Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids
Co-evolution between parasites and their hosts result in extremely well-orchestrated and intimate relationships that are characterized by remarkable adaptations in the attack response of the parasite and the defense response of the host. To fully understand host-parasite interactions, these adaptati...
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ftuvicpubl:oai:dspace.library.uvic.ca:1828/5978 2023-05-15T17:52:53+02:00 Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids Braden, Laura Marie Jones, Simon Koop, Benjamin F. 2015 application/pdf http://hdl.handle.net/1828/5978 English en eng http://hdl.handle.net/1828/5978 Braden LM, Barker DE, Koop BF, Jones SRM (2012) Comparative defense-associated responses in salmon skin elicited by the ectoparasite Lepeophtheirus salmonis. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 7, 100–9. Braden LM, Koop BF, Jones SRM (2015) Signatures of resistance to Lepeophtheirus salmonis include a Th2-type response at the louse-salmon interface. Developmental & Comparative Immunology, 48, 178–191. Available to the World Wide Web http://creativecommons.org/licenses/by-nc-sa/2.5/ca/ Attribution-NonCommercial-ShareAlike 2.5 Canada CC-BY-NC-SA Immunohistochemistry Transcriptomics Lepeophtheirus salmonis Oncorhynchus gorbuscha Salmo salar Oncorhynchus keta Oncorhynchus nerka Oncorhynchus kisutch Salmon Inflammatory response Host-parasite interactions Resistance Thesis 2015 ftuvicpubl 2022-05-19T06:12:29Z Co-evolution between parasites and their hosts result in extremely well-orchestrated and intimate relationships that are characterized by remarkable adaptations in the attack response of the parasite and the defense response of the host. To fully understand host-parasite interactions, these adaptations must be considered in the context of the ecological constraints in which they evolved. As a serious pest to salmon mariculture, Lepeophtheirus salmonis has been extensively studied; however, there are still several areas that require further research. Of utmost importance, and the topic of this thesis, is molecular basis for resistance to sea lice. The following chapters investigate this phenomena under the umbrella of ecological immunology using combined modern technologies of transcriptomics, proteomics and functional immunology with a focus on the primary interaction site. In the first chapter, I describe the key players involved in this host-parasite relationship with a focus on the primary interaction site, the louse-salmon interface, where there are responses by the louse (attack) and the salmon host (defense). Previous research indicated that an early aggressive inflammatory response at the louse-skin interface contributes to resistance in coho salmon; however, there are no data characterizing a site-specific response in resistant (pink and coho) and susceptible (Atlantic, chum) species. Accordingly in Chapter 2, I define site-specific cutaneous responses in Atlantic, pink and chum salmon to establish genetic biomarkers of resistance. Chapter 3 focuses on identification of cellular effectors using histochemical localization of biomarkers to characterize cellular populations activated at the louse-attachment site, while broadening the gene targets. Our notion of pink salmon as a resistant species is challenged by the common observation of migrating pink salmon supporting large populations of L. salmonis in the field. Thus the purpose of chapter 4 was to investigate potential mechanisms to explain variations ... Thesis Oncorhynchus gorbuscha Pink salmon Salmo salar University of Victoria (Canada): UVicDSpace Keta ENVELOPE(-19.455,-19.455,65.656,65.656) The Louse ENVELOPE(-56.415,-56.415,51.700,51.700) |
institution |
Open Polar |
collection |
University of Victoria (Canada): UVicDSpace |
op_collection_id |
ftuvicpubl |
language |
English |
topic |
Immunohistochemistry Transcriptomics Lepeophtheirus salmonis Oncorhynchus gorbuscha Salmo salar Oncorhynchus keta Oncorhynchus nerka Oncorhynchus kisutch Salmon Inflammatory response Host-parasite interactions Resistance |
spellingShingle |
Immunohistochemistry Transcriptomics Lepeophtheirus salmonis Oncorhynchus gorbuscha Salmo salar Oncorhynchus keta Oncorhynchus nerka Oncorhynchus kisutch Salmon Inflammatory response Host-parasite interactions Resistance Braden, Laura Marie Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids |
topic_facet |
Immunohistochemistry Transcriptomics Lepeophtheirus salmonis Oncorhynchus gorbuscha Salmo salar Oncorhynchus keta Oncorhynchus nerka Oncorhynchus kisutch Salmon Inflammatory response Host-parasite interactions Resistance |
description |
Co-evolution between parasites and their hosts result in extremely well-orchestrated and intimate relationships that are characterized by remarkable adaptations in the attack response of the parasite and the defense response of the host. To fully understand host-parasite interactions, these adaptations must be considered in the context of the ecological constraints in which they evolved. As a serious pest to salmon mariculture, Lepeophtheirus salmonis has been extensively studied; however, there are still several areas that require further research. Of utmost importance, and the topic of this thesis, is molecular basis for resistance to sea lice. The following chapters investigate this phenomena under the umbrella of ecological immunology using combined modern technologies of transcriptomics, proteomics and functional immunology with a focus on the primary interaction site. In the first chapter, I describe the key players involved in this host-parasite relationship with a focus on the primary interaction site, the louse-salmon interface, where there are responses by the louse (attack) and the salmon host (defense). Previous research indicated that an early aggressive inflammatory response at the louse-skin interface contributes to resistance in coho salmon; however, there are no data characterizing a site-specific response in resistant (pink and coho) and susceptible (Atlantic, chum) species. Accordingly in Chapter 2, I define site-specific cutaneous responses in Atlantic, pink and chum salmon to establish genetic biomarkers of resistance. Chapter 3 focuses on identification of cellular effectors using histochemical localization of biomarkers to characterize cellular populations activated at the louse-attachment site, while broadening the gene targets. Our notion of pink salmon as a resistant species is challenged by the common observation of migrating pink salmon supporting large populations of L. salmonis in the field. Thus the purpose of chapter 4 was to investigate potential mechanisms to explain variations ... |
author2 |
Jones, Simon Koop, Benjamin F. |
format |
Thesis |
author |
Braden, Laura Marie |
author_facet |
Braden, Laura Marie |
author_sort |
Braden, Laura Marie |
title |
Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids |
title_short |
Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids |
title_full |
Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids |
title_fullStr |
Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids |
title_full_unstemmed |
Investigating the molecular basis for resistance to the sea louse, Lepeophtheirus salmonis, among salmonids |
title_sort |
investigating the molecular basis for resistance to the sea louse, lepeophtheirus salmonis, among salmonids |
publishDate |
2015 |
url |
http://hdl.handle.net/1828/5978 |
long_lat |
ENVELOPE(-19.455,-19.455,65.656,65.656) ENVELOPE(-56.415,-56.415,51.700,51.700) |
geographic |
Keta The Louse |
geographic_facet |
Keta The Louse |
genre |
Oncorhynchus gorbuscha Pink salmon Salmo salar |
genre_facet |
Oncorhynchus gorbuscha Pink salmon Salmo salar |
op_relation |
http://hdl.handle.net/1828/5978 Braden LM, Barker DE, Koop BF, Jones SRM (2012) Comparative defense-associated responses in salmon skin elicited by the ectoparasite Lepeophtheirus salmonis. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 7, 100–9. Braden LM, Koop BF, Jones SRM (2015) Signatures of resistance to Lepeophtheirus salmonis include a Th2-type response at the louse-salmon interface. Developmental & Comparative Immunology, 48, 178–191. |
op_rights |
Available to the World Wide Web http://creativecommons.org/licenses/by-nc-sa/2.5/ca/ Attribution-NonCommercial-ShareAlike 2.5 Canada |
op_rightsnorm |
CC-BY-NC-SA |
_version_ |
1766160633830572032 |