) and Nile tilapia (Oreochromis niloticus) to rearing density and Saprolegnia parasitica infection. Salmon reared at high-density showed increased expression of stress-related markers (e.g. c-fos and hsp70), and downregulation of innate immune genes. Upon pathogen challenge, only salmon reared at low density exhibited increased expression of inflammatory interleukins and lymphocyte-related genes. Tilapia immunity, in contrast, was impaired at low-density. Using overlapping gene ontology enrichment and gene ortholog analyses, we found that density-related stress similarly impacted salmon and tilapia in key immune pathways, altering the expression of genes vital to inflammatory and Th17 responses to pathogen challenge. Given the challenges posed by ectoparasites and gill diseases in fish farms, this study underscores the importance of optimal rearing densities for immunocompetence, particularly for mucosal immunity. Our comparative transcriptomics analyses identified density stress impacted immune markers common across different fish taxa, providing key molecular targets with potential for monitoring and enhancing aquaculture resilience in a wide range of farmed species. |
format |
Article in Journal/Newspaper |
author |
Ellison, Amy Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo |
author_facet |
Ellison, Amy Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo |
author_sort |
Ellison, Amy |
title |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_short |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_full |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_fullStr |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_full_unstemmed |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_sort |
comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
publishDate |
2020 |
url |
https://research.bangor.ac.uk/portal/en/researchoutputs/comparative-transcriptomics-reveal-conserved-impacts-of-rearing-density-on-immune-response-of-two-important-aquaculture-species(5ad55a0b-b47d-42d2-848f-d6c0ab0bc8b4).html https://research.bangor.ac.uk/ws/files/28983689/2020_Carative_transcriptomics_reveal_conserved_impacts_of_reasrinf_density_on_immune_response_of_two_important_aquaculture_species.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Ellison , A , Uren Webster , T M , Rodriguez-Barreto , D , Garcia de Leaniz , C , Consuegra , S , Orozco-terWengel , P & Cable , J 2020 , ' Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species ' , Fish and Shellfish Immunology , vol. 104 , pp. 192-201 . |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766362188999557120 |