Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses
We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SE...
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Palynology
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Online Access: | http://hdl.handle.net/1828/12610 https://doi.org/10.1080/01916122.2018.1465736 |
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ftuvicpubl:oai:dspace.library.uvic.ca:1828/12610 2023-05-15T15:39:07+02:00 Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses Nieuwenhove, Nicolas Van Potvin, Éric Heikkilä, Maija Pospelova, Vera Mertens, Kenneth Neil Masure, Edwige Kucharska, Małgorzata Yang, Eun Jin Chomérat, Nicolas Zajaczkowski, Marek 2018 application/pdf http://hdl.handle.net/1828/12610 https://doi.org/10.1080/01916122.2018.1465736 en eng Palynology Nieuwenhove, N. V., Potvin, E., Heikkilä, M., Pospelova, V., Mertens, K. N., Masure, E. …Zajaczkowski, M. (2018). Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses. Palynology, 42(1), 111-134. https://doi.org/10.1080/01916122.2018.1465736 https://doi.org/10.1080/01916122.2018.1465736 http://hdl.handle.net/1828/12610 single-cell PCR morphologic variability taxonomy Article 2018 ftuvicpubl https://doi.org/10.1080/01916122.2018.1465736 2022-05-19T06:11:21Z We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SEM) observations of cysts from Barents Sea surface sediments, and genetic analyses of cysts from the Beaufort Sea. The measurements suggest that populations and morphospecies cannot be distinguished based on morphometric criteria. Furthermore, sequential sediment trap samples from Hudson Bay reveal that morphological variation can occur at the same location over a few weeks, arguing against a uniform morphological response to environmental parameters. The SEM observations reveal a consistent gonyaulacacean tabulation (Po, 4′, 6′′, 6c, 5 s, *6′′′, 1p, 1′′′′). The small and large subunit ribosomal RNA genes and the internal transcribed spacer sequences obtained from differing cysts from the Beaufort Sea with morphologies attributable to the complex, including forms that correspond to Rottnestia amphicavata, were all identical and conform to those of Gonyaulax elongata from the Orkney Islands. The molecular analyses thus support the conclusion that the morphological variability is not reflected genetically and occurs within one species. Based on arguments against the generic attribution of amphicavata to Rottnestia, the continuum between the extreme ends of the morphological range, and the molecular data, we suggest Rottnestia amphicavata to be conspecific with Spiniferites frigidus, and both to be junior synonyms of Spiniferites elongatus. The morphometric data further indicate that Spiniferites ellipsoideus, an elongate cyst from the Miocene, can also be considered a junior synonym of Spiniferites elongatus. It is recommended to use two informal types in census work (i.e. Spiniferites elongatus – Beaufort morphotype for morphologies formerly assignable to Spiniferites frigidus/Rottnestia amphicavata, and Spiniferites ... Article in Journal/Newspaper Barents Sea Beaufort Sea Hudson Bay University of Victoria (Canada): UVicDSpace Barents Sea Hudson Hudson Bay Palynology 42 sup1 111 134 |
institution |
Open Polar |
collection |
University of Victoria (Canada): UVicDSpace |
op_collection_id |
ftuvicpubl |
language |
English |
topic |
single-cell PCR morphologic variability taxonomy |
spellingShingle |
single-cell PCR morphologic variability taxonomy Nieuwenhove, Nicolas Van Potvin, Éric Heikkilä, Maija Pospelova, Vera Mertens, Kenneth Neil Masure, Edwige Kucharska, Małgorzata Yang, Eun Jin Chomérat, Nicolas Zajaczkowski, Marek Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses |
topic_facet |
single-cell PCR morphologic variability taxonomy |
description |
We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SEM) observations of cysts from Barents Sea surface sediments, and genetic analyses of cysts from the Beaufort Sea. The measurements suggest that populations and morphospecies cannot be distinguished based on morphometric criteria. Furthermore, sequential sediment trap samples from Hudson Bay reveal that morphological variation can occur at the same location over a few weeks, arguing against a uniform morphological response to environmental parameters. The SEM observations reveal a consistent gonyaulacacean tabulation (Po, 4′, 6′′, 6c, 5 s, *6′′′, 1p, 1′′′′). The small and large subunit ribosomal RNA genes and the internal transcribed spacer sequences obtained from differing cysts from the Beaufort Sea with morphologies attributable to the complex, including forms that correspond to Rottnestia amphicavata, were all identical and conform to those of Gonyaulax elongata from the Orkney Islands. The molecular analyses thus support the conclusion that the morphological variability is not reflected genetically and occurs within one species. Based on arguments against the generic attribution of amphicavata to Rottnestia, the continuum between the extreme ends of the morphological range, and the molecular data, we suggest Rottnestia amphicavata to be conspecific with Spiniferites frigidus, and both to be junior synonyms of Spiniferites elongatus. The morphometric data further indicate that Spiniferites ellipsoideus, an elongate cyst from the Miocene, can also be considered a junior synonym of Spiniferites elongatus. It is recommended to use two informal types in census work (i.e. Spiniferites elongatus – Beaufort morphotype for morphologies formerly assignable to Spiniferites frigidus/Rottnestia amphicavata, and Spiniferites ... |
format |
Article in Journal/Newspaper |
author |
Nieuwenhove, Nicolas Van Potvin, Éric Heikkilä, Maija Pospelova, Vera Mertens, Kenneth Neil Masure, Edwige Kucharska, Małgorzata Yang, Eun Jin Chomérat, Nicolas Zajaczkowski, Marek |
author_facet |
Nieuwenhove, Nicolas Van Potvin, Éric Heikkilä, Maija Pospelova, Vera Mertens, Kenneth Neil Masure, Edwige Kucharska, Małgorzata Yang, Eun Jin Chomérat, Nicolas Zajaczkowski, Marek |
author_sort |
Nieuwenhove, Nicolas Van |
title |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses |
title_short |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses |
title_full |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses |
title_fullStr |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses |
title_full_unstemmed |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses |
title_sort |
taxonomic revision of spiniferites elongatus (the resting stage of gonyaulax elongata) based on morphological and molecular analyses |
publisher |
Palynology |
publishDate |
2018 |
url |
http://hdl.handle.net/1828/12610 https://doi.org/10.1080/01916122.2018.1465736 |
geographic |
Barents Sea Hudson Hudson Bay |
geographic_facet |
Barents Sea Hudson Hudson Bay |
genre |
Barents Sea Beaufort Sea Hudson Bay |
genre_facet |
Barents Sea Beaufort Sea Hudson Bay |
op_relation |
Nieuwenhove, N. V., Potvin, E., Heikkilä, M., Pospelova, V., Mertens, K. N., Masure, E. …Zajaczkowski, M. (2018). Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses. Palynology, 42(1), 111-134. https://doi.org/10.1080/01916122.2018.1465736 https://doi.org/10.1080/01916122.2018.1465736 http://hdl.handle.net/1828/12610 |
op_doi |
https://doi.org/10.1080/01916122.2018.1465736 |
container_title |
Palynology |
container_volume |
42 |
container_issue |
sup1 |
container_start_page |
111 |
op_container_end_page |
134 |
_version_ |
1766370563200122880 |