Evolution of three neuropeptides isolated from the brain of sturgeon
In vertebrates the brain superimposes control on fundamental processes such as reproduction and growth. Neuropeptides secreted from the brain initiate a cascade of events that affect these processes. In this thesis three neuropeptides are examined to determine their structures and patterns in the co...
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ftuvicpubl:oai:dspace.library.uvic.ca:1828/9767 2023-05-15T18:19:31+02:00 Evolution of three neuropeptides isolated from the brain of sturgeon Lescheid, David William Sherwood, Nancy 1997 application/pdf https://dspace.library.uvic.ca//handle/1828/9767 English en eng https://dspace.library.uvic.ca//handle/1828/9767 Available to the World Wide Web Fishes Physiology Neuropeptides Pathophysiology Neurobiology Thesis 1997 ftuvicpubl 2022-05-19T06:10:34Z In vertebrates the brain superimposes control on fundamental processes such as reproduction and growth. Neuropeptides secreted from the brain initiate a cascade of events that affect these processes. In this thesis three neuropeptides are examined to determine their structures and patterns in the context of vertebrate evolution. Reproduction in vertebrates is controlled by the neuropeptide gonadotropin-releasing hormone, GnRH, a decapeptide belonging to a peptide family of twelve known members. One common theme in vertebrates is that there is usually more than one form of GnRH in the brain of a single species; often each form of GnRH has a separate location in the brain and therefore, an implied distinct function. In this thesis, the brain of Siberian sturgeon, Acipenser gueldenstaedti, initially was examined for GnRH using reversed-phase high performance liquid chromatography, HPLC, and radioimmunoassay, RIA, with specific antisera and was shown to contain mammalian (m)GnRH by chemical sequence analysis and by accurate determination of the molecular mass. In addition, another form of GnRH, termed chicken (c)GnRH-II, was found in the sturgeon brain. This is the first report to show that the primary structure of GnRH is identical in an evolutionarily-ancient fish and in mammals including humans. Further, the second form of GnRH, cGnRH-11, was identified for the first time in the brain of adult stumptail monkeys (Macaca speciosa) as well as in adult and fetal rhesus monkey (Macaca mulatta) brains. This study implies that at least two forms of GnRH are found in the brain of most vertebrate species including mammals. In cartilaginous fish that evolved earlier than sturgeon, the same HPLC and RIA methods were used to demonstrate that regions of the brain and pituitary of skate. Raja canebensis, also contained cGnRH-II but dogfish (df)GnRH rather than mGnRH. By the same criteria, teleost fish like whitefish (Prosopium williamsoni), platyfish (Xiphophorus maculatus), green swordtail (Xiphophorus hellerei) and sablefish ... Thesis Siberian sturgeon University of Victoria (Canada): UVicDSpace |
institution |
Open Polar |
collection |
University of Victoria (Canada): UVicDSpace |
op_collection_id |
ftuvicpubl |
language |
English |
topic |
Fishes Physiology Neuropeptides Pathophysiology Neurobiology |
spellingShingle |
Fishes Physiology Neuropeptides Pathophysiology Neurobiology Lescheid, David William Evolution of three neuropeptides isolated from the brain of sturgeon |
topic_facet |
Fishes Physiology Neuropeptides Pathophysiology Neurobiology |
description |
In vertebrates the brain superimposes control on fundamental processes such as reproduction and growth. Neuropeptides secreted from the brain initiate a cascade of events that affect these processes. In this thesis three neuropeptides are examined to determine their structures and patterns in the context of vertebrate evolution. Reproduction in vertebrates is controlled by the neuropeptide gonadotropin-releasing hormone, GnRH, a decapeptide belonging to a peptide family of twelve known members. One common theme in vertebrates is that there is usually more than one form of GnRH in the brain of a single species; often each form of GnRH has a separate location in the brain and therefore, an implied distinct function. In this thesis, the brain of Siberian sturgeon, Acipenser gueldenstaedti, initially was examined for GnRH using reversed-phase high performance liquid chromatography, HPLC, and radioimmunoassay, RIA, with specific antisera and was shown to contain mammalian (m)GnRH by chemical sequence analysis and by accurate determination of the molecular mass. In addition, another form of GnRH, termed chicken (c)GnRH-II, was found in the sturgeon brain. This is the first report to show that the primary structure of GnRH is identical in an evolutionarily-ancient fish and in mammals including humans. Further, the second form of GnRH, cGnRH-11, was identified for the first time in the brain of adult stumptail monkeys (Macaca speciosa) as well as in adult and fetal rhesus monkey (Macaca mulatta) brains. This study implies that at least two forms of GnRH are found in the brain of most vertebrate species including mammals. In cartilaginous fish that evolved earlier than sturgeon, the same HPLC and RIA methods were used to demonstrate that regions of the brain and pituitary of skate. Raja canebensis, also contained cGnRH-II but dogfish (df)GnRH rather than mGnRH. By the same criteria, teleost fish like whitefish (Prosopium williamsoni), platyfish (Xiphophorus maculatus), green swordtail (Xiphophorus hellerei) and sablefish ... |
author2 |
Sherwood, Nancy |
format |
Thesis |
author |
Lescheid, David William |
author_facet |
Lescheid, David William |
author_sort |
Lescheid, David William |
title |
Evolution of three neuropeptides isolated from the brain of sturgeon |
title_short |
Evolution of three neuropeptides isolated from the brain of sturgeon |
title_full |
Evolution of three neuropeptides isolated from the brain of sturgeon |
title_fullStr |
Evolution of three neuropeptides isolated from the brain of sturgeon |
title_full_unstemmed |
Evolution of three neuropeptides isolated from the brain of sturgeon |
title_sort |
evolution of three neuropeptides isolated from the brain of sturgeon |
publishDate |
1997 |
url |
https://dspace.library.uvic.ca//handle/1828/9767 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_relation |
https://dspace.library.uvic.ca//handle/1828/9767 |
op_rights |
Available to the World Wide Web |
_version_ |
1766196611567845376 |