Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action
We have studied the effects of exogenous human recombinant Vasostatin-1 (VS-1), Vasostatin-2 (VS-2) and the human Chromogranin A (CGA) 7-57 synthetic peptides on the mechanical performance of the isolated and perfused working eel (Anguilla anguilla) heart. Under basal conditions, the three peptides...
Published in: | General and Comparative Endocrinology |
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ftuvssanraffaele:oai:iris.unisr.it:20.500.11768/2908 2024-02-27T08:33:00+00:00 Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action Imbrogno S Angelone T Adamo C Helle KB Tota B. CORTI , ANGELO Imbrogno, S Angelone, T Corti, Angelo Adamo, C Helle, Kb Tota, B. 2004 https://hdl.handle.net/20.500.11768/2908 https://doi.org/10.1016/j.ygcen.2004.07.008 unknown info:eu-repo/semantics/altIdentifier/wos/WOS:000224722600003 volume:139 issue:1 firstpage:20 lastpage:28 numberofpages:9 journal:GENERAL AND COMPARATIVE ENDOCRINOLOGY http://hdl.handle.net/20.500.11768/2908 doi:10.1016/j.ygcen.2004.07.008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-5144224367 info:eu-repo/semantics/article 2004 ftuvssanraffaele https://doi.org/20.500.11768/290810.1016/j.ygcen.2004.07.008 2024-01-31T17:36:25Z We have studied the effects of exogenous human recombinant Vasostatin-1 (VS-1), Vasostatin-2 (VS-2) and the human Chromogranin A (CGA) 7-57 synthetic peptides on the mechanical performance of the isolated and perfused working eel (Anguilla anguilla) heart. Under basal conditions, the three peptides decreased stroke volume (SV) and stroke work (SW), thus exerting negative inotropism. The VS-1-mediated negative inotropism was abolished by exposure to inhibitors of either Gi/o protein (pertussis toxin; PTx) or M1 muscarinic receptors (Pirenzepine) or calcium (Lantanum and Diltiazem) and potassium (Ba2+, 4-aminopyridine, tetraethylammonium, glibenclamide) channels, while it required an intact endocardial endothelium (EE). Using N-G-monomethyl-L-arginine (L-NMMA) as an inhibitor of nitric oxide (NO) synthase (NOS), and hemoglobin as a NO scavenger, we demonstrated the obligatory role of NO signaling in mediating the vasostatin response. Pretreatment with either a specific inhibitor of soluble guanylate cyclase (GC) 1H-(1,2,4)oxadiazolo-(4,3-alpha)quinoxalin-1-one (ODQ) or the inhibitor of the cGMP-activated protein kinase (PKG) KT5823, abolished the VS-1-mediated inotropism, indicating the cGMP-PKG component as a crucial target of NO signaling. Of note, VS-1 was effective in counteracting the adrenergic (Isoproterenol and Phenylephrine)-mediated positive inotropism. These findings provide the first evidence that vasostatins exert cardiotropic action in fish, thus suggesting their long evolutionary history as well as their species-specific mechanisms of action. (C) 2004 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Anguilla anguilla IRIS UniSR (Università Vita-Salute San Raffaele) General and Comparative Endocrinology 139 1 20 28 |
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Open Polar |
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IRIS UniSR (Università Vita-Salute San Raffaele) |
op_collection_id |
ftuvssanraffaele |
language |
unknown |
description |
We have studied the effects of exogenous human recombinant Vasostatin-1 (VS-1), Vasostatin-2 (VS-2) and the human Chromogranin A (CGA) 7-57 synthetic peptides on the mechanical performance of the isolated and perfused working eel (Anguilla anguilla) heart. Under basal conditions, the three peptides decreased stroke volume (SV) and stroke work (SW), thus exerting negative inotropism. The VS-1-mediated negative inotropism was abolished by exposure to inhibitors of either Gi/o protein (pertussis toxin; PTx) or M1 muscarinic receptors (Pirenzepine) or calcium (Lantanum and Diltiazem) and potassium (Ba2+, 4-aminopyridine, tetraethylammonium, glibenclamide) channels, while it required an intact endocardial endothelium (EE). Using N-G-monomethyl-L-arginine (L-NMMA) as an inhibitor of nitric oxide (NO) synthase (NOS), and hemoglobin as a NO scavenger, we demonstrated the obligatory role of NO signaling in mediating the vasostatin response. Pretreatment with either a specific inhibitor of soluble guanylate cyclase (GC) 1H-(1,2,4)oxadiazolo-(4,3-alpha)quinoxalin-1-one (ODQ) or the inhibitor of the cGMP-activated protein kinase (PKG) KT5823, abolished the VS-1-mediated inotropism, indicating the cGMP-PKG component as a crucial target of NO signaling. Of note, VS-1 was effective in counteracting the adrenergic (Isoproterenol and Phenylephrine)-mediated positive inotropism. These findings provide the first evidence that vasostatins exert cardiotropic action in fish, thus suggesting their long evolutionary history as well as their species-specific mechanisms of action. (C) 2004 Elsevier Inc. All rights reserved. |
author2 |
Imbrogno, S Angelone, T Corti, Angelo Adamo, C Helle, Kb Tota, B. |
format |
Article in Journal/Newspaper |
author |
Imbrogno S Angelone T Adamo C Helle KB Tota B. CORTI , ANGELO |
spellingShingle |
Imbrogno S Angelone T Adamo C Helle KB Tota B. CORTI , ANGELO Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action |
author_facet |
Imbrogno S Angelone T Adamo C Helle KB Tota B. CORTI , ANGELO |
author_sort |
Imbrogno S |
title |
Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action |
title_short |
Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action |
title_full |
Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action |
title_fullStr |
Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action |
title_full_unstemmed |
Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action |
title_sort |
influence of vasostatins, the chromogranin a-derived peptides, on the working heart of the eel (anguilla anguilla): negative inotropy and mechanism of action |
publishDate |
2004 |
url |
https://hdl.handle.net/20.500.11768/2908 https://doi.org/10.1016/j.ygcen.2004.07.008 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000224722600003 volume:139 issue:1 firstpage:20 lastpage:28 numberofpages:9 journal:GENERAL AND COMPARATIVE ENDOCRINOLOGY http://hdl.handle.net/20.500.11768/2908 doi:10.1016/j.ygcen.2004.07.008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-5144224367 |
op_doi |
https://doi.org/20.500.11768/290810.1016/j.ygcen.2004.07.008 |
container_title |
General and Comparative Endocrinology |
container_volume |
139 |
container_issue |
1 |
container_start_page |
20 |
op_container_end_page |
28 |
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1792043291468890112 |