Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts
Vasostatins (VSs), i.e. the main biologically active peptides generated by the proteolytic processing of chromogranin A (CGA) N-terminus, exert negative inotropism in vertebrate hearts. Here, using isolated working eel (Anguilla anguilla) and frog (Rana esculenta) heart preparations, we have studied...
Published in: | Regulatory Peptides |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2007
|
Subjects: | |
Online Access: | https://hdl.handle.net/20.500.11768/2914 https://doi.org/10.1016/j.regpep.2006.09.002 |
id |
ftuvssanraffaele:oai:iris.unisr.it:20.500.11768/2914 |
---|---|
record_format |
openpolar |
spelling |
ftuvssanraffaele:oai:iris.unisr.it:20.500.11768/2914 2024-02-27T08:33:01+00:00 Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts Mazza R Mannarino C Imbrogno S Barbieri SF Adamo C Angelone T Tota B. CORTI , ANGELO Mazza, R Mannarino, C Imbrogno, S Barbieri, Sf Adamo, C Angelone, T Corti, Angelo Tota, B. 2007 https://hdl.handle.net/20.500.11768/2914 https://doi.org/10.1016/j.regpep.2006.09.002 unknown info:eu-repo/semantics/altIdentifier/wos/WOS:000244012400015 volume:138 issue:2-3 firstpage:145 lastpage:151 numberofpages:7 journal:REGULATORY PEPTIDES http://hdl.handle.net/20.500.11768/2914 doi:10.1016/j.regpep.2006.09.002 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33845994419 info:eu-repo/semantics/article 2007 ftuvssanraffaele https://doi.org/20.500.11768/291410.1016/j.regpep.2006.09.002 2024-01-31T17:39:37Z Vasostatins (VSs), i.e. the main biologically active peptides generated by the proteolytic processing of chromogranin A (CGA) N-terminus, exert negative inotropism in vertebrate hearts. Here, using isolated working eel (Anguilla anguilla) and frog (Rana esculenta) heart preparations, we have studied the role of the cytoskeleton in the VSs-mediated inotropic response. In both eel and frog hearts, VSs-mediated-negative inotropy was abolished by treatment with inhibitors of cytoskeleton reorganization, such as cytochalasin-D (eel: 10 nM; frog: 1 nM), an inhibitor of actin polymerisation, wortmannin (0.01 nM), an inhibitor of PI3-kinase (PI3-K)/protein kinase B (Akt) signal-transduction cascade, butanedione 2-monoxime (BDM) (eel: 100 nM; frog: 10 nM), an antagonist of myosin ATPase, and N-(6-aminohexil)-5-chloro-1-naphthalenesulfonamide (W7) (eel: 100 nM; frog: I nM), a calcium-calmodulin antagonist. These results demonstrate that changes in cytoskeletal dynamics play a crucial role in the negative inotropic influence of VSs on eel and frog hearts. (c) 2006 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Anguilla anguilla IRIS UniSR (Università Vita-Salute San Raffaele) Regulatory Peptides 138 2-3 145 151 |
institution |
Open Polar |
collection |
IRIS UniSR (Università Vita-Salute San Raffaele) |
op_collection_id |
ftuvssanraffaele |
language |
unknown |
description |
Vasostatins (VSs), i.e. the main biologically active peptides generated by the proteolytic processing of chromogranin A (CGA) N-terminus, exert negative inotropism in vertebrate hearts. Here, using isolated working eel (Anguilla anguilla) and frog (Rana esculenta) heart preparations, we have studied the role of the cytoskeleton in the VSs-mediated inotropic response. In both eel and frog hearts, VSs-mediated-negative inotropy was abolished by treatment with inhibitors of cytoskeleton reorganization, such as cytochalasin-D (eel: 10 nM; frog: 1 nM), an inhibitor of actin polymerisation, wortmannin (0.01 nM), an inhibitor of PI3-kinase (PI3-K)/protein kinase B (Akt) signal-transduction cascade, butanedione 2-monoxime (BDM) (eel: 100 nM; frog: 10 nM), an antagonist of myosin ATPase, and N-(6-aminohexil)-5-chloro-1-naphthalenesulfonamide (W7) (eel: 100 nM; frog: I nM), a calcium-calmodulin antagonist. These results demonstrate that changes in cytoskeletal dynamics play a crucial role in the negative inotropic influence of VSs on eel and frog hearts. (c) 2006 Elsevier B.V. All rights reserved. |
author2 |
Mazza, R Mannarino, C Imbrogno, S Barbieri, Sf Adamo, C Angelone, T Corti, Angelo Tota, B. |
format |
Article in Journal/Newspaper |
author |
Mazza R Mannarino C Imbrogno S Barbieri SF Adamo C Angelone T Tota B. CORTI , ANGELO |
spellingShingle |
Mazza R Mannarino C Imbrogno S Barbieri SF Adamo C Angelone T Tota B. CORTI , ANGELO Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts |
author_facet |
Mazza R Mannarino C Imbrogno S Barbieri SF Adamo C Angelone T Tota B. CORTI , ANGELO |
author_sort |
Mazza R |
title |
Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts |
title_short |
Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts |
title_full |
Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts |
title_fullStr |
Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts |
title_full_unstemmed |
Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts |
title_sort |
crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin a-derived peptides in eel and frog hearts |
publishDate |
2007 |
url |
https://hdl.handle.net/20.500.11768/2914 https://doi.org/10.1016/j.regpep.2006.09.002 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000244012400015 volume:138 issue:2-3 firstpage:145 lastpage:151 numberofpages:7 journal:REGULATORY PEPTIDES http://hdl.handle.net/20.500.11768/2914 doi:10.1016/j.regpep.2006.09.002 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33845994419 |
op_doi |
https://doi.org/20.500.11768/291410.1016/j.regpep.2006.09.002 |
container_title |
Regulatory Peptides |
container_volume |
138 |
container_issue |
2-3 |
container_start_page |
145 |
op_container_end_page |
151 |
_version_ |
1792043431425474560 |